O67480 (IDH_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP]
Short name=IDH
EC=1.1.1.42

Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase

Gene names

Name:	icd
Ordered Locus Names:	aq_1512

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Isocitrate dehydrogenase [NADP]		PRO_0000083548
Sites
	Metal binding	312	1	Magnesium or manganese By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Site	169	1	Critical for catalysis By similarity
	Site	239	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	123	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67480 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 06E47E36D844432A	FASTA	426	46,925
	10 20 30 40 50 60 MNKTTFENVY YWEGKAQIPQ EGQFIKLKED KTLEVPDNPI IPFIEGDGIG PEITQAMLLI 70 80 90 100 110 120 INTAVEKTYN GSKKIYWVEL LAGDKAEEKT GERLPQETLD VLKESIVGIK GPLGTPVGKG 130 140 150 160 170 180 VRSINSALRR AFDYYSAVRP VYWMGQATPI PNPERVDLVV FRENTDDVYA GVEFFAGTPE 190 200 210 220 230 240 AKKVREFLIK EMGAKEEGFP EDVGITVKPM SEFKTKRHVR KALRYALENN KKNVAVIGKG 250 260 270 280 290 300 NIMKATEGAF INWAFEVAEE PEFKGKVVTD PEAEPGEGQV KLTKVITDQM LMQLVLKPEA 310 320 330 340 350 360 WDVIIAQNLN GDYVSDLAAS LIGGPGFVPS GNIGDGYALF ESTHGTAWDI AGKGIANPLS 370 380 390 400 410 420 LTLSGAMMLE YIGWKEAAQK VYDAVRRTLA EHIGTPDIAS GFQKQGIEAK AVGTMEFAEE ISKRIE

References

[1]	"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07444.1.
PIR	F70431.
RefSeq	NP_214045.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67480.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1193103.
GenomeReviews	Gene locus aq_1512 in contig AE000657_GR.
KEGG	aae:aq_1512.
NMPDR	fig|224324.1.peg.1060.
PATRIC	20960134. VBIAquAeo85532_1180.
Phylogenomic databases
HOGENOM	HBG518924.
OMA	FQQIQTR.
PhylomeDB	O67480.
ProtClustDB	CLSK2299732.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1512-MONOMER.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004439. Isocitrate_DH_NADP_dimer_prok. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KO	K00031.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF1. NADP_IDH_prok. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH_AQUAE

Accession

Primary (citable) accession number: O67480

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program