Reviewed, UniProtKB/Swiss-Prot O67581 (MDH2_AQUAE)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase 2
    EC=1.1.1.37

Gene names

Name:	mdh2
Ordered Locus Names:	aq_1665

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Malate dehydrogenase 2 HAMAP MF_00487		PRO_0000113423
Regions
	Nucleotide binding	20 – 25	6	NAD By similarity
	Nucleotide binding	136 – 138	3	NAD By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	113	1	NAD By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67581-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 18E64807D16B7C96	FASTA	334	36,698
	10 20 30 40 50 60 MKGKLINSFL MKKPKISVIG AGKVGENVAY LLTILGLGDV YLFARYKKGL EPAKAKALDL 70 80 90 100 110 120 KQMAVLMDID INVKGISYDK EGFEELKGSD IVVITAGIPR REGMSREDLL YENLKILKKF 130 140 150 160 170 180 TDAIKEYAKD SIIIVVSNPV DTLTYATIKL TGFEPRRVIG MAGVLDSARF KNFVKEKIGI 190 200 210 220 230 240 SNADIRTLVL GTHGDLMVPV TSHSFIGDKP IEEVFSASEI DELIEKTRKG GAQIVSLMGT 250 260 270 280 290 300 SAYYAPAASV VIMVESIIND RKRVMPCSVY VEGEAAKHYE IEGVCIGLPV VLGKKGVEDF 310 320 330 ELVNLSGYEK RELLRSAKTL KEMVSLADKL LNEL

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07547.1.
PIR	D70444.
RefSeq	NP_214147.1.
3D structure databases
HSSP	HSSP built from PDB template 1GV0 based on UniProtKB P80039.
ModBase	Search...
Genome annotation databases
GeneID	1193297.
GenomeReviews	Gene locus aq_1665 in contig AE000657_GR.
KEGG	aae:aq_1665.
NMPDR	fig|224324.1.peg.1162.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67581.
OMA	O67581. EISANIR.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1665-MON.
BRENDA	1.1.1.37. 189781.
Family and domain databases
HAMAP	MF_00487. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
ProtoNet	Search...

Entry information

Entry name

MDH2_AQUAE

Accession

Primary (citable) accession number: O67581

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)