Reviewed, UniProtKB/Swiss-Prot O67604 (RISA_AQUAE)

Names and origin

Protein names

Recommended name:
    Riboflavin synthase alpha chain
    EC=2.5.1.9

Gene names

Name:	ribE
Synonyms:	ribC
Ordered Locus Names:	aq_1707

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil and riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine: step 1/1.
Subunit structure	Oligomer that consist of 3 alpha subunits and 60 beta subunits By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Riboflavin synthase alpha chain		PRO_0000068157
Regions
	Repeat	1 – 94	94	Lumazine-binding 1
	Repeat	95 – 191	97	Lumazine-binding 2

Sequences

	Sequence		Length	Mass (Da)
	O67604-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 322A09FF7B15E432	FASTA	207	23,196
	10 20 30 40 50 60 MFTGLVEDLG KVKNLTLSSK GAKLSVETKL EDVKLGDSVS VNGACLTVVD IKSSTLTFDV 70 80 90 100 110 120 SPETLKRTNL GKLKTGDYVN LERALRVGER LGGHIVQGHV DFTAPVKSFN FLGEHYELVI 130 140 150 160 170 180 EIPEEWSIYV VEKGSIALDG ISLTVNYVKE NKVFINIIPH TYKSTNLQFK KVGDLLNVET 190 200 DILGKYVINY LNKLKKKEDI FKEFLKW

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07572.1.
PIR	B70447.
RefSeq	NP_214170.1.
3D structure databases
HSSP	HSSP built from PDB template 1KZL based on UniProtKB Q9Y7P0.
ModBase	Search...
Genome annotation databases
GeneID	1193319.
GenomeReviews	Gene locus aq_1707 in contig AE000657_GR.
KEGG	aae:aq_1707.
NMPDR	fig|224324.1.peg.1185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67604.
OMA	GDRLGGH.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1707-MON.
BRENDA	2.5.1.9. 189781.
Family and domain databases
InterPro	IPR001783. Lumazine_bd. [Graphical view]
PANTHER	PTHR21098. Lumazine_bd. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
ProDom	PD004110. Lum_binding. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00187. ribE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_AQUAE

Accession

Primary (citable) accession number: O67604

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)