Reviewed, UniProtKB/Swiss-Prot O67691 (PURL_AQUAE)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase 2
    EC=6.3.5.3
Alternative name(s):
    Phosphoribosylformylglycinamidine synthase II
      Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	aq_1836

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	745 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, purQ and purL. HAMAP MF_00420
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 745	745	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100431
Regions
	Nucleotide binding	98 – 109	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O67691-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5C0D7B0C89DD4A8F	FASTA	745	83,213
	10 20 30 40 50 60 MERVWEAYGL TEEEYRKILK TLKREPNHVE LGVLGALWSE HCSYKSSKKH LKKFPTKAEW 70 80 90 100 110 120 VVQGPGENAG VVKIDEKVWV AFKVESHNHP SYIEPFHGAA TGVGGIIRDV LSMGARPIAL 130 140 150 160 170 180 ADSLRFGEFN YHETKRLVKG VVSGISFYGN CIGVPTVAGE TVFEPSYKTN PLVNAFCLGV 190 200 210 220 230 240 IPAGRMYRAR ATREGQLLFL IGSSTGRDGI FGAVMASGEF SEDVEEKRPN VQIGDPYFGK 250 260 270 280 290 300 KLVEAIMEIV EKDLIVGMQD LGAAGLAGSA SEIAAKSEKG VELYLENVPL REKDMNPYEI 310 320 330 340 350 360 LLSESQERML LVVEEENVEK VKEIANKWHL EGAVVGKITD DDTFRAYYKG ELVAELPVSL 370 380 390 400 410 420 IVDEAPVYDR PYKEPEYMKE VRNFNQEELP QTDVKEALKK LLSSPNISCK EWVYTQYDYQ 430 440 450 460 470 480 VGTNTLLIPG HDAAVLRLKW VLRPELTTEK GIAISSEGNG RMVYLNPYEG GKFVVAEVCR 490 500 510 520 530 540 NLACVGAKPL AITDCLNFGN PERPEIMWQF VKAVEGMAEA CEELGIPVVS GNVSLYNETV 550 560 570 580 590 600 EKNEIRNVFP TPIVVGVGVL EKAEKYTPSK VEKESELYLV GNLEENLRLD GSEYLKVIHG 610 620 630 640 650 660 LIKGDVPPVD LEKEKILINL LISFNNKELI TCAHDVSVGG LLIALLEMVF RTPYGLEVEV 670 680 690 700 710 720 YTDERPDVFF FSENPTRVII GVESDKAEEV KNAVEKAGLE WMYIGKTTEE KKIKVTFNGD 730 740 TLLEDELEEY EKLWRTSLEK LLGST

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07653.1.
PIR	A70458.
RefSeq	NP_214258.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1193498.
GenomeReviews	Gene locus aq_1836 in contig AE000657_GR.
KEGG	aae:aq_1836.
NMPDR	fig|224324.1.peg.1273.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67691.
OMA	GNCVGVP.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1836-MON.
BRENDA	6.3.5.3. 189781.
Family and domain databases
HAMAP	MF_00420. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_AQUAE

Accession

Primary (citable) accession number: O67691

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)