O67687 (ALR_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	aq_1827

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Alanine racemase HAMAP MF_01201		PRO_0000114495
Sites
	Active site	33	1	Proton acceptor; specific for D-alanine By similarity
	Active site	236	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	33	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67687 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 376A3E0327E80752	FASTA	338	38,203
	10 20 30 40 50 60 MPRAILEINS ERIRHNVKKL SEFSGKKVIA VIKADAYGIG SIQMAKILEP IEEVDAFAVA 70 80 90 100 110 120 CVEEGVELRK MGIKKEILIL GGVLKDEVPL VEEYFLTPVV SDIEHLRAIG NRDIKFHVKY 130 140 150 160 170 180 DTGMGRLGFL NEVIHDPRVE GIMSHLSSPA DEEFSKLQIK KFEEIVKKYT KVEKIHMESS 190 200 210 220 230 240 AGVVYRVPFT THIRVGLAMY GEKPLKNYPV DIKPALTLKA KLISVKELPE NYPVSYSRTY 250 260 270 280 290 300 VTKKKTKTGV VAFGYADGLM KTLSNRSYLL YKGEKLPIFG NITMDMTIVD LKNTDAKVGD 310 320 330 WVYVVNEERT FTELAREAGT IPYELMCNLS KRIKRVVV

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07650.1.
PIR	E70457.
RefSeq	NP_214253.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67687.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193494.
GenomeReviews	Gene locus aq_1827 in contig AE000657_GR.
KEGG	aae:aq_1827.
NMPDR	fig|224324.1.peg.1268.
PATRIC	20960620. VBIAquAeo85532_1411.
Phylogenomic databases
HOGENOM	HBG712172.
OMA	SIQDYEY.
PhylomeDB	O67687.
ProtClustDB	CLSK2299837.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1827-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_AQUAE

Accession

Primary (citable) accession number: O67687

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program