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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:aq_1751
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Tyrosine--tRNA ligasePRO_0000055640Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_1751.

Structurei

3D structure databases

ProteinModelPortaliO67632.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini330 – 39061S4 RNA-bindingUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5010"HIGH" region
Motifi225 – 2295"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily.UniRule annotation
Contains 1 S4 RNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DA0. Bacteria.
COG0162. LUCA.
HOGENOMiHOG000242791.
InParanoidiO67632.
KOiK01866.
OMAiDMFGKVM.
OrthoDBiEOG6B09VR.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02007. Tyr_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024108. Tyr-tRNA-ligase_bac_2.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPEEQLRII KEGTVEIIEE EELLKKLKEG RPLRVKAGFD PTAPDLHLGH
60 70 80 90 100
VVLLQKLRQF QQLGHEVFFI IGDFTAMIGD PTGRSQTRPP LSREQVLENA
110 120 130 140 150
KTYEHQVFKV LIPEKTTVVF NSTWLEELGT KGLIELCAKY TVARMLERED
160 170 180 190 200
FSKRFKEGIP IYIHEFIYPL LQAYDSVAIK ADVEIGGTDQ KFNLLIGRDI
210 220 230 240 250
QREYGQEPQV CITLPLLVGT DGVRKMSKSY GNYVGITEDP KTMFAKIMSI
260 270 280 290 300
PDEIMWDWFL LLTDYNKEEI EKMRREMHPM EAKKLLAFTI VKRFHSEEEA
310 320 330 340 350
RKAKEWWEKT FSQREFPEDA PLVKLNEKKL RAVDFLVKIG AVKSKNEARR
360 370 380 390
VIQGGGLKIN GEKVTDPNTE IEINGELKVK VGKKKFYRVV SG
Length:392
Mass (Da):45,200
Last modified:August 1, 1998 - v1
Checksum:i7B160BEF801665B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07595.1.
PIRiF70450.
RefSeqiNP_214198.1. NC_000918.1.
WP_010881135.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07595; AAC07595; aq_1751.
GeneIDi1193347.
KEGGiaae:aq_1751.
PATRICi20960492. VBIAquAeo85532_1350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07595.1.
PIRiF70450.
RefSeqiNP_214198.1. NC_000918.1.
WP_010881135.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67632.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1751.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07595; AAC07595; aq_1751.
GeneIDi1193347.
KEGGiaae:aq_1751.
PATRICi20960492. VBIAquAeo85532_1350.

Phylogenomic databases

eggNOGiENOG4105DA0. Bacteria.
COG0162. LUCA.
HOGENOMiHOG000242791.
InParanoidiO67632.
KOiK01866.
OMAiDMFGKVM.
OrthoDBiEOG6B09VR.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1250-MONOMER.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02007. Tyr_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024108. Tyr-tRNA-ligase_bac_2.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiSYY_AQUAE
AccessioniPrimary (citable) accession number: O67632
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 17, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.