O67583 (SYT_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	aq_1667

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 638	638	Threonine--tRNA ligase HAMAP MF_00184		PRO_0000100933
Regions
	Region	243 – 536	294	Catalytic HAMAP MF_00184
Sites
	Metal binding	336	1	Zinc; catalytic By similarity
	Metal binding	387	1	Zinc; catalytic By similarity
	Metal binding	513	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67583 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5ECBA8227C047955	FASTA	638	74,097
	10 20 30 40 50 60 MEKIKVKIKG KEYEVEKGTP LGKIFELAGI KDALGGVING KIIDLQTPVR ESGEIKPVYR 70 80 90 100 110 120 GSKESLEIMR HSLAHIMAQA LKELYGAKKV HLGVGPTTEE GFYYDVEVEG HKITEEDLPK 130 140 150 160 170 180 IEQKMKEIIE RDYPILRREL SREEAIKLFD KLKEKYKIDI IKEIPEEEVI SVYEQGDFID 190 200 210 220 230 240 LCKGPHLPST GKAGAFKLTS ISGAYWKGRS DQPQLTRIYG IAYWSDKEVK ERLKFYEEVK 250 260 270 280 290 300 KRDHRRLGKE LEFFTIDDNV GAGLILWLPR GAIYRKVLED YLREEHLKRG YQLVYTPHVG 310 320 330 340 350 360 KSKLWETSGH LECYKQNMFP SMKIDEEEYY VKPMNCPFHI AIYKSRTRSY KELPLKLFEL 370 380 390 400 410 420 GTVYRYELSG VLHGLLRVRG FTQDDAHIVC TPEQVNDVIR ETLDFALSTL KDFGFNEFKI 430 440 450 460 470 480 YLSTRPEYSI GSDEQWEVSQ NALKKAIEDL GYEYEIDEGG GAFYGPKIDV KIRDAIGRMW 490 500 510 520 530 540 QLSTIQFDFN LPERFDMTYV GPDNKKHRPY MIHRALLGSI ERFTGILLEH YAGLLPIWLS 550 560 570 580 590 600 PTQVMIIPIA DRHHEYAKKV YEFLKENGIR AEMDLREERM NAKIRDAELK KIPVILVVGD 610 620 630 REAQNNTVSV RTKKEGNLGS MELNKFLDWI KEKIKNKE

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07549.1.
PIR	F70444.
RefSeq	NP_214149.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67583.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193299.
GenomeReviews	Gene locus aq_1667 in contig AE000657_GR.
KEGG	aae:aq_1667.
NMPDR	fig|224324.1.peg.1164.
PATRIC	20960356. VBIAquAeo85532_1290.
Phylogenomic databases
HOGENOM	HBG352811.
OMA	RTAMEDY.
PhylomeDB	O67583.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1667-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_AQUAE

Accession

Primary (citable) accession number: O67583

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program