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O67680

- RSMA_AQUAE

UniProt

O67680 - RSMA_AQUAE

Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    • Comment

    Functioni

    Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

    Catalytic activityi

    4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei13 – 131S-adenosyl-L-methionine; via amide nitrogen
    Binding sitei38 – 381S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei60 – 601S-adenosyl-L-methionine
    Binding sitei83 – 831S-adenosyl-L-methionine
    Binding sitei101 – 1011S-adenosyl-L-methionine
    Sitei105 – 1051Interaction with RNA
    Sitei198 – 1981Interaction with RNA
    Sitei202 – 2021Interaction with RNA
    Sitei204 – 2041Interaction with RNA

    GO - Molecular functioni

    1. 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Source: UniProtKB-EC
    2. RNA binding Source: UniProtKB-KW
    3. rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    rRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1301-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)
    Alternative name(s):
    16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
    16S rRNA dimethyladenosine transferaseUniRule annotation
    16S rRNA dimethylaseUniRule annotation
    S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
    Gene namesi
    Name:rsmAUniRule annotation
    Synonyms:ksgAUniRule annotation
    Ordered Locus Names:aq_1816
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 248248Ribosomal RNA small subunit methyltransferase APRO_0000101474Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi224324.aq_1816.

    Structurei

    Secondary structure

    1
    248
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni6 – 94
    Helixi16 – 2510
    Beta strandi33 – 397
    Helixi41 – 488
    Beta strandi54 – 596
    Helixi63 – 697
    Beta strandi77 – 804
    Turni84 – 863
    Helixi89 – 913
    Beta strandi94 – 1018
    Turni104 – 1063
    Helixi107 – 11610
    Helixi117 – 1204
    Beta strandi122 – 1298
    Helixi130 – 1378
    Beta strandi139 – 1413
    Helixi144 – 1529
    Beta strandi153 – 1619
    Helixi163 – 1653
    Beta strandi166 – 1683
    Beta strandi174 – 1818
    Helixi190 – 20112
    Turni202 – 2054
    Helixi208 – 2103
    Helixi214 – 2196
    Helixi228 – 2303
    Helixi233 – 24412

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3FTCX-ray1.68A1-248[»]
    3FTDX-ray1.44A1-248[»]
    3FTEX-ray3.00A1-248[»]
    3FTFX-ray2.80A1-248[»]
    3R9XX-ray2.80B1-248[»]
    ProteinModelPortaliO67680.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO67680.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0030.
    HOGENOMiHOG000227962.
    KOiK02528.
    OMAiWRRALEC.
    OrthoDBiEOG66F08Z.

    Family and domain databases

    Gene3Di1.10.8.100. 1 hit.
    3.40.50.150. 1 hit.
    HAMAPiMF_00607. 16SrRNA_methyltr_A.
    InterProiIPR023165. rRNA_Ade_diMease-like.
    IPR020596. rRNA_Ade_Mease_Trfase_CS.
    IPR001737. rRNA_Ade_methylase_transferase.
    IPR020598. rRNA_Ade_methylase_Trfase_N.
    IPR011530. rRNA_adenine_dimethylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR11727. PTHR11727. 1 hit.
    PfamiPF00398. RrnaAD. 1 hit.
    [Graphical view]
    SMARTiSM00650. rADc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00755. ksgA. 1 hit.
    PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
    PS51689. SAM_RNA_A_N6_MT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O67680-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVRLKKSFGQ HLLVSEGVLK KIAEELNIEE GNTVVEVGGG TGNLTKVLLQ    50
    HPLKKLYVIE LDREMVENLK SIGDERLEVI NEDASKFPFC SLGKELKVVG 100
    NLPYNVASLI IENTVYNKDC VPLAVFMVQK EVAEKLQGKK DTGWLSVFVR 150
    TFYDVNYVMT VPPRFFVPPP KVQSAVIKLV KNEKFPVKDL KNYKKFLTKI 200
    FQNRRKVLRK KIPEELLKEA GINPDARVEQ LSLEDFFKLY RLIEDSGE 248
    Length:248
    Mass (Da):28,355
    Last modified:August 1, 1998 - v1
    Checksum:i259FA7AA81786AF8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07637.1.
    PIRiF70456.
    RefSeqiNP_214246.1. NC_000918.1.
    WP_010881183.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07637; AAC07637; aq_1816.
    GeneIDi1193487.
    KEGGiaae:aq_1816.
    PATRICi20960604. VBIAquAeo85532_1403.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07637.1 .
    PIRi F70456.
    RefSeqi NP_214246.1. NC_000918.1.
    WP_010881183.1. NC_000918.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3FTC X-ray 1.68 A 1-248 [» ]
    3FTD X-ray 1.44 A 1-248 [» ]
    3FTE X-ray 3.00 A 1-248 [» ]
    3FTF X-ray 2.80 A 1-248 [» ]
    3R9X X-ray 2.80 B 1-248 [» ]
    ProteinModelPortali O67680.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_1816.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07637 ; AAC07637 ; aq_1816 .
    GeneIDi 1193487.
    KEGGi aae:aq_1816.
    PATRICi 20960604. VBIAquAeo85532_1403.

    Phylogenomic databases

    eggNOGi COG0030.
    HOGENOMi HOG000227962.
    KOi K02528.
    OMAi WRRALEC.
    OrthoDBi EOG66F08Z.

    Enzyme and pathway databases

    BioCyci AAEO224324:GJBH-1301-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O67680.

    Family and domain databases

    Gene3Di 1.10.8.100. 1 hit.
    3.40.50.150. 1 hit.
    HAMAPi MF_00607. 16SrRNA_methyltr_A.
    InterProi IPR023165. rRNA_Ade_diMease-like.
    IPR020596. rRNA_Ade_Mease_Trfase_CS.
    IPR001737. rRNA_Ade_methylase_transferase.
    IPR020598. rRNA_Ade_methylase_Trfase_N.
    IPR011530. rRNA_adenine_dimethylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR11727. PTHR11727. 1 hit.
    Pfami PF00398. RrnaAD. 1 hit.
    [Graphical view ]
    SMARTi SM00650. rADc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00755. ksgA. 1 hit.
    PROSITEi PS01131. RRNA_A_DIMETH. 1 hit.
    PS51689. SAM_RNA_A_N6_MT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.
    2. "Structural basis for binding of RNA and cofactor by a KsgA methyltransferase."
      Tu C., Tropea J.E., Austin B.P., Court D.L., Waugh D.S., Ji X.
      Structure 17:374-385(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) IN COMPLEXES WITH RNA AND S-ADENOSYL-L-HOMOCYSTEINE, RNA-BINDING.

    Entry informationi

    Entry nameiRSMA_AQUAE
    AccessioniPrimary (citable) accession number: O67680
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 13, 2002
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3