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O67581 (MDH2_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase 2

EC=1.1.1.37
Gene names
Name:mdh2
Ordered Locus Names:aq_1665
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Malate dehydrogenase 2 HAMAP-Rule MF_00487
PRO_0000113423

Regions

Nucleotide binding20 – 256NAD By similarity
Nucleotide binding136 – 1383NAD By similarity

Sites

Active site1931Proton acceptor By similarity
Binding site1001Substrate By similarity
Binding site1061Substrate By similarity
Binding site1131NAD By similarity
Binding site1381Substrate By similarity
Binding site1691Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O67581 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 18E64807D16B7C96

FASTA33436,698
        10         20         30         40         50         60 
MKGKLINSFL MKKPKISVIG AGKVGENVAY LLTILGLGDV YLFARYKKGL EPAKAKALDL 

        70         80         90        100        110        120 
KQMAVLMDID INVKGISYDK EGFEELKGSD IVVITAGIPR REGMSREDLL YENLKILKKF 

       130        140        150        160        170        180 
TDAIKEYAKD SIIIVVSNPV DTLTYATIKL TGFEPRRVIG MAGVLDSARF KNFVKEKIGI 

       190        200        210        220        230        240 
SNADIRTLVL GTHGDLMVPV TSHSFIGDKP IEEVFSASEI DELIEKTRKG GAQIVSLMGT 

       250        260        270        280        290        300 
SAYYAPAASV VIMVESIIND RKRVMPCSVY VEGEAAKHYE IEGVCIGLPV VLGKKGVEDF 

       310        320        330 
ELVNLSGYEK RELLRSAKTL KEMVSLADKL LNEL 

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC07547.1.
PIRD70444.
RefSeqNP_214147.1. NC_000918.1.

3D structure databases

ProteinModelPortalO67581.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_1665.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC07547; AAC07547; aq_1665.
GeneID1193297.
KEGGaae:aq_1665.
PATRIC20960352. VBIAquAeo85532_1288.

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213794.
KOK00024.
OMAYGVAMML.
OrthoDBEOG6091FG.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-1191-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH2_AQUAE
AccessionPrimary (citable) accession number: O67581
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families