O67742 (PYRE_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	aq_1907

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110663
Regions
	Region	126 – 134	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	100	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	101	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	104	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	106	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	130	1	Orotate By similarity
	Binding site	158	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67742 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 696A9B0F9B68316E	FASTA	183	20,310
	10 20 30 40 50 60 MLMLEREKLA RLIKKRSLKV ADEPVFKLSS GKLSRYYVDL KQITFDPEGD YLIGKAMYEL 70 80 90 100 110 120 VKEFNPDACG GLTLGADPIA YAIAFVSLMD SNPIKPFVVR KEPKGHGMKR QIEGLLNPGE 130 140 150 160 170 180 RVAVLEDVVT TGSSALKAVK ACREYGLEVI GVFAVVDREE GGRENIEKEG IPLYSLFKLS ELL

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07702.1.
PIR	B70464.
RefSeq	NP_214310.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67742.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193547.
GenomeReviews	Gene locus aq_1907 in contig AE000657_GR.
KEGG	aae:aq_1907.
NMPDR	fig|224324.1.peg.1325.
PATRIC	20960750. VBIAquAeo85532_1476.
Phylogenomic databases
HOGENOM	HBG404341.
OMA	GKEADYY.
PhylomeDB	O67742.
ProtClustDB	CLSK2299868.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1907-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_AQUAE

Accession

Primary (citable) accession number: O67742

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program