Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O67505

- FABI_AQUAE

UniProt

O67505 - FABI_AQUAE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygenBy similarity
Binding sitei92 – 921NAD; via carbonyl oxygenBy similarity
Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygenBy similarity
Active sitei146 – 1461Proton acceptorBy similarity
Active sitei156 – 1561Proton acceptorBy similarity
Binding sitei163 – 1631NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NADBy similarity
Nucleotide bindingi64 – 652NADBy similarity
Nucleotide bindingi192 – 1965NADBy similarity

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

GO - Biological processi

  1. fatty acid elongation Source: UniProtKB
  2. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1112-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:aq_1552
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054894Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi224324.aq_1552.

Structurei

Secondary structure

1
270
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni3 – 64Combined sources
Beta strandi8 – 114Combined sources
Helixi20 – 3011Combined sources
Beta strandi34 – 418Combined sources
Helixi42 – 443Combined sources
Helixi45 – 5410Combined sources
Beta strandi60 – 623Combined sources
Helixi68 – 8114Combined sources
Beta strandi87 – 904Combined sources
Helixi97 – 1004Combined sources
Helixi104 – 1063Combined sources
Helixi109 – 11911Combined sources
Helixi121 – 13010Combined sources
Helixi131 – 1344Combined sources
Beta strandi140 – 1456Combined sources
Helixi147 – 1493Combined sources
Turni154 – 1574Combined sources
Helixi158 – 17821Combined sources
Turni179 – 1813Combined sources
Beta strandi183 – 1897Combined sources
Helixi203 – 21311Combined sources
Helixi222 – 23211Combined sources
Helixi235 – 2373Combined sources
Beta strandi244 – 2485Combined sources
Helixi251 – 2533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P91X-ray2.00A/B/C/D1-270[»]
ProteinModelPortaliO67505.
SMRiO67505. Positions 2-257.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67505.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
InParanoidiO67505.
KOiK00208.
OMAiTGEIHHV.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

O67505-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLLEGKRAL ITGVANERSI AYGIAKSFHR EGAQLAFTYA TPKLEKRVRE
60 70 80 90 100
IAKGFGSDLV VKCDVSLDED IKNLKKFLEE NWGSLDIIVH SIAYAPKEEF
110 120 130 140 150
KGGVIDTSRE GFKIAMDISV YSLIALTREL LPLMEGRNGA IVTLSYYGAE
160 170 180 190 200
KVVPHYNVMG IAKAALESTV RYLAYDIAKH GHRINAISAG PVKTLAAYSI
210 220 230 240 250
TGFHLLMEHT TKVNPFGKPI TIEDVGDTAV FLCSDWARAI TGEVVHVDNG
260 270
YHIMGVFGRE EEIKKEVYGD
Length:270
Mass (Da):29,725
Last modified:May 30, 2000 - v2
Checksum:i2ACD6C85EE16DC68
GO

Sequence cautioni

The sequence AAC07465.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07465.1. Different initiation.
PIRiG70434.
RefSeqiNP_214070.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07465; AAC07465; aq_1552.
GeneIDi1193128.
KEGGiaae:aq_1552.
PATRICi20960182. VBIAquAeo85532_1204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07465.1 . Different initiation.
PIRi G70434.
RefSeqi NP_214070.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2P91 X-ray 2.00 A/B/C/D 1-270 [» ]
ProteinModelPortali O67505.
SMRi O67505. Positions 2-257.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_1552.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07465 ; AAC07465 ; aq_1552 .
GeneIDi 1193128.
KEGGi aae:aq_1552.
PATRICi 20960182. VBIAquAeo85532_1204.

Phylogenomic databases

eggNOGi COG0623.
InParanoidi O67505.
KOi K00208.
OMAi TGEIHHV.
OrthoDBi EOG6HF644.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci AAEO224324:GJBH-1112-MONOMER.

Miscellaneous databases

EvolutionaryTracei O67505.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSi PR00081. GDHRDH.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.
  2. "Crystal structure of enoyl-[acyl-carrier-protein] reductase (NADH) from Aquifex aeolicus VF5."
    Southeast collaboratory for structural genomics (SECSG)
    Submitted (APR-2007) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiFABI_AQUAE
AccessioniPrimary (citable) accession number: O67505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 26, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3