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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei228ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-1246-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:aq_1751
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000556401 – 392Tyrosine--tRNA ligaseAdd BLAST392

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_1751

Structurei

3D structure databases

ProteinModelPortaliO67632
SMRiO67632
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini330 – 390S4 RNA-bindingUniRule annotationAdd BLAST61

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 50"HIGH" region10
Motifi225 – 229"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DA0 Bacteria
COG0162 LUCA
HOGENOMiHOG000242791
InParanoidiO67632
KOiK01866
OMAiVNYMMAK
OrthoDBiPOG091H02CB

Family and domain databases

CDDicd00165 S4, 1 hit
cd00805 TyrRS_core, 1 hit
Gene3Di3.10.290.10, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_02007 Tyr_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002942 S4_RNA-bd
IPR036986 S4_RNA-bd_sf
IPR002307 Tyr-tRNA-ligase
IPR024088 Tyr-tRNA-ligase_bac-type
IPR024108 Tyr-tRNA-ligase_bac_2
PANTHERiPTHR11766 PTHR11766, 1 hit
PTHR11766:SF1 PTHR11766:SF1, 1 hit
PfamiView protein in Pfam
PF01479 S4, 1 hit
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01040 TRNASYNTHTYR
SMARTiView protein in SMART
SM00363 S4, 1 hit
TIGRFAMsiTIGR00234 tyrS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
PS50889 S4, 1 hit

Sequencei

Sequence statusi: Complete.

O67632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPEEQLRII KEGTVEIIEE EELLKKLKEG RPLRVKAGFD PTAPDLHLGH
60 70 80 90 100
VVLLQKLRQF QQLGHEVFFI IGDFTAMIGD PTGRSQTRPP LSREQVLENA
110 120 130 140 150
KTYEHQVFKV LIPEKTTVVF NSTWLEELGT KGLIELCAKY TVARMLERED
160 170 180 190 200
FSKRFKEGIP IYIHEFIYPL LQAYDSVAIK ADVEIGGTDQ KFNLLIGRDI
210 220 230 240 250
QREYGQEPQV CITLPLLVGT DGVRKMSKSY GNYVGITEDP KTMFAKIMSI
260 270 280 290 300
PDEIMWDWFL LLTDYNKEEI EKMRREMHPM EAKKLLAFTI VKRFHSEEEA
310 320 330 340 350
RKAKEWWEKT FSQREFPEDA PLVKLNEKKL RAVDFLVKIG AVKSKNEARR
360 370 380 390
VIQGGGLKIN GEKVTDPNTE IEINGELKVK VGKKKFYRVV SG
Length:392
Mass (Da):45,200
Last modified:August 1, 1998 - v1
Checksum:i7B160BEF801665B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC07595.1
PIRiF70450
RefSeqiNP_214198.1, NC_000918.1
WP_010881135.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC07595; AAC07595; aq_1751
GeneIDi1193347
KEGGiaae:aq_1751
PATRICifig|224324.8.peg.1350

Similar proteinsi

Entry informationi

Entry nameiSYY_AQUAE
AccessioniPrimary (citable) accession number: O67632
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health