Reviewed, UniProtKB/Swiss-Prot O67791 (SUHB_AQUAE)

Names and origin

Protein names

Recommended name:
    Inositol-1-monophosphatase
      Short name=IMPase
      Short name=Inositol-1-phosphatase
      Short name=I-1-Pase
    EC=3.1.3.25

Gene names

Name:	suhB
Synonyms:	imp2
Ordered Locus Names:	aq_1983

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Myo-inositol phosphate + H2O = myo-inositol + phosphate.
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Molecular function	inositol-1(or 4)-monophosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 264

264

Inositol-1-monophosphatase

PRO_0000142553

Regions

Region

88 – 91

4

Substrate binding By similarity

Sites

Metal binding

70

1

Magnesium 1 By similarity

Metal binding

86

1

Magnesium 1 By similarity

Metal binding

86

1

Magnesium 2 By similarity

Metal binding

88

1

Magnesium 1; via carbonyl oxygen By similarity

Metal binding

89

1

Magnesium 2 By similarity

Metal binding

214

1

Magnesium 2 By similarity

Binding site

70

1

Substrate By similarity

Binding site

157

1

Substrate By similarity

Binding site

185

1

Substrate By similarity

Binding site

214

1

Substrate By similarity

Secondary structure

1

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264

Helix Strand Turn

Details...

Helix

4 – 25

22

Helix

45 – 61

17

Beta strand

65 – 68

4

Beta strand

81 – 89

9

Helix

91 – 96

6

Beta strand

102 – 109

8

Beta strand

112 – 120

9

Turn

121 – 124

4

Beta strand

125 – 130

6

Turn

131 – 133

3

Beta strand

134 – 137

4

Beta strand

140 – 142

3

Helix

150 – 152

3

Beta strand

154 – 158

5

Helix

167 – 180

14

Beta strand

181 – 186

6

Helix

190 – 198

9

Beta strand

201 – 210

10

Helix

212 – 224

13

Beta strand

228 – 233

6

Beta strand

237 – 245

9

Helix

246 – 259

14

Sequences

	Sequence		Length	Mass (Da)
	O67791-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 10F32D1D3B81B76A	FASTA	264	29,336
	10 20 30 40 50 60 MENLKKYLEV AKIAALAGGQ VLKENFGKVK KENIEEKGEK DFVSYVDKTS EERIKEVILK 70 80 90 100 110 120 FFPDHEVVGE EMGAEGSGSE YRWFIDPLDG TKNYINGFPI FAVSVGLVKG EEPIVGAVYL 130 140 150 160 170 180 PYFDKLYWGA KGLGAYVNGK RIKVKDNESL KHAGVVYGFP SRSRRDISIY LNIFKDVFYE 190 200 210 220 230 240 VGSMRRPGAA AVDLCMVAEG IFDGMMEFEM KPWDITAGLV ILKEAGGVYT LVGEPFGVSD 250 260 IIAGNKALHD FILQVAKKYM EVAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
[2]	"Crystal structure of myo-inositol-1(or 4)-monophosphatase (aq_1983) from Aquifex aeolicus VF5." RIKEN structural genomics initiative (RSGI) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000657 Genomic DNA. Translation: AAC07753.1.

PIR

C70470.

RefSeq

NP_214360.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PCR	X-ray	2.60	A/B/C/D	1-264	[»]

ModBase

Search...

Genome annotation databases

GeneID

1193688.

GenomeReviews

Gene locus aq_1983 in contig AE000657_GR.

KEGG

aae:aq_1983.

NMPDR

fig|224324.1.peg.1375.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG730251.

OMA

YLTHKTR.

PhylomeDB

O67791.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_1983-MONOMER.

BRENDA

3.1.3.25. 189781.

Family and domain databases

InterPro

IPR020583. Inositol_monoP_metal-BS.
IPR000760. Inositol_monophosphatase.
[Graphical view]

PANTHER

PTHR20854. Inositol_P. 1 hit.

Pfam

PF00459. Inositol_P. 1 hit.
[Graphical view]

PRINTS

PR00377. IMPHPHTASES.

PROSITE

PS00629. IMP_1. 1 hit.
PS00630. IMP_2. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SUHB_AQUAE

Accession

Primary (citable) accession number: O67791

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	August 1, 1998
Last modified:	January 19, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)