O67507 (CYSM_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine synthase
Short name=CSase
EC=2.5.1.47

Alternative name(s):
O-acetylserine sulfhydrylase
O-acetylserine (thiol)-lyase
Short name=OAS-TL

Gene names

Name:	cysM
Ordered Locus Names:	aq_1556

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Cysteine synthase		PRO_0000167106
Regions
	Region	200 – 204	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	95	1	Pyridoxal phosphate By similarity
	Binding site	282	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	65	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67507-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 54FC4338949FB40E	FASTA	327	36,155
	10 20 30 40 50 60 MSLKSCLMWV LGEHDEPYRK ARETILKLVG NTPLVRVKKC LPEDIPEDVE IWAKLESFNP 70 80 90 100 110 120 GGSVKDRPAL SMFLDALKRG LIKEGKVVID ATSGNTGIAL AMVGAALGVP VELAMPANVS 130 140 150 160 170 180 EERKKIIKAF GAKLYLTDPL EGTDGAILFV RELVQKYPEK YVYLDQYNNP ANWKAHFYST 190 200 210 220 230 240 GIEIWNQTKG RITHFVAGIG TGGTIMGTGR RLKIYNPNIQ VIGVQPAYPF HGIEGLKHIE 250 260 270 280 290 300 SSIKPGIFDE TFLDRTIFVE TEDAYYWARE LAKKDAIFVG QSSGAALWAC IQLARELAKK 310 320 GEKGVIVTVF PDGGEKYLTT ALFSDKD

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07459.1.
PIR	A70435.
RefSeq	NP_214072.1.
3D structure databases
ProteinModelPortal	O67507.
SMR	O67507. Positions 25-324.
ModBase	Search...
Genome annotation databases
GeneID	1193130.
GenomeReviews	Gene locus aq_1556 in contig AE000657_GR.
KEGG	aae:aq_1556.
NMPDR	fig|224324.1.peg.1087.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG748215.
OMA	KHIESSI.
PhylomeDB	O67507.
ProtClustDB	CLSK2299750.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1556-MONOMER.
BRENDA	2.5.1.47. 189781.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005858. CysM. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01136. cysKM. 1 hit. TIGR01138. cysM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSM_AQUAE

Accession

Primary (citable) accession number: O67507

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	August 10, 2010
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)