Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei13S-adenosyl-L-methionine; via amide nitrogen1
Binding sitei38S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei60S-adenosyl-L-methionine1
Binding sitei83S-adenosyl-L-methionine1
Binding sitei101S-adenosyl-L-methionine1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.182. 396.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:aq_1816
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001014741 – 248Ribosomal RNA small subunit methyltransferase AAdd BLAST248

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei105Interaction with RNA1
Sitei198Interaction with RNA1
Sitei202Interaction with RNA1
Sitei204Interaction with RNA1

Protein-protein interaction databases

STRINGi224324.aq_1816.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 9Combined sources4
Helixi16 – 25Combined sources10
Beta strandi33 – 39Combined sources7
Helixi41 – 48Combined sources8
Beta strandi54 – 59Combined sources6
Helixi63 – 69Combined sources7
Beta strandi77 – 80Combined sources4
Turni84 – 86Combined sources3
Helixi89 – 91Combined sources3
Beta strandi94 – 101Combined sources8
Turni104 – 106Combined sources3
Helixi107 – 116Combined sources10
Helixi117 – 120Combined sources4
Beta strandi122 – 129Combined sources8
Helixi130 – 137Combined sources8
Beta strandi139 – 141Combined sources3
Helixi144 – 152Combined sources9
Beta strandi153 – 161Combined sources9
Helixi163 – 165Combined sources3
Beta strandi166 – 168Combined sources3
Beta strandi174 – 181Combined sources8
Helixi190 – 201Combined sources12
Turni202 – 205Combined sources4
Helixi208 – 210Combined sources3
Helixi214 – 219Combined sources6
Helixi228 – 230Combined sources3
Helixi233 – 244Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FTCX-ray1.68A1-248[»]
3FTDX-ray1.44A1-248[»]
3FTEX-ray3.00A1-248[»]
3FTFX-ray2.80A1-248[»]
3R9XX-ray2.80B1-248[»]
ProteinModelPortaliO67680.
SMRiO67680.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67680.

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227962.
InParanoidiO67680.
KOiK02528.
OMAiVKANWYA.
OrthoDBiPOG091H02E3.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRLKKSFGQ HLLVSEGVLK KIAEELNIEE GNTVVEVGGG TGNLTKVLLQ
60 70 80 90 100
HPLKKLYVIE LDREMVENLK SIGDERLEVI NEDASKFPFC SLGKELKVVG
110 120 130 140 150
NLPYNVASLI IENTVYNKDC VPLAVFMVQK EVAEKLQGKK DTGWLSVFVR
160 170 180 190 200
TFYDVNYVMT VPPRFFVPPP KVQSAVIKLV KNEKFPVKDL KNYKKFLTKI
210 220 230 240
FQNRRKVLRK KIPEELLKEA GINPDARVEQ LSLEDFFKLY RLIEDSGE
Length:248
Mass (Da):28,355
Last modified:August 1, 1998 - v1
Checksum:i259FA7AA81786AF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07637.1.
PIRiF70456.
RefSeqiNP_214246.1. NC_000918.1.
WP_010881183.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07637; AAC07637; aq_1816.
GeneIDi1193487.
KEGGiaae:aq_1816.
PATRICi20960604. VBIAquAeo85532_1403.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07637.1.
PIRiF70456.
RefSeqiNP_214246.1. NC_000918.1.
WP_010881183.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FTCX-ray1.68A1-248[»]
3FTDX-ray1.44A1-248[»]
3FTEX-ray3.00A1-248[»]
3FTFX-ray2.80A1-248[»]
3R9XX-ray2.80B1-248[»]
ProteinModelPortaliO67680.
SMRiO67680.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1816.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07637; AAC07637; aq_1816.
GeneIDi1193487.
KEGGiaae:aq_1816.
PATRICi20960604. VBIAquAeo85532_1403.

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227962.
InParanoidiO67680.
KOiK02528.
OMAiVKANWYA.
OrthoDBiPOG091H02E3.

Enzyme and pathway databases

BRENDAi2.1.1.182. 396.

Miscellaneous databases

EvolutionaryTraceiO67680.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMA_AQUAE
AccessioniPrimary (citable) accession number: O67680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.