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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).UniRule annotation

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi336ZincUniRule annotation1
Metal bindingi387Zinc; via tele nitrogenUniRule annotation1
Metal bindingi513Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-1187-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:aq_1667
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001009331 – 638Threonine--tRNA ligaseAdd BLAST638

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_1667

Structurei

3D structure databases

ProteinModelPortaliO67583
SMRiO67583
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni243 – 536CatalyticUniRule annotationAdd BLAST294

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C22 Bacteria
COG0441 LUCA
HOGENOMiHOG000003880
InParanoidiO67583
KOiK01868
OMAiFYYDFAY
OrthoDBiPOG091H01SS

Family and domain databases

CDDicd00771 ThrRS_core, 1 hit
Gene3Di3.40.50.8001 hit
HAMAPiMF_00184 Thr_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR002320 Thr-tRNA-ligase_IIa
IPR018163 Thr/Ala-tRNA-synth_IIc_edit
IPR033728 ThrRS_core
IPR012947 tRNA_SAD
PANTHERiPTHR11451 PTHR11451, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF07973 tRNA_SAD, 1 hit
PRINTSiPR01047 TRNASYNTHTHR
SMARTiView protein in SMART
SM00863 tRNA_SAD, 1 hit
SUPFAMiSSF55186 SSF55186, 1 hit
TIGRFAMsiTIGR00418 thrS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

O67583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKIKVKIKG KEYEVEKGTP LGKIFELAGI KDALGGVING KIIDLQTPVR
60 70 80 90 100
ESGEIKPVYR GSKESLEIMR HSLAHIMAQA LKELYGAKKV HLGVGPTTEE
110 120 130 140 150
GFYYDVEVEG HKITEEDLPK IEQKMKEIIE RDYPILRREL SREEAIKLFD
160 170 180 190 200
KLKEKYKIDI IKEIPEEEVI SVYEQGDFID LCKGPHLPST GKAGAFKLTS
210 220 230 240 250
ISGAYWKGRS DQPQLTRIYG IAYWSDKEVK ERLKFYEEVK KRDHRRLGKE
260 270 280 290 300
LEFFTIDDNV GAGLILWLPR GAIYRKVLED YLREEHLKRG YQLVYTPHVG
310 320 330 340 350
KSKLWETSGH LECYKQNMFP SMKIDEEEYY VKPMNCPFHI AIYKSRTRSY
360 370 380 390 400
KELPLKLFEL GTVYRYELSG VLHGLLRVRG FTQDDAHIVC TPEQVNDVIR
410 420 430 440 450
ETLDFALSTL KDFGFNEFKI YLSTRPEYSI GSDEQWEVSQ NALKKAIEDL
460 470 480 490 500
GYEYEIDEGG GAFYGPKIDV KIRDAIGRMW QLSTIQFDFN LPERFDMTYV
510 520 530 540 550
GPDNKKHRPY MIHRALLGSI ERFTGILLEH YAGLLPIWLS PTQVMIIPIA
560 570 580 590 600
DRHHEYAKKV YEFLKENGIR AEMDLREERM NAKIRDAELK KIPVILVVGD
610 620 630
REAQNNTVSV RTKKEGNLGS MELNKFLDWI KEKIKNKE
Length:638
Mass (Da):74,097
Last modified:August 1, 1998 - v1
Checksum:i5ECBA8227C047955
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC07549.1
PIRiF70444
RefSeqiNP_214149.1, NC_000918.1
WP_010881086.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC07549; AAC07549; aq_1667
GeneIDi1193299
KEGGiaae:aq_1667
PATRICifig|224324.8.peg.1290

Similar proteinsi

Entry informationi

Entry nameiSYT_AQUAE
AccessioniPrimary (citable) accession number: O67583
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: February 28, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome