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O67680

- RSMA_AQUAE

UniProt

O67680 - RSMA_AQUAE

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Protein
Ribosomal RNA small subunit methyltransferase A
Gene
rsmA, ksgA, aq_1816
Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei13 – 131S-adenosyl-L-methionine; via amide nitrogen
Binding sitei38 – 381S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei60 – 601S-adenosyl-L-methionine
Binding sitei83 – 831S-adenosyl-L-methionine
Binding sitei101 – 1011S-adenosyl-L-methionine
Sitei105 – 1051Interaction with RNA
Sitei198 – 1981Interaction with RNA
Sitei202 – 2021Interaction with RNA
Sitei204 – 2041Interaction with RNA

GO - Molecular functioni

  1. 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Source: UniProtKB-EC
  2. RNA binding Source: UniProtKB-KW
  3. rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1301-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase A (EC:2.1.1.182)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene namesi
Name:rsmA
Synonyms:ksgA
Ordered Locus Names:aq_1816
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Ribosomal RNA small subunit methyltransferase AUniRule annotation
PRO_0000101474Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_1816.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni6 – 94
Helixi16 – 2510
Beta strandi33 – 397
Helixi41 – 488
Beta strandi54 – 596
Helixi63 – 697
Beta strandi77 – 804
Turni84 – 863
Helixi89 – 913
Beta strandi94 – 1018
Turni104 – 1063
Helixi107 – 11610
Helixi117 – 1204
Beta strandi122 – 1298
Helixi130 – 1378
Beta strandi139 – 1413
Helixi144 – 1529
Beta strandi153 – 1619
Helixi163 – 1653
Beta strandi166 – 1683
Beta strandi174 – 1818
Helixi190 – 20112
Turni202 – 2054
Helixi208 – 2103
Helixi214 – 2196
Helixi228 – 2303
Helixi233 – 24412

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FTCX-ray1.68A1-248[»]
3FTDX-ray1.44A1-248[»]
3FTEX-ray3.00A1-248[»]
3FTFX-ray2.80A1-248[»]
3R9XX-ray2.80B1-248[»]
ProteinModelPortaliO67680.

Miscellaneous databases

EvolutionaryTraceiO67680.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0030.
HOGENOMiHOG000227962.
KOiK02528.
OMAiWRRALEC.
OrthoDBiEOG66F08Z.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67680-1 [UniParc]FASTAAdd to Basket

« Hide

MVRLKKSFGQ HLLVSEGVLK KIAEELNIEE GNTVVEVGGG TGNLTKVLLQ    50
HPLKKLYVIE LDREMVENLK SIGDERLEVI NEDASKFPFC SLGKELKVVG 100
NLPYNVASLI IENTVYNKDC VPLAVFMVQK EVAEKLQGKK DTGWLSVFVR 150
TFYDVNYVMT VPPRFFVPPP KVQSAVIKLV KNEKFPVKDL KNYKKFLTKI 200
FQNRRKVLRK KIPEELLKEA GINPDARVEQ LSLEDFFKLY RLIEDSGE 248
Length:248
Mass (Da):28,355
Last modified:August 1, 1998 - v1
Checksum:i259FA7AA81786AF8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07637.1.
PIRiF70456.
RefSeqiNP_214246.1. NC_000918.1.
WP_010881183.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07637; AAC07637; aq_1816.
GeneIDi1193487.
KEGGiaae:aq_1816.
PATRICi20960604. VBIAquAeo85532_1403.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07637.1 .
PIRi F70456.
RefSeqi NP_214246.1. NC_000918.1.
WP_010881183.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3FTC X-ray 1.68 A 1-248 [» ]
3FTD X-ray 1.44 A 1-248 [» ]
3FTE X-ray 3.00 A 1-248 [» ]
3FTF X-ray 2.80 A 1-248 [» ]
3R9X X-ray 2.80 B 1-248 [» ]
ProteinModelPortali O67680.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_1816.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07637 ; AAC07637 ; aq_1816 .
GeneIDi 1193487.
KEGGi aae:aq_1816.
PATRICi 20960604. VBIAquAeo85532_1403.

Phylogenomic databases

eggNOGi COG0030.
HOGENOMi HOG000227962.
KOi K02528.
OMAi WRRALEC.
OrthoDBi EOG66F08Z.

Enzyme and pathway databases

BioCyci AAEO224324:GJBH-1301-MONOMER.

Miscellaneous databases

EvolutionaryTracei O67680.

Family and domain databases

Gene3Di 1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPi MF_00607. 16SrRNA_methyltr_A.
InterProi IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR11727. PTHR11727. 1 hit.
Pfami PF00398. RrnaAD. 1 hit.
[Graphical view ]
SMARTi SM00650. rADc. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00755. ksgA. 1 hit.
PROSITEi PS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.
  2. "Structural basis for binding of RNA and cofactor by a KsgA methyltransferase."
    Tu C., Tropea J.E., Austin B.P., Court D.L., Waugh D.S., Ji X.
    Structure 17:374-385(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) IN COMPLEXES WITH RNA AND S-ADENOSYL-L-HOMOCYSTEINE, RNA-BINDING.

Entry informationi

Entry nameiRSMA_AQUAE
AccessioniPrimary (citable) accession number: O67680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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