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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi32 – 37ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.4.19. 396.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:aq_1887
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001816391 – 317tRNA(Ile)-lysidine synthaseAdd BLAST317

Interactioni

Protein-protein interaction databases

DIPiDIP-29530N.
STRINGi224324.aq_1887.

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 18Combined sources16
Beta strandi26 – 30Combined sources5
Helixi35 – 46Combined sources12
Turni48 – 52Combined sources5
Beta strandi54 – 62Combined sources9
Helixi68 – 83Combined sources16
Beta strandi87 – 91Combined sources5
Helixi94 – 100Combined sources7
Helixi105 – 123Combined sources19
Beta strandi127 – 130Combined sources4
Helixi135 – 148Combined sources14
Helixi152 – 156Combined sources5
Beta strandi160 – 162Combined sources3
Turni168 – 171Combined sources4
Helixi174 – 183Combined sources10
Helixi192 – 195Combined sources4
Helixi199 – 206Combined sources8
Helixi208 – 215Combined sources8
Helixi219 – 248Combined sources30
Beta strandi253 – 255Combined sources3
Helixi256 – 259Combined sources4
Helixi264 – 275Combined sources12
Helixi280 – 287Combined sources8
Helixi288 – 290Combined sources3
Beta strandi295 – 305Combined sources11
Beta strandi308 – 310Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WY5X-ray2.42A/B1-317[»]
2E21X-ray2.70A/B/C/D1-317[»]
2E89X-ray2.50A/B/C/D1-317[»]
ProteinModelPortaliO67728.
SMRiO67728.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67728.

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000071854.
InParanoidiO67728.
KOiK04075.
OMAiCREWEVE.
OrthoDBiPOG091H02FP.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

O67728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPESRVIRK VLALQNDEKI FSGERRVLIA FSGGVDSVVL TDVLLKLKNY
60 70 80 90 100
FSLKEVALAH FNHMLRESAE RDEEFCKEFA KERNMKIFVG KEDVRAFAKE
110 120 130 140 150
NRMSLEEAGR FLRYKFLKEI LESEGFDCIA TAHHLNDLLE TSLLFFTRGT
160 170 180 190 200
GLDGLIGFLP KEEVIRRPLY YVKRSEIEEY AKFKGLRWVE DETNYEVSIP
210 220 230 240 250
RNRIRHRVIP ELKRINENLE DTFLKMVKVL RAEREFLEEE AQKLYKEVKK
260 270 280 290 300
GNCLDVKKLK EKPLALQRRV IRKFIGEKDY EKVELVRSLL EKGGEVNLGK
310
GKVLKRKERW LCFSPEV
Length:317
Mass (Da):37,320
Last modified:August 1, 1998 - v1
Checksum:i78D8C6229152A296
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07685.1.
PIRiF70462.
RefSeqiNP_214296.1. NC_000918.1.
WP_010881232.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07685; AAC07685; aq_1887.
GeneIDi1193535.
KEGGiaae:aq_1887.
PATRICi20960722. VBIAquAeo85532_1462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07685.1.
PIRiF70462.
RefSeqiNP_214296.1. NC_000918.1.
WP_010881232.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WY5X-ray2.42A/B1-317[»]
2E21X-ray2.70A/B/C/D1-317[»]
2E89X-ray2.50A/B/C/D1-317[»]
ProteinModelPortaliO67728.
SMRiO67728.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29530N.
STRINGi224324.aq_1887.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07685; AAC07685; aq_1887.
GeneIDi1193535.
KEGGiaae:aq_1887.
PATRICi20960722. VBIAquAeo85532_1462.

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000071854.
InParanoidiO67728.
KOiK04075.
OMAiCREWEVE.
OrthoDBiPOG091H02FP.

Enzyme and pathway databases

BRENDAi6.3.4.19. 396.

Miscellaneous databases

EvolutionaryTraceiO67728.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTILS_AQUAE
AccessioniPrimary (citable) accession number: O67728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.