O67581 (MDH2_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase 2
EC=1.1.1.37

Gene names

Name:	mdh2
Ordered Locus Names:	aq_1665

Organism

Aquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Aquificae › Aquificae › Aquificales › Aquificaceae › Aquifex › Aquifex aeolicus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Malate dehydrogenase 2 HAMAP-Rule MF_00487		PRO_0000113423
Regions
	Nucleotide binding	20 – 25	6	NAD By similarity
	Nucleotide binding	136 – 138	3	NAD By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	113	1	NAD By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67581 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 18E64807D16B7C96	FASTA	334	36,698
	10 20 30 40 50 60 MKGKLINSFL MKKPKISVIG AGKVGENVAY LLTILGLGDV YLFARYKKGL EPAKAKALDL 70 80 90 100 110 120 KQMAVLMDID INVKGISYDK EGFEELKGSD IVVITAGIPR REGMSREDLL YENLKILKKF 130 140 150 160 170 180 TDAIKEYAKD SIIIVVSNPV DTLTYATIKL TGFEPRRVIG MAGVLDSARF KNFVKEKIGI 190 200 210 220 230 240 SNADIRTLVL GTHGDLMVPV TSHSFIGDKP IEEVFSASEI DELIEKTRKG GAQIVSLMGT 250 260 270 280 290 300 SAYYAPAASV VIMVESIIND RKRVMPCSVY VEGEAAKHYE IEGVCIGLPV VLGKKGVEDF 310 320 330 ELVNLSGYEK RELLRSAKTL KEMVSLADKL LNEL

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07547.1.
PIR	D70444.
RefSeq	NP_214147.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67581.
ModBase	Search...
Protein-protein interaction databases
STRING	224324.aq_1665.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC07547; AAC07547; aq_1665.
GeneID	1193297.
KEGG	aae:aq_1665.
PATRIC	20960352. VBIAquAeo85532_1288.
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	LMDIDIN.
Enzyme and pathway databases
BioCyc	AAEO224324:GJBH-1191-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH2_AQUAE

Accession

Primary (citable) accession number: O67581

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 1, 1998
Last modified:	May 29, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program