Reviewed, UniProtKB/Swiss-Prot O67775 (PUR9_AQUAE)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	aq_1963

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_0000192068

Sequences

	Sequence		Length	Mass (Da)
	O67775-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 46998C8B1B1FE694	FASTA	506	56,677
	10 20 30 40 50 60 MRAIISVYRK EGIDKLAKAL QELGYEIVST GGTAKYLREK GISVKEVSEI TGFPEILEGR 70 80 90 100 110 120 VKTLHPVVHG GILFRDWVEK DKEEIEKHGI KPIDVVVVNL YPFEEKLKEG LTDKDLMEFI 130 140 150 160 170 180 DIGGPTLIRA AAKNFFRVVI LVDPEDYDWV IEKLKKGNLT LQDRAYLAWK AFSHTAYYDG 190 200 210 220 230 240 VISQAFKKLY SIDTFGKEEA LPLKRMQKLR YGENPHQRGF LYENPLEDIG ITKAQVLQGK 250 260 270 280 290 300 EMSFNNYLDA DSAVRLVAEF PNQTVCAIIK HNNPCGVALG SSVKEAFLRA KEADPVSAFG 310 320 330 340 350 360 GIVAFNDKVD GETAKELTSM FLEVVIAPDY DEEALRELSR KKNLRVIRFF GFQHAFDVKK 370 380 390 400 410 420 VSGGYLLQDE DTVLYEKLQV VTKREPTAEE MEDLLFAWKV VKHTKSNAVV IAKNGQTLGI 430 440 450 460 470 480 GSGNVSRVDS LKCAINKAKE FGFDLKGAVV ASEAFFPFRD SIDIMAKEGI TAVIQPGGSI 490 500 RDQEVIDACN EHGIAMIFTG LRHFKH

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07734.1.
PIR	C70468.
RefSeq	NP_214344.1.
3D structure databases
SMR	O67775. Positions 2-506.
ModBase	Search...
Genome annotation databases
GeneID	1193672.
GenomeReviews	Gene locus aq_1963 in contig AE000657_GR.
KEGG	aae:aq_1963.
NMPDR	fig|224324.1.peg.1359.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG498048.
OMA	VVKHVKS.
PhylomeDB	O67775.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1963-MONOMER.
BRENDA	2.1.2.3. 189781. 3.5.4.10. 189781.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_AQUAE

Accession

Primary (citable) accession number: O67775

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)