Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O67605

- PFKA_AQUAE

UniProt

O67605 - PFKA_AQUAE

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101ATP; via amide nitrogenUniRule annotation
    Metal bindingi102 – 1021Magnesium; catalyticUniRule annotation
    Active sitei127 – 1271Proton acceptorUniRule annotation
    Binding sitei154 – 1541Allosteric activator ADPUniRule annotation
    Binding sitei162 – 1621Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei222 – 2221SubstrateUniRule annotation
    Binding sitei246 – 2461Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi71 – 722ATPUniRule annotation
    Nucleotide bindingi101 – 1044ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1222-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:aq_1708
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 321321ATP-dependent 6-phosphofructokinasePRO_0000111931Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224324.aq_1708.

    Structurei

    3D structure databases

    ProteinModelPortaliO67605.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni20 – 245Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni125 – 1273Substrate bindingUniRule annotation
    Regioni169 – 1713Substrate bindingUniRule annotation
    Regioni185 – 1873Allosteric activator ADP bindingUniRule annotation
    Regioni213 – 2153Allosteric activator ADP bindingUniRule annotation
    Regioni252 – 2554Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiAKLERGH.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O67605-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIGILTAGG DAPGMNAVIR AVVRSAEREG HEVIGIKRGY KGLIEGDFIK    50
    LTSRDVGGIL DRGGTILLSS RDPRYKKLEY REKAYKNLNE QGIEALIILG 100
    GEGTLAGAKL TAEETGIPVI GVPCTIDNDV GGTEYCIGYD TALRNAVDAI 150
    DKIRDTASSH ERIFIVEVMG RNRGFIAVEA GLATGAELIL IPEERFPREQ 200
    IPEEIEKAKK LGKLHFIIVL AEGYCRAKEL EDFLLEKIGD KYGEIRSTVL 250
    GHIQRGGIPT HFDRIMGTKF GVVAYESLIA GERSGFVAYE KGKFYIEDFE 300
    KAQEYKPIDR EAYKLNTRLA I 321
    Length:321
    Mass (Da):35,409
    Last modified:August 1, 1998 - v1
    Checksum:i23C4602AAFAFF491
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07573.1.
    PIRiC70447.
    RefSeqiNP_214171.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07573; AAC07573; aq_1708.
    GeneIDi1193320.
    KEGGiaae:aq_1708.
    PATRICi20960414. VBIAquAeo85532_1312.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07573.1 .
    PIRi C70447.
    RefSeqi NP_214171.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O67605.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_1708.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07573 ; AAC07573 ; aq_1708 .
    GeneIDi 1193320.
    KEGGi aae:aq_1708.
    PATRICi 20960414. VBIAquAeo85532_1312.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi AKLERGH.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci AAEO224324:GJBH-1222-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiPFKA_AQUAE
    AccessioniPrimary (citable) accession number: O67605
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3