Reviewed, UniProtKB/Swiss-Prot O67556 (KPRS_AQUAE)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Ordered Locus Names:	aq_1636

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141105
Regions
	Region	212 – 225	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	128	1	Magnesium Potential
	Metal binding	130	1	Magnesium Potential
	Metal binding	139	1	Magnesium Potential
	Metal binding	143	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	O67556-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: F0355BAC6EE93E03	FASTA	311	33,722
	10 20 30 40 50 60 MNGVKIIAGT SNKTLSEEIA GYLGLRLTDA LITTFSDGEI RVQINESIRG YHVYVITSLS 70 80 90 100 110 120 NPVNHNLMEL LLTLDAVKRS SPKEITAVVP YYAYGRQDRQ DKPRTPISAK LVADLIQKAG 130 140 150 160 170 180 ANRVIVVDLH SPQIQGFFDI PVEHLTAIPV LYDYIRKNVV LENPIVVSPD AGGVKRARDL 190 200 210 220 230 240 ANKLGCGIGV ILKRRPEPNK AEVMDVVGDI EGKEAIIVDD IIDTAGTLVA AANLLVNKGV 250 260 270 280 290 300 KRVIACATHG IFSGPAVERL TNSPIEKVIV TNTLPVYEKK FGKLEVVSIA PLIGEAIRRI 310 QEGTSVSSLF T

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07513.1.
PIR	B70441.
RefSeq	NP_214121.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKU based on UniProtKB P14193.
ModBase	Search...
Genome annotation databases
GeneID	1193179.
GenomeReviews	Gene locus aq_1636 in contig AE000657_GR.
KEGG	aae:aq_1636.
NMPDR	fig|224324.1.peg.1136.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67556.
OMA	NVAEIMN.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1636-MON.
BRENDA	2.7.6.1. 189781.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_AQUAE

Accession

Primary (citable) accession number: O67556

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)