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Protein

Beta sliding clamp

Gene

dnaN

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Beta sliding clamp
Short name:
Beta clamp
Short name:
Sliding clamp
Alternative name(s):
Beta-clamp processivity factor
DNA polymerase III beta sliding clamp subunit
DNA polymerase III subunit beta
Gene namesi
Name:dnaN
Ordered Locus Names:aq_1882
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054221 – 363Beta sliding clampAdd BLAST363

Proteomic databases

PRIDEiO67725.

Interactioni

Subunit structurei

Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.By similarity

Protein-protein interaction databases

STRINGi224324.aq_1882.

Structurei

3D structure databases

ProteinModelPortaliO67725.
SMRiO67725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta sliding clamp family.Curated

Phylogenomic databases

eggNOGiENOG4105CZ8. Bacteria.
COG0592. LUCA.
HOGENOMiHOG000071791.
InParanoidiO67725.
KOiK02338.
OMAiNYEAVIP.
OrthoDBiPOG091H02B3.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiView protein in InterPro
IPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
PANTHERiPTHR30478:SF1. PTHR30478:SF1. 1 hit.
PfamiView protein in Pfam
PF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
PIRSFiPIRSF000804. DNA_pol_III_b. 1 hit.
SMARTiView protein in SMART
SM00480. POL3Bc. 1 hit.
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

O67725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVKVDREEL EEVLKKARES TEKKAALPIL ANFLLSAKEE NLIVRATDLE
60 70 80 90 100
NYLVVSVKGE VEEEGEVCVH SQKLYDIVKN LNSAYVYLHT EGEKLVITGG
110 120 130 140 150
KSTYKLPTAP AEDFPEFPEI VEGGETLSGN LLVNGIEKVE YAIAKEEANI
160 170 180 190 200
ALQGMYLRGY EDRIHFVGSD GHRLALYEPL GEFSKELLIP RKSLKVLKKL
210 220 230 240 250
ITGIEDVNIE KSEDESFAYF STPEWKLAVR LLEGEFPDYM SVIPEEFSAE
260 270 280 290 300
VLFETEEVLK VLKRLKALSE GKVFPVKITL SENLAIFEFA DPEFGEAREE
310 320 330 340 350
IEVEYTGEPF EIGFNGKYLM EALDAYDSER VWFKFTTPDT ATLLEAEDYE
360
KEPYKCIIMP MRV
Length:363
Mass (Da):41,300
Last modified:August 1, 1998 - v1
Checksum:iDD077039BE4E5A5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07688.1.
PIRiC70462.
RefSeqiNP_214293.1. NC_000918.1.
WP_010881229.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07688; AAC07688; aq_1882.
GeneIDi1193532.
KEGGiaae:aq_1882.
PATRICifig|224324.8.peg.1458.

Similar proteinsi

Entry informationi

Entry nameiDPO3B_AQUAE
AccessioniPrimary (citable) accession number: O67725
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: September 27, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families