Malate dehydrogenase 1 - Aquifex aeolicus

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Reviewed, UniProtKB/Swiss-Prot O67655 (MDH1_AQUAE)

Last modified November 25, 2008. Version 67. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Malate dehydrogenase 1
EC=1.1.1.37

Gene names

Name:	mdh1
Synonyms:	mdh
Ordered Locus Names:	aq_1782

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD(+) = oxaloacetate + NADH.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

335

Malate dehydrogenase 1

PRO_0000113422

Regions

Nucleotide binding

6

NAD By similarity

Nucleotide binding

3

NAD By similarity

Sites

Active site

1

Proton acceptor By similarity

Binding site

1

NAD By similarity

Binding site

1

Substrate By similarity

Binding site

1

Substrate By similarity

Binding site

1

NAD By similarity

Binding site

1

Substrate By similarity

Binding site

1

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O67655-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: E02FA4C339237118
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335

36,675

        10         20         30         40         50         60 
MRMKKTVAVI GAGNVGEHVA SLILLKNLAN VKMFDLPRKT EEKVFEPVKG KALDMKQMLA 

        70         80         90        100        110        120 
AMDIDARVEG YTVTPEGEGY EPLEGSDIVV ITAGFPRRPG MSREDLLEAN IRIISVIADR 

       130        140        150        160        170        180 
IKRYAPDAIV IVVTNPVDVM TYVAYKLLNF PKNRVMGMAG VLDSARFKTF ISEELMVSPK 

       190        200        210        220        230        240 
DIHAYVIGGH GDEMVPLISI SNVGGIPLKD LLPKEKLEKI IERTRFGGGE IVNLMGTSAY 

       250        260        270        280        290        300 
YAPAAAIVDM IEALVQNSKR ILPCSVYLDG EAGEYYGVQG FCVGVPVKLG SNGVEEIIKV 

       310        320        330 
PMIEEEREMW RRSVESVKKT VEVAEGILSA GSSRQ

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed: 9537320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases
	AE000657 Genomic DNA. Translation: AAC07619.1.
PIR	E70453.
RefSeq	NP_214221.1.
3D structure databases
HSSP	HSSP built from PDB template 1GV0 based on UniProtKB P80039.
ModBase	Search...
Genome annotation databases
GeneID	1193462.
GenomeReviews	Gene locus aq_1782 in contig AE000657_GR.
KEGG	aae:aq_1782.
NMPDR	fig\|224324.1.peg.1236.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67655.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1782-MON.
Family and domain databases
HAMAP	MF_00487. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR011275. Malate_DHase_NAD-dep_bac. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH1_AQUAE

Accession

Primary (citable) accession number: O67655

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents