Reviewed, UniProtKB/Swiss-Prot O67648 (LPXC_AQUAE)

Names and origin

Protein names

Recommended name:
    UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase
    EC=3.5.1.-
Alternative name(s):
    UDP-3-O-acyl-GlcNAc deacetylase

Gene names

Name:	lpxC
Synonyms:	envA
Ordered Locus Names:	aq_1772

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity.
Catalytic activity	UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine + H2O = UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine + acetate. HAMAP MF_00388
Pathway	Glycolipid biosynthesis; lipid (IV)A biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-D-glucosamine: step 2/6. HAMAP MF_00388
Sequence similarities	Belongs to the lpxC family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 282

282

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase HAMAP MF_00388

PRO_0000191917

Secondary structure

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282

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Beta strand

11 – 16

6

Turn

18 – 20

3

Beta strand

23 – 30

8

Beta strand

37 – 41

5

Beta strand

44 – 49

6

Helix

50 – 52

3

Beta strand

53 – 55

3

Beta strand

57 – 59

3

Beta strand

61 – 64

4

Beta strand

67 – 70

4

Helix

73 – 81

9

Beta strand

86 – 95

10

Beta strand

101 – 103

3

Helix

104 – 111

8

Beta strand

114 – 120

7

Beta strand

130 – 134

5

Beta strand

137 – 142

6

Beta strand

148 – 154

7

Beta strand

161 – 167

7

Helix

171 – 173

3

Turn

174 – 176

3

Beta strand

180 – 182

3

Helix

183 – 185

3

Helix

186 – 191

6

Turn

200 – 202

3

Beta strand

204 – 206

3

Helix

222 – 235

14

Helix

236 – 238

3

Beta strand

245 – 250

6

Helix

253 – 265

13

Sequences

	Sequence		Length	Mass (Da)
	O67648-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 38EFE076144B5F27	FASTA	282	32,145
	10 20 30 40 50 60 MGLEKTVKEK LSFEGVGIHT GEYSKLIIHP EKEGTGIRFF KNGVYIPARH EFVVHTNHST 70 80 90 100 110 120 DLGFKGQRIK TVEHILSVLH LLEITNVTIE VIGNEIPILD GSGWEFYEAI RKNILNQNRE 130 140 150 160 170 180 IDYFVVEEPI IVEDEGRLIK AEPSDTLEVT YEGEFKNFLG RQKFTFVEGN EEEIVLARTF 190 200 210 220 230 240 CFDWEIEHIK KVGLGKGGSL KNTLVLGKDK VYNPEGLRYE NEPVRHKVFD LIGDLYLLGS 250 260 270 280 PVKGKFYSFR GGHSLNVKLV KELAKKQKLT RDLPHLPSVQ AL

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000657 Genomic DNA. Translation: AAC07605.1.

PIR

F70452.

RefSeq

NP_214214.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P42	X-ray	2.00	A/B	2-271	[»]
1XXE	NMR	-	A	1-282	[»]
1YH8	X-ray	2.70	A/B	2-271	[»]
1YHC	X-ray	2.10	A/B	2-271	[»]
2GO3	X-ray	2.00	A/B	1-255	[»]
2GO4	X-ray	2.70	A/B	1-267	[»]
2IER	X-ray	2.70	A/B	1-271	[»]
2IES	X-ray	3.10	A/B	1-271	[»]
2J65	X-ray	2.20	A/B	1-271	[»]
2JT2	NMR	-	A	1-274	[»]
2O3Z	X-ray	2.25	A/B	1-271	[»]

ModBase

Search...

Genome annotation databases

GeneID

1193363.

GenomeReviews

Gene locus aq_1772 in contig AE000657_GR.

KEGG

aae:aq_1772.

NMPDR

fig|224324.1.peg.1229.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

O67648.

OMA

O67648. TGVGVHS.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_1772-MON.

Family and domain databases

HAMAP

MF_00388.
[Tree]

InterPro

IPR004463. UDP-acyl_GlcNac_deAcase.
IPR011334. UDP-acyl_GlcNac_deAcase_C.
IPR015870. UDP-acyl_N-AcGlcN_deAcase_N.
[Graphical view]

Gene3D

G3DSA:3.30.1700.10. UDP-acyl_N-AcGlcN_deAcase_C. 1 hit.
G3DSA:3.30.230.20. UDP-acyl_N-AcGlcN_deAcase_N. 1 hit.

Pfam

PF03331. LpxC. 1 hit.
[Graphical view]

ProDom

PD011499. Lipid_A_LpxC. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00325. lpxC. 1 hit.

ProtoNet

Search...

Entry information

Entry name

LPXC_AQUAE

Accession

Primary (citable) accession number: O67648

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)