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O67766 (RUSA_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RusA

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase
Gene names
Name:rusA
Ordered Locus Names:aq_1953
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length126 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Ref.2

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the RusA family.

Sequence caution

The sequence AAC07739.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 126126Crossover junction endodeoxyribonuclease RusA
PRO_0000192008

Sites

Metal binding761Magnesium By similarity
Metal binding781Magnesium By similarity
Metal binding971Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
O67766 [UniParc].

Last modified August 29, 2001. Version 2.
Checksum: 05BC64FAA7B9F9B1

FASTA12614,778
        10         20         30         40         50         60 
MISEMEEIEL YLSKLPVPKS NRYIRPKGGK VFKPPRVTNW EARAIWELRE QFKGDAIDYE 

        70         80         90        100        110        120 
ISVDITLILP NRRKRDIDNM LKSLWDVMEK AGVIKNDNLI FEVHTVKKIE KGKEGVIIKI 


RPFQSP 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.
[2]"RusA proteins from the extreme thermophile Aquifex aeolicus and lactococcal phage r1t resolve Holliday junctions."
Sharples G.J., Bolt E.L., Lloyd R.G.
Mol. Microbiol. 44:549-559(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC07739.1. Different initiation.
PIRB70467.
RefSeqNP_214335.1. NC_000918.1.

3D structure databases

ProteinModelPortalO67766.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_1953.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC07739; AAC07739; aq_1953.
GeneID1193663.
KEGGaae:aq_1953.
PATRIC20960818. VBIAquAeo85532_1505.

Phylogenomic databases

eggNOGCOG4570.
HOGENOMHOG000119986.
KOK01160.
OMAKRDIDNM.
OrthoDBEOG61CM6B.
ProtClustDBCLSK2299879.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-1395-MONOMER.

Family and domain databases

Gene3D3.30.1330.70. 1 hit.
InterProIPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamPF05866. RusA. 1 hit.
[Graphical view]
SUPFAMSSF103084. SSF103084. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUSA_AQUAE
AccessionPrimary (citable) accession number: O67766
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 29, 2001
Last modified: April 16, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families