O67606 (METE_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	aq_1710

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	761 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 761	761	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098608
Sites
	Metal binding	643	1	Zinc By similarity
	Metal binding	645	1	Zinc By similarity
	Metal binding	729	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67606 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C8F69759F86D5383	FASTA	761	88,632
	10 20 30 40 50 60 MVKTYAFGFP KLGEKREFKR LLENYWKGKI SQEELIKGME ELNLWRANLY RENVEIIPSN 70 80 90 100 110 120 ELSYYDFMLD TTVMVGAIPS RFGEYRGLDT YFEMARGKNA LELTKWFNTN YHYLVPELEG 130 140 150 160 170 180 ENFKLLINRP LEDYKFFKEK GINTVPHVIG LFTFLKLSKA PKRVEEEGLP VIILEKLDTK 190 200 210 220 230 240 ERLEKFTKAL LPVYKELLES LKNEGVNRVH FEEPALVTDL EDYDWEILNE IYSELSKVGV 250 260 270 280 290 300 DLGILTYYDS VSDYDKFVNL PVKALHFDLV SNEENLKNIK EKGFPEDKEL IAGIINGRQP 310 320 330 340 350 360 WRANLREKVK LLEELLKVSA NLVVSNSCPL YHLPVTVEPE DELPEGLKER LSFAKEKLRE 370 380 390 400 410 420 IRLIKEALEG KEEALREVES SEKVMTAVFA QNPEVRRRLA SLSEKDFQRE LPYKERDKIQ 430 440 450 460 470 480 RDILKLPLFP TTTIGSFPQT EDVRKMRTAY RTGKISEEEY KNFIKQKIKY AIEVQEDIGL 490 500 510 520 530 540 DVLVHGEFER SDMVEYFAIK LDGIATTKHG WVLSYGSRVY RPPIIYGDVS RPAPMTLEEI 550 560 570 580 590 600 TYAQSLTNKP VKGMLTGPVT ILNWSYYRED VPKREIAYQI ALALLDEVKD LENAGIKVIQ 610 620 630 640 650 660 IDEPAFREGA PLKKRDWPEY FDWAVKAFRL ASRAKPETQI HTHMCYSEFN EIIEYIYQMD 670 680 690 700 710 720 FDVISIEASR SKGEIIEVFE KFKGWDRQIG IGVYDIHSPA IPTKEEMKAI MLRAMKVLPK 730 740 750 760 ELLWVNPDCG LKTRRWEEVI PALRNMVDMA KELREEVGVK V

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07565.1.
PIR	D70447.
RefSeq	NP_214172.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67606.
SMR	O67606. Positions 3-756.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193321.
GenomeReviews	Gene locus aq_1710 in contig AE000657_GR.
KEGG	aae:aq_1710.
NMPDR	fig|224324.1.peg.1187.
PATRIC	20960416. VBIAquAeo85532_1313.
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	O67606.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1710-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_AQUAE

Accession

Primary (citable) accession number: O67606

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program