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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10ATP; via amide nitrogenUniRule annotation1
Metal bindingi102Magnesium; catalyticUniRule annotation1
Active sitei127Proton acceptorUniRule annotation1
Binding sitei154Allosteric activator ADPUniRule annotation1
Binding sitei162Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei222SubstrateUniRule annotation1
Binding sitei246Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi71 – 72ATPUniRule annotation2
Nucleotide bindingi101 – 104ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-1216-MONOMER
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:aq_1708
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119311 – 321ATP-dependent 6-phosphofructokinaseAdd BLAST321

Proteomic databases

PRIDEiO67605

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_1708

Structurei

3D structure databases

ProteinModelPortaliO67605
SMRiO67605
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 24Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni125 – 127Substrate bindingUniRule annotation3
Regioni169 – 171Substrate bindingUniRule annotation3
Regioni185 – 187Allosteric activator ADP bindingUniRule annotation3
Regioni213 – 215Allosteric activator ADP bindingUniRule annotation3
Regioni252 – 255Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248870
InParanoidiO67605
KOiK00850
OMAiALYDLTF
OrthoDBiPOG091H01AC

Family and domain databases

HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

O67605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGILTAGG DAPGMNAVIR AVVRSAEREG HEVIGIKRGY KGLIEGDFIK
60 70 80 90 100
LTSRDVGGIL DRGGTILLSS RDPRYKKLEY REKAYKNLNE QGIEALIILG
110 120 130 140 150
GEGTLAGAKL TAEETGIPVI GVPCTIDNDV GGTEYCIGYD TALRNAVDAI
160 170 180 190 200
DKIRDTASSH ERIFIVEVMG RNRGFIAVEA GLATGAELIL IPEERFPREQ
210 220 230 240 250
IPEEIEKAKK LGKLHFIIVL AEGYCRAKEL EDFLLEKIGD KYGEIRSTVL
260 270 280 290 300
GHIQRGGIPT HFDRIMGTKF GVVAYESLIA GERSGFVAYE KGKFYIEDFE
310 320
KAQEYKPIDR EAYKLNTRLA I
Length:321
Mass (Da):35,409
Last modified:August 1, 1998 - v1
Checksum:i23C4602AAFAFF491
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC07573.1
PIRiC70447
RefSeqiNP_214171.1, NC_000918.1
WP_010881108.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC07573; AAC07573; aq_1708
GeneIDi1193320
KEGGiaae:aq_1708
PATRICifig|224324.8.peg.1312

Similar proteinsi

Entry informationi

Entry nameiPFKA_AQUAE
AccessioniPrimary (citable) accession number: O67605
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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