O67783 (PANB_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase
EC=2.1.2.11

Alternative name(s):
Ketopantoate hydroxymethyltransferase
Short name=KPHMT

Gene names

Name:	panB
Ordered Locus Names:	aq_1973

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP MF_00156
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00156
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity. HAMAP MF_00156
Subcellular location	Cytoplasm Potential HAMAP MF_00156.
Sequence similarities	Belongs to the PanB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156		PRO_0000184807
Regions
	Region	44 – 45	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	182	1	Proton acceptor By similarity
	Metal binding	44	1	Magnesium By similarity
	Metal binding	83	1	Magnesium By similarity
	Metal binding	115	1	Magnesium By similarity
	Binding site	83	1	Alpha-ketoisovalerate By similarity
	Binding site	113	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67783 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C5D2FAAFB443C83D	FASTA	265	29,513
	10 20 30 40 50 60 MAINLQTLFK KKREGKKITM VSTYDYWSAK LCDEVGIDCI LVGDSLGTVV KGEGDTLSVT 70 80 90 100 110 120 LEEIIYHTKC VMRGVKNAFV IADMPFMSYQ VSMEKAVENC GRVIKETKAK AVKLEGGEEI 130 140 150 160 170 180 AELVYKLTRI GIPVVGHVGF TPQHINVFGK PKVVGKKKEE EEKLRRDFRA LEEAGAFMIV 190 200 210 220 230 240 LESVPTHLAK ELWKGSNSIV IGIGAGKYVD GQVLVFHDIV GLFEDFKPKF VRRYLEGAKL 250 260 VKEALKNFKI DVEGGNFPSE EESYG

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07749.1.
PIR	C70469.
RefSeq	NP_214352.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67783.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193680.
GenomeReviews	Gene locus aq_1973 in contig AE000657_GR.
KEGG	aae:aq_1973.
NMPDR	fig|224324.1.peg.1367.
PATRIC	20960856. VBIAquAeo85532_1523.
Phylogenomic databases
HOGENOM	HBG299908.
OMA	MAHVGLM.
PhylomeDB	O67783.
ProtClustDB	PRK00311.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1973-MONOMER.
Family and domain databases
HAMAP	MF_00156. PanB. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K00606.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR00222. PanB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB_AQUAE

Accession

Primary (citable) accession number: O67783

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program