O67589 (SYD_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	aq_1677

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 603	603	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_0000110818

Sequences

	Sequence		Length	Mass (Da)
	O67589 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 9DFFBD840C8DCC1C	FASTA	603	69,729
	10 20 30 40 50 60 MLTEYGDFKR TKYCGEVSEE DIGKEVKLAG WVHRKRHHGG VIFIDLRDRE GIVQVVVEEK 70 80 90 100 110 120 TNPEAYEVAD KLKSEYVIGV VGKVRKRPEG TENPKLKTGY VEVVADRILV FNTSEALPFP 130 140 150 160 170 180 VEEETHVSEE TKLKYRYIDL RRESMKNNLI FRHRVYQITR NFFTKEGFIE IETPFLTKST 190 200 210 220 230 240 PEGARDFLVP SRLHPGKFYA LPQSPQLFKQ ILMIAGFDRY FQIVKCFRDE DLRADRQPEF 250 260 270 280 290 300 TQIDYEMSFV SEEEVMDVAE RLIATLFKEL LGVELKTPFE RISYREAMEK YGTDKPDRRF 310 320 330 340 350 360 GLELIELTDI FKNTAFKVFK SVVEAGGIIK AINFKGSNLS RKEIDELTKF VQSLGAKGLA 370 380 390 400 410 420 WIKVEKDKLT SPIVKFFTEE ETQKLLERTK AEPGDVILFS ADKKEMVYKI LGNLRLHLGK 430 440 450 460 470 480 KYKLIDESKW DVFWIVDFPL MEWDEEEERF VSLHHPFTMP REENIPKLKE ALEEEDLEKK 490 500 510 520 530 540 KEIVHSVRAR AYDMVLNGEE IGGGSIRIHR RDIQEVVFKL LGIGEVEAQE KFGFLLEALK 550 560 570 580 590 600 YGAPPHGGLA FGLDRVVALM LGLDSIRDTI AFPKTQRGIC PLTGAPDYVD PKQLKELHIK VLE

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07548.1.
PIR	D70445.
RefSeq	NP_214155.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67589.
SMR	O67589. Positions 8-602.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193305.
GenomeReviews	Gene locus aq_1677 in contig AE000657_GR.
KEGG	aae:aq_1677.
NMPDR	fig|224324.1.peg.1170.
PATRIC	20960370. VBIAquAeo85532_1296.
Phylogenomic databases
HOGENOM	HBG396032.
OMA	AFPKTQQ.
PhylomeDB	O67589.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1677-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_AQUAE

Accession

Primary (citable) accession number: O67589

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program