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O67505

- FABI_AQUAE

UniProt

O67505 - FABI_AQUAE

Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism By similarity.By similarity

    Catalytic activityi

    An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131NAD; via carbonyl oxygenBy similarity
    Binding sitei92 – 921NAD; via carbonyl oxygenBy similarity
    Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygenBy similarity
    Active sitei146 – 1461Proton acceptorBy similarity
    Active sitei156 – 1561Proton acceptorBy similarity
    Binding sitei163 – 1631NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 202NADBy similarity
    Nucleotide bindingi64 – 652NADBy similarity
    Nucleotide bindingi192 – 1965NADBy similarity

    GO - Molecular functioni

    1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

    GO - Biological processi

    1. fatty acid elongation Source: UniProtKB
    2. protein homotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1112-MONOMER.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
    Short name:
    ENR
    Alternative name(s):
    NADH-dependent enoyl-ACP reductase
    Gene namesi
    Name:fabI
    Ordered Locus Names:aq_1552
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 270270Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054894Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi224324.aq_1552.

    Structurei

    Secondary structure

    1
    270
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni3 – 64
    Beta strandi8 – 114
    Helixi20 – 3011
    Beta strandi34 – 418
    Helixi42 – 443
    Helixi45 – 5410
    Beta strandi60 – 623
    Helixi68 – 8114
    Beta strandi87 – 904
    Helixi97 – 1004
    Helixi104 – 1063
    Helixi109 – 11911
    Helixi121 – 13010
    Helixi131 – 1344
    Beta strandi140 – 1456
    Helixi147 – 1493
    Turni154 – 1574
    Helixi158 – 17821
    Turni179 – 1813
    Beta strandi183 – 1897
    Helixi203 – 21311
    Helixi222 – 23211
    Helixi235 – 2373
    Beta strandi244 – 2485
    Helixi251 – 2533

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2P91X-ray2.00A/B/C/D1-270[»]
    ProteinModelPortaliO67505.
    SMRiO67505. Positions 2-257.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO67505.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0623.
    KOiK00208.
    OMAiTGEIHHV.
    OrthoDBiEOG6HF644.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
    PRINTSiPR00081. GDHRDH.

    Sequencei

    Sequence statusi: Complete.

    O67505-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLLEGKRAL ITGVANERSI AYGIAKSFHR EGAQLAFTYA TPKLEKRVRE    50
    IAKGFGSDLV VKCDVSLDED IKNLKKFLEE NWGSLDIIVH SIAYAPKEEF 100
    KGGVIDTSRE GFKIAMDISV YSLIALTREL LPLMEGRNGA IVTLSYYGAE 150
    KVVPHYNVMG IAKAALESTV RYLAYDIAKH GHRINAISAG PVKTLAAYSI 200
    TGFHLLMEHT TKVNPFGKPI TIEDVGDTAV FLCSDWARAI TGEVVHVDNG 250
    YHIMGVFGRE EEIKKEVYGD 270
    Length:270
    Mass (Da):29,725
    Last modified:May 30, 2000 - v2
    Checksum:i2ACD6C85EE16DC68
    GO

    Sequence cautioni

    The sequence AAC07465.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07465.1. Different initiation.
    PIRiG70434.
    RefSeqiNP_214070.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07465; AAC07465; aq_1552.
    GeneIDi1193128.
    KEGGiaae:aq_1552.
    PATRICi20960182. VBIAquAeo85532_1204.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07465.1 . Different initiation.
    PIRi G70434.
    RefSeqi NP_214070.1. NC_000918.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2P91 X-ray 2.00 A/B/C/D 1-270 [» ]
    ProteinModelPortali O67505.
    SMRi O67505. Positions 2-257.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_1552.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07465 ; AAC07465 ; aq_1552 .
    GeneIDi 1193128.
    KEGGi aae:aq_1552.
    PATRICi 20960182. VBIAquAeo85532_1204.

    Phylogenomic databases

    eggNOGi COG0623.
    KOi K00208.
    OMAi TGEIHHV.
    OrthoDBi EOG6HF644.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci AAEO224324:GJBH-1112-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O67505.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
    PRINTSi PR00081. GDHRDH.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.
    2. "Crystal structure of enoyl-[acyl-carrier-protein] reductase (NADH) from Aquifex aeolicus VF5."
      Southeast collaboratory for structural genomics (SECSG)
      Submitted (APR-2007) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.

    Entry informationi

    Entry nameiFABI_AQUAE
    AccessioniPrimary (citable) accession number: O67505
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3