O67762 (RPOB_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	aq_1939

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	1468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1468	1468	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047855

Sequences

	Sequence		Length	Mass (Da)
	O67762 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 9182372EA417930E	FASTA	1,468	167,388
	10 20 30 40 50 60 MAKIALPRKF FGRRFEVLEP PYLLSIPKNS FENFVQLKVN PYKRKNVGLE HIFRTSFPFK 70 80 90 100 110 120 DPDENFILEY LGYEIGDWEC NRCGYKPKDD LLGGWDVDCP QCGAKLVYKE KFTPEECKLK 130 140 150 160 170 180 GLTYSAPLRV MLQLKAKTKN GYREFPPKKV YFGEIPLMTE TGSFIINGTE RIIINQLIRS 190 200 210 220 230 240 SGVFFDEKEE KQKDATITRI LYRGSIIPDK GSRVEFELSG ATDLISARID RKKLSATAVL 250 260 270 280 290 300 RAFGLETAYD ILKYFYEDVR KFIVKDKYLY DAQTGEEFTP EDLEHHYIFA IIKFRGKLVG 310 320 330 340 350 360 FASSKERDFI EERYIEEWED LVRLLDDDRI EVLSVTAVPR DVVIKSPYGK SLIDTLANDT 370 380 390 400 410 420 SPKDVDKRSP VKVPAEYTLR DYGLMEMYKR LRHIEAITME IDSVIERARI FFNVFFRDLK 430 440 450 460 470 480 RYDLSRVGRV KINAKVHRIP KVLKPADVDL LDQLPPLALA EDYGEYKAGT RVTKDLLKEL 490 500 510 520 530 540 FKQYKEIKVK DYTEDEARFI LPIDLVNILK YLIDLRHGRV KKDDIAHLGN RRVRSVGELL 550 560 570 580 590 600 ENQARLGIAK MEKVFRDRSA VINPEQPDLK PQDFINPRYV TTAITDFLKT GQLSQYLDNT 610 620 630 640 650 660 NPLSELTHKR RLSALGPGGL TRESAKFEIR DVHPSHYGRI CPIETPEGQN IGLVTSPTVY 670 680 690 700 710 720 ARVNEYGFLI TPYRKVENGK VTDKIEWLAA YEEENYVIAQ STPTDEEGRL KAEFILARHK 730 740 750 760 770 780 NDIRLVKPEQ VEYIDVSPRQ VISPSSSLIP FLEHDDANRA LMGSNMQRQA VPLIFTQAPL 790 800 810 820 830 840 IGTGMEKKIA RDSHAVVVAK RGGVVEEVDS SKIIIRVNPE EINFDDPLDI GIDIYELRKF 850 860 870 880 890 900 QRTNQKTCVN QRPIVRKGEK VEKGQIIADG HSTDRGELAL GKDVLVAFMP WRGYNFEDAI 910 920 930 940 950 960 VISERLVKED VYTSIHIEEL EVEARETKVG EEEITRQIPG VPERALAHLD EHGIVRVGTY 970 980 990 1000 1010 1020 VKPGDILVGK VTPKGETRLT PEEKLLQAIF GEKTRDVKDA SLRCPPGVEG IVIDVQVFTR 1030 1040 1050 1060 1070 1080 KGTGKKDMLA EKVEREELEA LEQELEKKKN LIITGRDKVL KGLVLGRKVE KDAKVGRKTV 1090 1100 1110 1120 1130 1140 KKGTVIDEKV FEEFVNYILG RPENFFEDED LIRKIREIAE RTRTQIEMLN KVYEEKKETL 1150 1160 1170 1180 1190 1200 LKRRDLPPGV ITLVKVFIAN KRKIKVGDKM AGRHGNKGVI SVVLPVEDMP FLPDGTPVDI 1210 1220 1230 1240 1250 1260 VLNPLGVPSR MNVGQILETH LGWAAKELGK KIGEMIEKGR DRKAITEYLK EIFAIGDKDG 1270 1280 1290 1300 1310 1320 ENAKFIEEFL NSLSEEEFWS VVRDYAERGI PMATPAFEGA EEDAIKELLK KAGLPENGKT 1330 1340 1350 1360 1370 1380 TLYDGRTGEP FDFEVTVGYM HMLKLIHMVD DKIHARATGP YSLVTQQPLG GRAQFGGQRL 1390 1400 1410 1420 1430 1440 GEMEVWALEA HGAAYTLQEM LTVKSDDVEG RTKVYEAIVK GKYTYTPGIP ESFKVLVREL 1450 1460 KALGLNVKCL NGEEKPCDEV EVKEEEEK

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07723.1.
PIR	F70466.
RefSeq	NP_214331.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67762.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193659.
GenomeReviews	Gene locus aq_1939 in contig AE000657_GR.
KEGG	aae:aq_1939.
NMPDR	fig|224324.1.peg.1346.
PATRIC	20960810. VBIAquAeo85532_1501.
Phylogenomic databases
HOGENOM	HBG285547.
OMA	IGRNKTY.
PhylomeDB	O67762.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1939-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_AQUAE

Accession

Primary (citable) accession number: O67762

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program