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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi340 – 347ATP 1By similarity8
Nucleotide bindingi726 – 733ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:aq_1672
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001910871 – 1006Chaperone protein ClpBAdd BLAST1006

Proteomic databases

PRIDEiO67588.

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

STRINGi224324.aq_1672.

Structurei

3D structure databases

ProteinModelPortaliO67588.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini603 – 638UVRPROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 232N-terminalBy similarityAdd BLAST232
Regioni251 – 473NBD1By similarityAdd BLAST223
Regioni474 – 666LinkerBy similarityAdd BLAST193
Regioni676 – 893NBD2By similarityAdd BLAST218
Regioni894 – 1006C-terminalBy similarityAdd BLAST113

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili524 – 648By similarityAdd BLAST125

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated
Contains 1 UVR domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218211.
InParanoidiO67588.
KOiK03695.
OMAiHESIIGQ.
OrthoDBiPOG091H019M.

Family and domain databases

Gene3Di1.10.1780.10. 2 hits.
3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67588-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISENLFSAK SLEYLEKAKE IARENKDTKV DTDHLLIALL LDEKSPLSKY
60 70 80 90 100
IEKRGIDRKE FLKRVSEYLE NLNKQLEKAI EAEAQNLINL RTQIMEVKSN
110 120 130 140 150
IGNVQTELSK IRKAKERILR ELEYARRYGD WWTAETLELE LRQLEATEQS
160 170 180 190 200
IRRQLEQVEK SLSTVFAPED VKAFLENKLS IDGLIRKALE KSPLIEQVKE
210 220 230 240 250
LGISPDRIID KVAEKVFGKK PTFDYSQYLT QVLEKAQDKA VSEGEAQVQP
260 270 280 290 300
SHISAALIEA KDTIGGKLIN QVLGGEKEMK KDVTQELKEE EKSPLERFGV
310 320 330 340 350
NLNELARQGK LDPVIGRERE INQVIEILLR RTKNNPVLVG DPGVGKTAIV
360 370 380 390 400
EGLAQRIVNK EVPPELQDKE IWSIDMASLL AGSKYRGEFE ERLKALLDEV
410 420 430 440 450
KEKGNVILFI DEIHTVVGAG AAEGAVDAAN IMKPALARGE IRVIGATTVD
460 470 480 490 500
EYRKYIEKDP ALERRFQPVF VDEPTEEQTI EILKGLRPRL EQFHKVKISD
510 520 530 540 550
EAIEAAVKLT KRYVTFRRLP DKAIDALDQA AARKKLKVIG TPPEIQEIER
560 570 580 590 600
KIKALEEQII EANLKGDYEK EAQLKIEKAK LEKEKQELLG KVGGVEAKIA
610 620 630 640 650
ELKKKIEELD EKIKEAAEKG DYEKEAELKI EKAKLEKELK KLEQEKSKEL
660 670 680 690 700
VVTWDDVAEV VSEWTGIPVS RLKEEEMQKL LKLEDELHKR VVDQEHAVKA
710 720 730 740 750
VAEAIRRARA GLKDPKRPIA SFLFLGPTGV GKTELSKALA ELLFGDEDAL
760 770 780 790 800
IRLDMSEFKE EHSVAKLIGA PPGYVGYEEG GKLTEAVRRK PYSVILLDEI
810 820 830 840 850
EKAHPRVLDL FLQVLDDGRL TDSHGRTVDF RNTVIIMTSN IGSQYLLNIP
860 870 880 890 900
VDADEETLNR EFEKAKEKVL EELKLYMRPE FINRIDEIIV FKPLTMRELS
910 920 930 940 950
KIIDLLIANV NKRLAERNIK IELTEEAKKE LVRRGYDPAF GARPLKRTIQ
960 970 980 990 1000
KYVETPLADK IIRGEIKDGM TVVVDYKDGE FVFIPKEEYE KQKAEVSASS

EEKKES
Length:1,006
Mass (Da):114,537
Last modified:August 1, 1998 - v1
Checksum:iFEBB850E75D1A8EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07550.1.
PIRiC70445.
RefSeqiNP_214154.1. NC_000918.1.
WP_010881091.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07550; AAC07550; aq_1672.
GeneIDi1193304.
KEGGiaae:aq_1672.
PATRICi20960368. VBIAquAeo85532_1295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07550.1.
PIRiC70445.
RefSeqiNP_214154.1. NC_000918.1.
WP_010881091.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67588.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1672.

Proteomic databases

PRIDEiO67588.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07550; AAC07550; aq_1672.
GeneIDi1193304.
KEGGiaae:aq_1672.
PATRICi20960368. VBIAquAeo85532_1295.

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218211.
InParanoidiO67588.
KOiK03695.
OMAiHESIIGQ.
OrthoDBiPOG091H019M.

Family and domain databases

Gene3Di1.10.1780.10. 2 hits.
3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPB_AQUAE
AccessioniPrimary (citable) accession number: O67588
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.