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Protein

Elongation factor 4

Gene

lepA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 21GTPUniRule annotation1 Publication6
Nucleotide bindingi133 – 136GTPUniRule annotation1 Publication4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:aq_1725
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001762231 – 600Elongation factor 4Add BLAST600

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_1725.

Structurei

Secondary structure

1600
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 5Combined sources3
Beta strandi6 – 12Combined sources7
Helixi19 – 30Combined sources12
Beta strandi60 – 66Combined sources7
Beta strandi72 – 78Combined sources7
Helixi84 – 86Combined sources3
Helixi87 – 95Combined sources9
Beta strandi98 – 105Combined sources8
Turni106 – 108Combined sources3
Helixi112 – 123Combined sources12
Beta strandi127 – 133Combined sources7
Beta strandi137 – 139Combined sources3
Helixi142 – 151Combined sources10
Helixi157 – 159Combined sources3
Beta strandi161 – 164Combined sources4
Turni165 – 168Combined sources4
Helixi171 – 181Combined sources11
Beta strandi194 – 203Combined sources10
Turni204 – 206Combined sources3
Beta strandi207 – 219Combined sources13
Beta strandi224 – 227Combined sources4
Turni228 – 230Combined sources3
Beta strandi233 – 235Combined sources3
Beta strandi238 – 250Combined sources13
Beta strandi257 – 263Combined sources7
Helixi267 – 269Combined sources3
Beta strandi275 – 281Combined sources7
Beta strandi297 – 303Combined sources7
Helixi309 – 320Combined sources12
Beta strandi327 – 333Combined sources7
Turni334 – 336Combined sources3
Beta strandi337 – 346Combined sources10
Helixi347 – 361Combined sources15
Beta strandi365 – 367Combined sources3
Beta strandi374 – 378Combined sources5
Beta strandi385 – 389Combined sources5
Helixi390 – 392Combined sources3
Helixi397 – 399Combined sources3
Beta strandi400 – 415Combined sources16
Helixi416 – 418Combined sources3
Helixi419 – 428Combined sources10
Beta strandi432 – 440Combined sources9
Beta strandi443 – 451Combined sources9
Helixi452 – 466Combined sources15
Turni467 – 469Combined sources3
Beta strandi472 – 482Combined sources11
Beta strandi485 – 496Combined sources12
Helixi498 – 500Combined sources3
Beta strandi502 – 505Combined sources4
Helixi506 – 508Combined sources3
Helixi509 – 523Combined sources15
Beta strandi531 – 537Combined sources7
Beta strandi540 – 547Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YWEX-ray2.05A1-600[»]
2YWFX-ray2.24A1-600[»]
2YWGX-ray2.94A1-600[»]
2YWHX-ray2.24A1-600[»]
ProteinModelPortaliO67618.
SMRiO67618.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67618.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 186tr-type GAdd BLAST183

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
HOGENOMiHOG000020624.
InParanoidiO67618.
KOiK03596.
OMAiFAGIYPV.
OrthoDBiPOG091H02HW.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA. 1 hit.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQKNVRNFC IIAHVDHGKS TLADRLLEYT GAISEREKRE QLLDTLDVER
60 70 80 90 100
ERGITVKMQA VRMFYKAKDG NTYKLHLIDT PGHVDFSYEV SRALAACEGA
110 120 130 140 150
LLLIDASQGI EAQTVANFWK AVEQDLVIIP VINKIDLPSA DVDRVKKQIE
160 170 180 190 200
EVLGLDPEEA ILASAKEGIG IEEILEAIVN RIPPPKGDPQ KPLKALIFDS
210 220 230 240 250
YYDPYRGAVA FVRIFDGEVK PGDKIMLMST GKEYEVTEVG AQTPKMTKFD
260 270 280 290 300
KLSAGDVGYI AASIKDVRDI RIGDTITHAK NPTKEPVPGF QPAKPMVYAG
310 320 330 340 350
IYPAEDTTYE ELRDALEKYA INDAAIVYEP ESSPALGMGF RVGFLGLLHM
360 370 380 390 400
EIVQERLERE YGVKIITTAP NVIYRVKKKF TDEVIEVRNP MDFPDNAGLI
410 420 430 440 450
EYVEEPFVLV TIITPKEYVG PIIQLCQEKR GIQKNMTYLD PNTVYLEYEM
460 470 480 490 500
PLSEIIVDFH DKIKSISRGF ASYDYEFIGY RPSDLIKLTV LINKKPVDAL
510 520 530 540 550
SFIVHADRAQ KFARRVAEKL RETIPRQLFE VHIQVAKGGK VIASERIKPL
560 570 580 590 600
RANVTAKCYG GDVTRKKKLL ENQKEGKKRM KQFGKVQLPQ EAFLSVLKVE
Length:600
Mass (Da):67,657
Last modified:August 1, 1998 - v1
Checksum:iEA69B32A062D3081
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07583.1.
PIRiH70448.
RefSeqiNP_214184.1. NC_000918.1.
WP_010881121.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07583; AAC07583; aq_1725.
GeneIDi1193333.
KEGGiaae:aq_1725.
PATRICi20960442. VBIAquAeo85532_1326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07583.1.
PIRiH70448.
RefSeqiNP_214184.1. NC_000918.1.
WP_010881121.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YWEX-ray2.05A1-600[»]
2YWFX-ray2.24A1-600[»]
2YWGX-ray2.94A1-600[»]
2YWHX-ray2.24A1-600[»]
ProteinModelPortaliO67618.
SMRiO67618.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1725.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07583; AAC07583; aq_1725.
GeneIDi1193333.
KEGGiaae:aq_1725.
PATRICi20960442. VBIAquAeo85532_1326.

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
HOGENOMiHOG000020624.
InParanoidiO67618.
KOiK03596.
OMAiFAGIYPV.
OrthoDBiPOG091H02HW.

Miscellaneous databases

EvolutionaryTraceiO67618.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA. 1 hit.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEPA_AQUAE
AccessioniPrimary (citable) accession number: O67618
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.