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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 376ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1351-MONOMER.
BRENDAi6.3.4.19. 396.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:aq_1887
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317tRNA(Ile)-lysidine synthasePRO_0000181639Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-29530N.
STRINGi224324.aq_1887.

Structurei

Secondary structure

1
317
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1816Combined sources
Beta strandi26 – 305Combined sources
Helixi35 – 4612Combined sources
Turni48 – 525Combined sources
Beta strandi54 – 629Combined sources
Helixi68 – 8316Combined sources
Beta strandi87 – 915Combined sources
Helixi94 – 1007Combined sources
Helixi105 – 12319Combined sources
Beta strandi127 – 1304Combined sources
Helixi135 – 14814Combined sources
Helixi152 – 1565Combined sources
Beta strandi160 – 1623Combined sources
Turni168 – 1714Combined sources
Helixi174 – 18310Combined sources
Helixi192 – 1954Combined sources
Helixi199 – 2068Combined sources
Helixi208 – 2158Combined sources
Helixi219 – 24830Combined sources
Beta strandi253 – 2553Combined sources
Helixi256 – 2594Combined sources
Helixi264 – 27512Combined sources
Helixi280 – 2878Combined sources
Helixi288 – 2903Combined sources
Beta strandi295 – 30511Combined sources
Beta strandi308 – 3103Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WY5X-ray2.42A/B1-317[»]
2E21X-ray2.70A/B/C/D1-317[»]
2E89X-ray2.50A/B/C/D1-317[»]
ProteinModelPortaliO67728.
SMRiO67728. Positions 1-317.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67728.

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000071854.
InParanoidiO67728.
KOiK04075.
OMAiCREWEVE.
OrthoDBiEOG693GNP.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

O67728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPESRVIRK VLALQNDEKI FSGERRVLIA FSGGVDSVVL TDVLLKLKNY
60 70 80 90 100
FSLKEVALAH FNHMLRESAE RDEEFCKEFA KERNMKIFVG KEDVRAFAKE
110 120 130 140 150
NRMSLEEAGR FLRYKFLKEI LESEGFDCIA TAHHLNDLLE TSLLFFTRGT
160 170 180 190 200
GLDGLIGFLP KEEVIRRPLY YVKRSEIEEY AKFKGLRWVE DETNYEVSIP
210 220 230 240 250
RNRIRHRVIP ELKRINENLE DTFLKMVKVL RAEREFLEEE AQKLYKEVKK
260 270 280 290 300
GNCLDVKKLK EKPLALQRRV IRKFIGEKDY EKVELVRSLL EKGGEVNLGK
310
GKVLKRKERW LCFSPEV
Length:317
Mass (Da):37,320
Last modified:August 1, 1998 - v1
Checksum:i78D8C6229152A296
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07685.1.
PIRiF70462.
RefSeqiNP_214296.1. NC_000918.1.
WP_010881232.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07685; AAC07685; aq_1887.
GeneIDi1193535.
KEGGiaae:aq_1887.
PATRICi20960722. VBIAquAeo85532_1462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07685.1.
PIRiF70462.
RefSeqiNP_214296.1. NC_000918.1.
WP_010881232.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WY5X-ray2.42A/B1-317[»]
2E21X-ray2.70A/B/C/D1-317[»]
2E89X-ray2.50A/B/C/D1-317[»]
ProteinModelPortaliO67728.
SMRiO67728. Positions 1-317.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29530N.
STRINGi224324.aq_1887.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07685; AAC07685; aq_1887.
GeneIDi1193535.
KEGGiaae:aq_1887.
PATRICi20960722. VBIAquAeo85532_1462.

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000071854.
InParanoidiO67728.
KOiK04075.
OMAiCREWEVE.
OrthoDBiEOG693GNP.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1351-MONOMER.
BRENDAi6.3.4.19. 396.

Miscellaneous databases

EvolutionaryTraceiO67728.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiTILS_AQUAE
AccessioniPrimary (citable) accession number: O67728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.