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O67605

- PFKA_AQUAE

UniProt

O67605 - PFKA_AQUAE

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, aq_1708
Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogen By similarity
Metal bindingi102 – 1021Magnesium; catalytic By similarity
Active sitei127 – 1271Proton acceptor By similarity
Binding sitei154 – 1541Allosteric activator ADP By similarity
Binding sitei162 – 1621Substrate; shared with dimeric partner By similarity
Binding sitei222 – 2221Substrate By similarity
Binding sitei246 – 2461Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 722ATP By similarity
Nucleotide bindingi101 – 1044ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1222-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:aq_1708
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_0000111931Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_1708.

Structurei

3D structure databases

ProteinModelPortaliO67605.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 245Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni125 – 1273Substrate binding By similarity
Regioni169 – 1713Substrate binding By similarity
Regioni185 – 1873Allosteric activator ADP binding By similarity
Regioni213 – 2153Allosteric activator ADP binding By similarity
Regioni252 – 2554Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiAKLERGH.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67605-1 [UniParc]FASTAAdd to Basket

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MRIGILTAGG DAPGMNAVIR AVVRSAEREG HEVIGIKRGY KGLIEGDFIK    50
LTSRDVGGIL DRGGTILLSS RDPRYKKLEY REKAYKNLNE QGIEALIILG 100
GEGTLAGAKL TAEETGIPVI GVPCTIDNDV GGTEYCIGYD TALRNAVDAI 150
DKIRDTASSH ERIFIVEVMG RNRGFIAVEA GLATGAELIL IPEERFPREQ 200
IPEEIEKAKK LGKLHFIIVL AEGYCRAKEL EDFLLEKIGD KYGEIRSTVL 250
GHIQRGGIPT HFDRIMGTKF GVVAYESLIA GERSGFVAYE KGKFYIEDFE 300
KAQEYKPIDR EAYKLNTRLA I 321
Length:321
Mass (Da):35,409
Last modified:August 1, 1998 - v1
Checksum:i23C4602AAFAFF491
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07573.1.
PIRiC70447.
RefSeqiNP_214171.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07573; AAC07573; aq_1708.
GeneIDi1193320.
KEGGiaae:aq_1708.
PATRICi20960414. VBIAquAeo85532_1312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07573.1 .
PIRi C70447.
RefSeqi NP_214171.1. NC_000918.1.

3D structure databases

ProteinModelPortali O67605.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_1708.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07573 ; AAC07573 ; aq_1708 .
GeneIDi 1193320.
KEGGi aae:aq_1708.
PATRICi 20960414. VBIAquAeo85532_1312.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248870.
KOi K00850.
OMAi AKLERGH.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci AAEO224324:GJBH-1222-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiPFKA_AQUAE
AccessioniPrimary (citable) accession number: O67605
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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