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O67588

- CLPB_AQUAE

UniProt

O67588 - CLPB_AQUAE

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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi340 – 3478ATP 1By similarity
Nucleotide bindingi726 – 7338ATP 2By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. protein metabolic process Source: InterPro
  2. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1199-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:aq_1672
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10061006Chaperone protein ClpBPRO_0000191087Add
BLAST

Proteomic databases

PRIDEiO67588.

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

STRINGi224324.aq_1672.

Structurei

3D structure databases

ProteinModelPortaliO67588.
SMRiO67588. Positions 295-484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini603 – 63836UVRPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 232232N-terminalBy similarityAdd
BLAST
Regioni251 – 473223NBD1By similarityAdd
BLAST
Regioni474 – 666193LinkerBy similarityAdd
BLAST
Regioni676 – 893218NBD2By similarityAdd
BLAST
Regioni894 – 1006113C-terminalBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili524 – 648125By similarityAdd
BLAST

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated
Contains 1 UVR domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG0542.
HOGENOMiHOG000218211.
InParanoidiO67588.
KOiK03695.
OMAiKNAFRPE.
OrthoDBiEOG65F8SM.

Family and domain databases

Gene3Di1.10.1780.10. 2 hits.
3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR023150. Dbl_Clp-N.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67588-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MISENLFSAK SLEYLEKAKE IARENKDTKV DTDHLLIALL LDEKSPLSKY
60 70 80 90 100
IEKRGIDRKE FLKRVSEYLE NLNKQLEKAI EAEAQNLINL RTQIMEVKSN
110 120 130 140 150
IGNVQTELSK IRKAKERILR ELEYARRYGD WWTAETLELE LRQLEATEQS
160 170 180 190 200
IRRQLEQVEK SLSTVFAPED VKAFLENKLS IDGLIRKALE KSPLIEQVKE
210 220 230 240 250
LGISPDRIID KVAEKVFGKK PTFDYSQYLT QVLEKAQDKA VSEGEAQVQP
260 270 280 290 300
SHISAALIEA KDTIGGKLIN QVLGGEKEMK KDVTQELKEE EKSPLERFGV
310 320 330 340 350
NLNELARQGK LDPVIGRERE INQVIEILLR RTKNNPVLVG DPGVGKTAIV
360 370 380 390 400
EGLAQRIVNK EVPPELQDKE IWSIDMASLL AGSKYRGEFE ERLKALLDEV
410 420 430 440 450
KEKGNVILFI DEIHTVVGAG AAEGAVDAAN IMKPALARGE IRVIGATTVD
460 470 480 490 500
EYRKYIEKDP ALERRFQPVF VDEPTEEQTI EILKGLRPRL EQFHKVKISD
510 520 530 540 550
EAIEAAVKLT KRYVTFRRLP DKAIDALDQA AARKKLKVIG TPPEIQEIER
560 570 580 590 600
KIKALEEQII EANLKGDYEK EAQLKIEKAK LEKEKQELLG KVGGVEAKIA
610 620 630 640 650
ELKKKIEELD EKIKEAAEKG DYEKEAELKI EKAKLEKELK KLEQEKSKEL
660 670 680 690 700
VVTWDDVAEV VSEWTGIPVS RLKEEEMQKL LKLEDELHKR VVDQEHAVKA
710 720 730 740 750
VAEAIRRARA GLKDPKRPIA SFLFLGPTGV GKTELSKALA ELLFGDEDAL
760 770 780 790 800
IRLDMSEFKE EHSVAKLIGA PPGYVGYEEG GKLTEAVRRK PYSVILLDEI
810 820 830 840 850
EKAHPRVLDL FLQVLDDGRL TDSHGRTVDF RNTVIIMTSN IGSQYLLNIP
860 870 880 890 900
VDADEETLNR EFEKAKEKVL EELKLYMRPE FINRIDEIIV FKPLTMRELS
910 920 930 940 950
KIIDLLIANV NKRLAERNIK IELTEEAKKE LVRRGYDPAF GARPLKRTIQ
960 970 980 990 1000
KYVETPLADK IIRGEIKDGM TVVVDYKDGE FVFIPKEEYE KQKAEVSASS

EEKKES
Length:1,006
Mass (Da):114,537
Last modified:August 1, 1998 - v1
Checksum:iFEBB850E75D1A8EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07550.1.
PIRiC70445.
RefSeqiNP_214154.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07550; AAC07550; aq_1672.
GeneIDi1193304.
KEGGiaae:aq_1672.
PATRICi20960368. VBIAquAeo85532_1295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07550.1 .
PIRi C70445.
RefSeqi NP_214154.1. NC_000918.1.

3D structure databases

ProteinModelPortali O67588.
SMRi O67588. Positions 295-484.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_1672.

Proteomic databases

PRIDEi O67588.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07550 ; AAC07550 ; aq_1672 .
GeneIDi 1193304.
KEGGi aae:aq_1672.
PATRICi 20960368. VBIAquAeo85532_1295.

Phylogenomic databases

eggNOGi COG0542.
HOGENOMi HOG000218211.
InParanoidi O67588.
KOi K03695.
OMAi KNAFRPE.
OrthoDBi EOG65F8SM.

Enzyme and pathway databases

BioCyci AAEO224324:GJBH-1199-MONOMER.

Family and domain databases

Gene3Di 1.10.1780.10. 2 hits.
3.40.50.300. 3 hits.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR023150. Dbl_Clp-N.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view ]
PRINTSi PR00300. CLPPROTEASEA.
SMARTi SM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiCLPB_AQUAE
AccessioniPrimary (citable) accession number: O67588
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3