O67501 (IPYR_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	aq_1547

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137475
Sites
	Metal binding	65	1	Magnesium 1 By similarity
	Metal binding	70	1	Magnesium 1 By similarity
	Metal binding	70	1	Magnesium 2 By similarity
	Metal binding	102	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67501 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 3F24C48904451327	FASTA	178	20,343
	10 20 30 40 50 60 MGYDQLPPGK NPPEDIYVVI EIPQGSAVKY ELDKDTGVIF VDRFLFTAMY YPFNYGFVPQ 70 80 90 100 110 120 TLADDGDPVD VLVISREPVV PGAVMRCRPI GMLEMRDEAG IDTKVIAVPH EKLDPSYSNI 130 140 150 160 170 KTVDNLPEIV REKIKHFFEH YKELEPGKWV KVENWKGLQD AIEEIKKGIE NYKKNKEG

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07463.1.
PIR	C70434.
RefSeq	NP_214066.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67501.
SMR	O67501. Positions 5-173.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193124.
GenomeReviews	Gene locus aq_1547 in contig AE000657_GR.
KEGG	aae:aq_1547.
NMPDR	fig|224324.1.peg.1081.
PATRIC	20960174. VBIAquAeo85532_1200.
Phylogenomic databases
HOGENOM	HBG529150.
OMA	AMVIMEE.
PhylomeDB	O67501.
ProtClustDB	PRK01250.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1547-MONOMER.
BRENDA	3.6.1.1. 396.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_AQUAE

Accession

Primary (citable) accession number: O67501

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program