Reviewed, UniProtKB/Swiss-Prot O67716 (DHAS_AQUAE)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:	asd
Ordered Locus Names:	aq_1866

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Aspartate-semialdehyde dehydrogenase		PRO_0000141359
Sites
	Active site	131	1	Acyl-thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67716-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5B2172ABA2AC190D	FASTA	340	37,799
	10 20 30 40 50 60 MGYRVAIVGA TGEVGRTFLK VLEERNFPVD ELVLYASERS EGKVLTFKGK EYTVKALNKE 70 80 90 100 110 120 NSFKGIDIAL FSAGGSTSKE WAPKFAKDGV VVIDNSSAWR MDPDVPLVVP EVNPEDVKDF 130 140 150 160 170 180 KKKGIIANPN CSTIQMVVAL KPIYDKAGIK RVVVSTYQAV SGAGAKAIED LKNQTKAWCE 190 200 210 220 230 240 GKEMPKAQKF PHQIAFNALP HIDVFFEDGY TKEENKMLYE TRKIMHDENI KVSATCVRIP 250 260 270 280 290 300 VFYGHSESIS METEKEISPE EAREVLKNAP GVIVIDNPQN NEYPMPIMAE GRDEVFVGRI 310 320 330 340 RKDRVFEPGL SMWVVADNIR KGAATNAVQI AELLVKEGLI

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07674.1.
PIR	B70461.
RefSeq	NP_214284.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1193523.
GenomeReviews	Gene locus aq_1866 in contig AE000657_GR.
KEGG	aae:aq_1866.
NMPDR	fig|224324.1.peg.1299.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67716.
OMA	O67716. YLFASKR.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1866-MON.
BRENDA	1.2.1.11. 189781.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR012080. Asp_semialdehyde_DH. IPR005986. Asp_semialdehyde_DH_bac. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR01296. asd_B. 1 hit.
PROSITE	PS01103. ASD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_AQUAE

Accession

Primary (citable) accession number: O67716

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)