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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NAD; via carbonyl oxygenBy similarity1
Binding sitei92NAD; via carbonyl oxygenBy similarity1
Binding sitei95Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei146Proton acceptorBy similarity1
Active sitei156Proton acceptorBy similarity1
Binding sitei163NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 20NADBy similarity2
Nucleotide bindingi64 – 65NADBy similarity2
Nucleotide bindingi192 – 196NADBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:aq_1552
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000548941 – 270Enoyl-[acyl-carrier-protein] reductase [NADH] FabIAdd BLAST270

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi224324.aq_1552.

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni3 – 6Combined sources4
Beta strandi8 – 11Combined sources4
Helixi20 – 30Combined sources11
Beta strandi34 – 41Combined sources8
Helixi42 – 44Combined sources3
Helixi45 – 54Combined sources10
Beta strandi60 – 62Combined sources3
Helixi68 – 81Combined sources14
Beta strandi87 – 90Combined sources4
Helixi97 – 100Combined sources4
Helixi104 – 106Combined sources3
Helixi109 – 119Combined sources11
Helixi121 – 130Combined sources10
Helixi131 – 134Combined sources4
Beta strandi140 – 145Combined sources6
Helixi147 – 149Combined sources3
Turni154 – 157Combined sources4
Helixi158 – 178Combined sources21
Turni179 – 181Combined sources3
Beta strandi183 – 189Combined sources7
Helixi203 – 213Combined sources11
Helixi222 – 232Combined sources11
Helixi235 – 237Combined sources3
Beta strandi244 – 248Combined sources5
Helixi251 – 253Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P91X-ray2.00A/B/C/D1-270[»]
ProteinModelPortaliO67505.
SMRiO67505.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67505.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CSJ. Bacteria.
COG0623. LUCA.
InParanoidiO67505.
KOiK00208.
OMAiAGYCING.
OrthoDBiPOG091H06HU.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

O67505-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLEGKRAL ITGVANERSI AYGIAKSFHR EGAQLAFTYA TPKLEKRVRE
60 70 80 90 100
IAKGFGSDLV VKCDVSLDED IKNLKKFLEE NWGSLDIIVH SIAYAPKEEF
110 120 130 140 150
KGGVIDTSRE GFKIAMDISV YSLIALTREL LPLMEGRNGA IVTLSYYGAE
160 170 180 190 200
KVVPHYNVMG IAKAALESTV RYLAYDIAKH GHRINAISAG PVKTLAAYSI
210 220 230 240 250
TGFHLLMEHT TKVNPFGKPI TIEDVGDTAV FLCSDWARAI TGEVVHVDNG
260 270
YHIMGVFGRE EEIKKEVYGD
Length:270
Mass (Da):29,725
Last modified:May 30, 2000 - v2
Checksum:i2ACD6C85EE16DC68
GO

Sequence cautioni

The sequence AAC07465 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07465.1. Different initiation.
PIRiG70434.
RefSeqiNP_214070.1. NC_000918.1.
WP_010881008.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07465; AAC07465; aq_1552.
GeneIDi1193128.
KEGGiaae:aq_1552.
PATRICi20960182. VBIAquAeo85532_1204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07465.1. Different initiation.
PIRiG70434.
RefSeqiNP_214070.1. NC_000918.1.
WP_010881008.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P91X-ray2.00A/B/C/D1-270[»]
ProteinModelPortaliO67505.
SMRiO67505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07465; AAC07465; aq_1552.
GeneIDi1193128.
KEGGiaae:aq_1552.
PATRICi20960182. VBIAquAeo85532_1204.

Phylogenomic databases

eggNOGiENOG4105CSJ. Bacteria.
COG0623. LUCA.
InParanoidiO67505.
KOiK00208.
OMAiAGYCING.
OrthoDBiPOG091H06HU.

Enzyme and pathway databases

UniPathwayiUPA00094.

Miscellaneous databases

EvolutionaryTraceiO67505.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFABI_AQUAE
AccessioniPrimary (citable) accession number: O67505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 30, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.