O67588 (CLPB_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chaperone protein ClpB

Gene names

Name:	clpB
Ordered Locus Names:	aq_1672

Organism

Aquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Aquificae › Aquificae › Aquificales › Aquificaceae › Aquifex › Aquifex aeolicus

Protein attributes

Sequence length	1006 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK By similarity.
Subunit structure	Homohexamer. The oligomerization is ATP-dependent By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer By similarity.
Sequence similarities	Belongs to the clpA/clpB family. Contains 1 UVR domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Domain	Coiled coil Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein metabolic process Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1006	1006	Chaperone protein ClpB		PRO_0000191087
Regions
	Domain	603 – 638	36	UVR
	Nucleotide binding	340 – 347	8	ATP 1 By similarity
	Nucleotide binding	726 – 733	8	ATP 2 By similarity
	Region	1 – 232	232	N-terminal By similarity
	Region	251 – 473	223	NBD1 By similarity
	Region	474 – 666	193	Linker By similarity
	Region	676 – 893	218	NBD2 By similarity
	Region	894 – 1006	113	C-terminal By similarity
	Coiled coil	524 – 648	125	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67588 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: FEBB850E75D1A8EF	FASTA	1,006	114,537
	10 20 30 40 50 60 MISENLFSAK SLEYLEKAKE IARENKDTKV DTDHLLIALL LDEKSPLSKY IEKRGIDRKE 70 80 90 100 110 120 FLKRVSEYLE NLNKQLEKAI EAEAQNLINL RTQIMEVKSN IGNVQTELSK IRKAKERILR 130 140 150 160 170 180 ELEYARRYGD WWTAETLELE LRQLEATEQS IRRQLEQVEK SLSTVFAPED VKAFLENKLS 190 200 210 220 230 240 IDGLIRKALE KSPLIEQVKE LGISPDRIID KVAEKVFGKK PTFDYSQYLT QVLEKAQDKA 250 260 270 280 290 300 VSEGEAQVQP SHISAALIEA KDTIGGKLIN QVLGGEKEMK KDVTQELKEE EKSPLERFGV 310 320 330 340 350 360 NLNELARQGK LDPVIGRERE INQVIEILLR RTKNNPVLVG DPGVGKTAIV EGLAQRIVNK 370 380 390 400 410 420 EVPPELQDKE IWSIDMASLL AGSKYRGEFE ERLKALLDEV KEKGNVILFI DEIHTVVGAG 430 440 450 460 470 480 AAEGAVDAAN IMKPALARGE IRVIGATTVD EYRKYIEKDP ALERRFQPVF VDEPTEEQTI 490 500 510 520 530 540 EILKGLRPRL EQFHKVKISD EAIEAAVKLT KRYVTFRRLP DKAIDALDQA AARKKLKVIG 550 560 570 580 590 600 TPPEIQEIER KIKALEEQII EANLKGDYEK EAQLKIEKAK LEKEKQELLG KVGGVEAKIA 610 620 630 640 650 660 ELKKKIEELD EKIKEAAEKG DYEKEAELKI EKAKLEKELK KLEQEKSKEL VVTWDDVAEV 670 680 690 700 710 720 VSEWTGIPVS RLKEEEMQKL LKLEDELHKR VVDQEHAVKA VAEAIRRARA GLKDPKRPIA 730 740 750 760 770 780 SFLFLGPTGV GKTELSKALA ELLFGDEDAL IRLDMSEFKE EHSVAKLIGA PPGYVGYEEG 790 800 810 820 830 840 GKLTEAVRRK PYSVILLDEI EKAHPRVLDL FLQVLDDGRL TDSHGRTVDF RNTVIIMTSN 850 860 870 880 890 900 IGSQYLLNIP VDADEETLNR EFEKAKEKVL EELKLYMRPE FINRIDEIIV FKPLTMRELS 910 920 930 940 950 960 KIIDLLIANV NKRLAERNIK IELTEEAKKE LVRRGYDPAF GARPLKRTIQ KYVETPLADK 970 980 990 1000 IIRGEIKDGM TVVVDYKDGE FVFIPKEEYE KQKAEVSASS EEKKES

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07550.1.
PIR	C70445.
RefSeq	NP_214154.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67588.
SMR	O67588. Positions 295-484.
ModBase	Search...
Protein-protein interaction databases
STRING	224324.aq_1672.
Proteomic databases
PRIDE	O67588.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC07550; AAC07550; aq_1672.
GeneID	1193304.
KEGG	aae:aq_1672.
PATRIC	20960368. VBIAquAeo85532_1295.
Phylogenomic databases
eggNOG	COG0542.
HOGENOM	HOG000218211.
KO	K03695.
OMA	WSIDMAS.
ProtClustDB	CLSK2299794.
Enzyme and pathway databases
BioCyc	AAEO224324:GJBH-1199-MONOMER.
Family and domain databases
Gene3D	1.10.1780.10. 2 hits.
InterPro	IPR003593. AAA+_ATPase. IPR013093. ATPase_AAA-2. IPR003959. ATPase_AAA_core. IPR018368. Chaperonin_ClpA/B_CS. IPR001270. Chaprnin_ClpA/B. IPR019489. Clp_ATPase_C. IPR004176. Clp_N. IPR023150. Dbl_Clp-N. IPR001943. UVR_dom. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF07724. AAA_2. 1 hit. PF02861. Clp_N. 1 hit. PF10431. ClpB_D2-small. 1 hit. [Graphical view]
PRINTS	PR00300. CLPPROTEASEA.
SMART	SM00382. AAA. 2 hits. SM01086. ClpB_D2-small. 1 hit. [Graphical view]
PROSITE	PS00870. CLPAB_1. 1 hit. PS00871. CLPAB_2. 1 hit. PS50151. UVR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPB_AQUAE

Accession

Primary (citable) accession number: O67588

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program