O67632 (SYY_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	aq_1751

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02007
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity. HAMAP MF_02007
Subcellular location	Cytoplasm By similarity HAMAP MF_02007.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Tyrosine--tRNA ligase HAMAP MF_02007		PRO_0000055640
Regions
	Domain	330 – 390	61	S4 RNA-binding
	Motif	41 – 50	10	"HIGH" region HAMAP MF_02007
	Motif	225 – 229	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	228	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67632 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7B160BEF801665B2	FASTA	392	45,200
	10 20 30 40 50 60 MTPEEQLRII KEGTVEIIEE EELLKKLKEG RPLRVKAGFD PTAPDLHLGH VVLLQKLRQF 70 80 90 100 110 120 QQLGHEVFFI IGDFTAMIGD PTGRSQTRPP LSREQVLENA KTYEHQVFKV LIPEKTTVVF 130 140 150 160 170 180 NSTWLEELGT KGLIELCAKY TVARMLERED FSKRFKEGIP IYIHEFIYPL LQAYDSVAIK 190 200 210 220 230 240 ADVEIGGTDQ KFNLLIGRDI QREYGQEPQV CITLPLLVGT DGVRKMSKSY GNYVGITEDP 250 260 270 280 290 300 KTMFAKIMSI PDEIMWDWFL LLTDYNKEEI EKMRREMHPM EAKKLLAFTI VKRFHSEEEA 310 320 330 340 350 360 RKAKEWWEKT FSQREFPEDA PLVKLNEKKL RAVDFLVKIG AVKSKNEARR VIQGGGLKIN 370 380 390 GEKVTDPNTE IEINGELKVK VGKKKFYRVV SG

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07595.1.
PIR	F70450.
RefSeq	NP_214198.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67632.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193347.
GenomeReviews	Gene locus aq_1751 in contig AE000657_GR.
KEGG	aae:aq_1751.
NMPDR	fig|224324.1.peg.1213.
PATRIC	20960492. VBIAquAeo85532_1350.
Phylogenomic databases
HOGENOM	HBG288125.
OMA	IPTYFEL.
PhylomeDB	O67632.
ProtClustDB	PRK05912.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1751-MONOMER.
Family and domain databases
HAMAP	MF_02007. Tyr_tRNA_synth_type2. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024108. Tyr-tRNA-synth_bac_2. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_AQUAE

Accession

Primary (citable) accession number: O67632

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program