Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O67588

- CLPB_AQUAE

UniProt

O67588 - CLPB_AQUAE

Protein

Chaperone protein ClpB

Gene

clpB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi340 – 3478ATP 1By similarity
    Nucleotide bindingi726 – 7338ATP 2By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. nucleoside-triphosphatase activity Source: InterPro

    GO - Biological processi

    1. protein metabolic process Source: InterPro
    2. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1199-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chaperone protein ClpB
    Gene namesi
    Name:clpB
    Ordered Locus Names:aq_1672
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10061006Chaperone protein ClpBPRO_0000191087Add
    BLAST

    Proteomic databases

    PRIDEiO67588.

    Interactioni

    Subunit structurei

    Homohexamer. The oligomerization is ATP-dependent By similarity.By similarity

    Protein-protein interaction databases

    STRINGi224324.aq_1672.

    Structurei

    3D structure databases

    ProteinModelPortaliO67588.
    SMRiO67588. Positions 295-484.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini603 – 63836UVRPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 232232N-terminalBy similarityAdd
    BLAST
    Regioni251 – 473223NBD1By similarityAdd
    BLAST
    Regioni474 – 666193LinkerBy similarityAdd
    BLAST
    Regioni676 – 893218NBD2By similarityAdd
    BLAST
    Regioni894 – 1006113C-terminalBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili524 – 648125By similarityAdd
    BLAST

    Domaini

    The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer By similarity.By similarity

    Sequence similaritiesi

    Belongs to the ClpA/ClpB family.Curated
    Contains 1 UVR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiCOG0542.
    HOGENOMiHOG000218211.
    KOiK03695.
    OMAiKNAFRPE.
    OrthoDBiEOG65F8SM.

    Family and domain databases

    Gene3Di1.10.1780.10. 2 hits.
    3.40.50.300. 3 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR019489. Clp_ATPase_C.
    IPR004176. Clp_N.
    IPR001270. ClpA/B.
    IPR018368. ClpA/B_CS1.
    IPR028299. ClpA/B_CS2.
    IPR023150. Dbl_Clp-N.
    IPR027417. P-loop_NTPase.
    IPR001943. UVR_dom.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    PF07724. AAA_2. 1 hit.
    PF02861. Clp_N. 1 hit.
    PF10431. ClpB_D2-small. 1 hit.
    [Graphical view]
    PRINTSiPR00300. CLPPROTEASEA.
    SMARTiSM00382. AAA. 2 hits.
    SM01086. ClpB_D2-small. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00870. CLPAB_1. 1 hit.
    PS00871. CLPAB_2. 1 hit.
    PS50151. UVR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O67588-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISENLFSAK SLEYLEKAKE IARENKDTKV DTDHLLIALL LDEKSPLSKY     50
    IEKRGIDRKE FLKRVSEYLE NLNKQLEKAI EAEAQNLINL RTQIMEVKSN 100
    IGNVQTELSK IRKAKERILR ELEYARRYGD WWTAETLELE LRQLEATEQS 150
    IRRQLEQVEK SLSTVFAPED VKAFLENKLS IDGLIRKALE KSPLIEQVKE 200
    LGISPDRIID KVAEKVFGKK PTFDYSQYLT QVLEKAQDKA VSEGEAQVQP 250
    SHISAALIEA KDTIGGKLIN QVLGGEKEMK KDVTQELKEE EKSPLERFGV 300
    NLNELARQGK LDPVIGRERE INQVIEILLR RTKNNPVLVG DPGVGKTAIV 350
    EGLAQRIVNK EVPPELQDKE IWSIDMASLL AGSKYRGEFE ERLKALLDEV 400
    KEKGNVILFI DEIHTVVGAG AAEGAVDAAN IMKPALARGE IRVIGATTVD 450
    EYRKYIEKDP ALERRFQPVF VDEPTEEQTI EILKGLRPRL EQFHKVKISD 500
    EAIEAAVKLT KRYVTFRRLP DKAIDALDQA AARKKLKVIG TPPEIQEIER 550
    KIKALEEQII EANLKGDYEK EAQLKIEKAK LEKEKQELLG KVGGVEAKIA 600
    ELKKKIEELD EKIKEAAEKG DYEKEAELKI EKAKLEKELK KLEQEKSKEL 650
    VVTWDDVAEV VSEWTGIPVS RLKEEEMQKL LKLEDELHKR VVDQEHAVKA 700
    VAEAIRRARA GLKDPKRPIA SFLFLGPTGV GKTELSKALA ELLFGDEDAL 750
    IRLDMSEFKE EHSVAKLIGA PPGYVGYEEG GKLTEAVRRK PYSVILLDEI 800
    EKAHPRVLDL FLQVLDDGRL TDSHGRTVDF RNTVIIMTSN IGSQYLLNIP 850
    VDADEETLNR EFEKAKEKVL EELKLYMRPE FINRIDEIIV FKPLTMRELS 900
    KIIDLLIANV NKRLAERNIK IELTEEAKKE LVRRGYDPAF GARPLKRTIQ 950
    KYVETPLADK IIRGEIKDGM TVVVDYKDGE FVFIPKEEYE KQKAEVSASS 1000
    EEKKES 1006
    Length:1,006
    Mass (Da):114,537
    Last modified:August 1, 1998 - v1
    Checksum:iFEBB850E75D1A8EF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07550.1.
    PIRiC70445.
    RefSeqiNP_214154.1. NC_000918.1.
    WP_010881091.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07550; AAC07550; aq_1672.
    GeneIDi1193304.
    KEGGiaae:aq_1672.
    PATRICi20960368. VBIAquAeo85532_1295.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07550.1 .
    PIRi C70445.
    RefSeqi NP_214154.1. NC_000918.1.
    WP_010881091.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O67588.
    SMRi O67588. Positions 295-484.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_1672.

    Proteomic databases

    PRIDEi O67588.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07550 ; AAC07550 ; aq_1672 .
    GeneIDi 1193304.
    KEGGi aae:aq_1672.
    PATRICi 20960368. VBIAquAeo85532_1295.

    Phylogenomic databases

    eggNOGi COG0542.
    HOGENOMi HOG000218211.
    KOi K03695.
    OMAi KNAFRPE.
    OrthoDBi EOG65F8SM.

    Enzyme and pathway databases

    BioCyci AAEO224324:GJBH-1199-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1780.10. 2 hits.
    3.40.50.300. 3 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR019489. Clp_ATPase_C.
    IPR004176. Clp_N.
    IPR001270. ClpA/B.
    IPR018368. ClpA/B_CS1.
    IPR028299. ClpA/B_CS2.
    IPR023150. Dbl_Clp-N.
    IPR027417. P-loop_NTPase.
    IPR001943. UVR_dom.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    PF07724. AAA_2. 1 hit.
    PF02861. Clp_N. 1 hit.
    PF10431. ClpB_D2-small. 1 hit.
    [Graphical view ]
    PRINTSi PR00300. CLPPROTEASEA.
    SMARTi SM00382. AAA. 2 hits.
    SM01086. ClpB_D2-small. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00870. CLPAB_1. 1 hit.
    PS00871. CLPAB_2. 1 hit.
    PS50151. UVR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiCLPB_AQUAE
    AccessioniPrimary (citable) accession number: O67588
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3