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O67605

- PFKA_AQUAE

UniProt

O67605 - PFKA_AQUAE

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATP; via amide nitrogenUniRule annotation
Metal bindingi102 – 1021Magnesium; catalyticUniRule annotation
Active sitei127 – 1271Proton acceptorUniRule annotation
Binding sitei154 – 1541Allosteric activator ADPUniRule annotation
Binding sitei162 – 1621Substrate; shared with dimeric partnerUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Binding sitei246 – 2461Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 722ATPUniRule annotation
Nucleotide bindingi101 – 1044ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1222-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:aq_1708
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321ATP-dependent 6-phosphofructokinasePRO_0000111931Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_1708.

Structurei

3D structure databases

ProteinModelPortaliO67605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 245Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni125 – 1273Substrate bindingUniRule annotation
Regioni169 – 1713Substrate bindingUniRule annotation
Regioni185 – 1873Allosteric activator ADP bindingUniRule annotation
Regioni213 – 2153Allosteric activator ADP bindingUniRule annotation
Regioni252 – 2554Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
InParanoidiO67605.
KOiK00850.
OMAiAKLERGH.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67605-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIGILTAGG DAPGMNAVIR AVVRSAEREG HEVIGIKRGY KGLIEGDFIK
60 70 80 90 100
LTSRDVGGIL DRGGTILLSS RDPRYKKLEY REKAYKNLNE QGIEALIILG
110 120 130 140 150
GEGTLAGAKL TAEETGIPVI GVPCTIDNDV GGTEYCIGYD TALRNAVDAI
160 170 180 190 200
DKIRDTASSH ERIFIVEVMG RNRGFIAVEA GLATGAELIL IPEERFPREQ
210 220 230 240 250
IPEEIEKAKK LGKLHFIIVL AEGYCRAKEL EDFLLEKIGD KYGEIRSTVL
260 270 280 290 300
GHIQRGGIPT HFDRIMGTKF GVVAYESLIA GERSGFVAYE KGKFYIEDFE
310 320
KAQEYKPIDR EAYKLNTRLA I
Length:321
Mass (Da):35,409
Last modified:August 1, 1998 - v1
Checksum:i23C4602AAFAFF491
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07573.1.
PIRiC70447.
RefSeqiNP_214171.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07573; AAC07573; aq_1708.
GeneIDi1193320.
KEGGiaae:aq_1708.
PATRICi20960414. VBIAquAeo85532_1312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07573.1 .
PIRi C70447.
RefSeqi NP_214171.1. NC_000918.1.

3D structure databases

ProteinModelPortali O67605.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_1708.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07573 ; AAC07573 ; aq_1708 .
GeneIDi 1193320.
KEGGi aae:aq_1708.
PATRICi 20960414. VBIAquAeo85532_1312.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248870.
InParanoidi O67605.
KOi K00850.
OMAi AKLERGH.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci AAEO224324:GJBH-1222-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiPFKA_AQUAE
AccessioniPrimary (citable) accession number: O67605
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3