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O67766

- RUSA_AQUAE

UniProt

O67766 - RUSA_AQUAE

Protein

Crossover junction endodeoxyribonuclease RusA

Gene

rusA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides.1 Publication

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi76 – 761MagnesiumBy similarity
    Metal bindingi78 – 781MagnesiumBy similarity
    Metal bindingi97 – 971MagnesiumBy similarity

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1395-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RusA (EC:3.1.22.4)
    Alternative name(s):
    Holliday junction nuclease RusA
    Holliday junction resolvase
    Gene namesi
    Name:rusA
    Ordered Locus Names:aq_1953
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 126126Crossover junction endodeoxyribonuclease RusAPRO_0000192008Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi224324.aq_1953.

    Structurei

    3D structure databases

    ProteinModelPortaliO67766.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RusA family.Curated

    Phylogenomic databases

    eggNOGiCOG4570.
    HOGENOMiHOG000119986.
    KOiK01160.
    OMAiKRDIDNM.
    OrthoDBiEOG61CM6B.

    Family and domain databases

    Gene3Di3.30.1330.70. 1 hit.
    InterProiIPR008822. Endonuclease_RusA-like.
    [Graphical view]
    PfamiPF05866. RusA. 1 hit.
    [Graphical view]
    SUPFAMiSSF103084. SSF103084. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O67766-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISEMEEIEL YLSKLPVPKS NRYIRPKGGK VFKPPRVTNW EARAIWELRE    50
    QFKGDAIDYE ISVDITLILP NRRKRDIDNM LKSLWDVMEK AGVIKNDNLI 100
    FEVHTVKKIE KGKEGVIIKI RPFQSP 126
    Length:126
    Mass (Da):14,778
    Last modified:August 29, 2001 - v2
    Checksum:i05BC64FAA7B9F9B1
    GO

    Sequence cautioni

    The sequence AAC07739.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07739.1. Different initiation.
    PIRiB70467.
    RefSeqiNP_214335.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07739; AAC07739; aq_1953.
    GeneIDi1193663.
    KEGGiaae:aq_1953.
    PATRICi20960818. VBIAquAeo85532_1505.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07739.1 . Different initiation.
    PIRi B70467.
    RefSeqi NP_214335.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O67766.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_1953.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07739 ; AAC07739 ; aq_1953 .
    GeneIDi 1193663.
    KEGGi aae:aq_1953.
    PATRICi 20960818. VBIAquAeo85532_1505.

    Phylogenomic databases

    eggNOGi COG4570.
    HOGENOMi HOG000119986.
    KOi K01160.
    OMAi KRDIDNM.
    OrthoDBi EOG61CM6B.

    Enzyme and pathway databases

    BioCyci AAEO224324:GJBH-1395-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1330.70. 1 hit.
    InterProi IPR008822. Endonuclease_RusA-like.
    [Graphical view ]
    Pfami PF05866. RusA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103084. SSF103084. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.
    2. "RusA proteins from the extreme thermophile Aquifex aeolicus and lactococcal phage r1t resolve Holliday junctions."
      Sharples G.J., Bolt E.L., Lloyd R.G.
      Mol. Microbiol. 44:549-559(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiRUSA_AQUAE
    AccessioniPrimary (citable) accession number: O67766
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 29, 2001
    Last sequence update: August 29, 2001
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3