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O67646 (SYLB_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase subunit beta

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase subunit beta
Short name=LeuRS
Gene names
Name:leuS'
Ordered Locus Names:aq_1770
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length289 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Subunit structure

Seems to consist of an alpha chain and a beta chain.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 289289Leucine--tRNA ligase subunit beta
PRO_0000151963

Regions

Motif45 – 495"KMSKS" region

Sites

Binding site481ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O67646 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 585B825AC6E3F606

FASTA28933,536
        10         20         30         40         50         60 
MKIKDFLQEN KISVGDNAIF LLEKLGIKDE NLIKFLEREI GESAKMSKSK ANVVDPEEAV 

        70         80         90        100        110        120 
EKYGADTVRL YILFAAPPEQ DFEWTDEGIQ GAYRFLQRYW NFVNKHLEKI KNLTYTVEEL 

       130        140        150        160        170        180 
RNVQGKAKEV RREIHQTIAD YRRDFEERYQ FNTAIAKIMK LLNTLQDFSP QTEQDYKVLR 

       190        200        210        220        230        240 
EGIETITLLL SPITPHIAEE VWEMLGNEGF IINQPIPEPD PEALKVEEIE IPVQVNGKLR 

       250        260        270        280 
ARVKVPADAD EETVKNIVLS DERVQKWVQG KEVKKFIYVK GKLVNVVVK 

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC07608.1.
PIRD70452.
RefSeqNP_214212.1. NC_000918.1.

3D structure databases

ProteinModelPortalO67646.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_1770.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC07608; AAC07608; aq_1770.
GeneID1193361.
KEGGaae:aq_1770.
PATRIC20960530. VBIAquAeo85532_1366.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000248928.
KOK01869.
OMASKSKHNT.
OrthoDBEOG6SZ1G3.
ProtClustDBCLSK692031.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-1267-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 1 hit.
InterProIPR002302. Leu-tRNA-ligase_bac/mito.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYLB_AQUAE
AccessionPrimary (citable) accession number: O67646
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries