Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O67475

- RIR2_AQUAE

UniProt

O67475 - RIR2_AQUAE

Protein

Ribonucleoside-diphosphate reductase subunit beta

Gene

nrdB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi97 – 971Iron 1PROSITE-ProRule annotation
    Metal bindingi127 – 1271Iron 1PROSITE-ProRule annotation
    Metal bindingi127 – 1271Iron 2By similarity
    Metal bindingi130 – 1301Iron 1PROSITE-ProRule annotation
    Active sitei134 – 1341PROSITE-ProRule annotation
    Metal bindingi194 – 1941Iron 2By similarity
    Metal bindingi228 – 2281Iron 2By similarity
    Metal bindingi577 – 5771Iron 2By similarity

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: UniProtKB-UniPathway
    3. intein-mediated protein splicing Source: InterPro
    4. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease, Oxidoreductase

    Keywords - Biological processi

    DNA replication, Intron homing

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1082-MONOMER.
    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    Cleaved into the following chain:
    Alternative name(s):
    Aae RIR2 intein
    Gene namesi
    Name:nrdB
    Ordered Locus Names:aq_1505
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 229229Ribonucleoside-diphosphate reductase subunit beta, 1st partSequence AnalysisPRO_0000030598Add
    BLAST
    Chaini230 – 575346Aae NrdB inteinSequence AnalysisPRO_0000030599Add
    BLAST
    Chaini576 – 696121Ribonucleoside-diphosphate reductase subunit beta, 2nd partSequence AnalysisPRO_0000030600Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.By similarity

    Protein-protein interaction databases

    STRINGi224324.aq_1505.

    Structurei

    3D structure databases

    ProteinModelPortaliO67475.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini377 – 507131DOD-type homing endonucleasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0208.
    KOiK00526.
    OMAiDEITEEW.
    OrthoDBiEOG6J48J7.

    Family and domain databases

    Gene3Di1.10.620.20. 4 hits.
    2.170.16.10. 1 hit.
    3.10.28.10. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR028992. Hedgehog/Intein_dom.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SMARTiSM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 2 hits.
    SSF51294. SSF51294. 2 hits.
    TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O67475-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKTEKNELV RKLIFNPQGD REASKRKIIK GNPTNIFELN EIKYSWAFDL    50
    YKLMGFTNFW IPEEIQMLED RKQYETVLSD YEKRAYELVL SFLIALDSFQ 100
    VDMLKEFGRM ITAPEVEMAI TAQEFQESVH AYSYQFILES VVDPVKADEI 150
    YNYWREDERL LERNKVIAEL YNEFIRKPNE ENFIKATIGN YILESLYFYS 200
    GFAFFYTLGR QGKMRNTVQQ IKYINRDELC FIEGTEVLTK RGFVDFRELR 250
    EDDLVAQYDI ETGEISWTKP YAYVERDYEG SMYRLKHPKS NWEVVATEGH 300
    EFIVRNLKTG KERKEPIEKV KLHPYSAIPV AGRYTGEVEE YDLWELVSGK 350
    GITLKTRSAV KNKLTPIEKL LIVLQADGTI DSKRNGKFTG FQQLKFFFSK 400
    YRKINEFEKI LNECAPYGIK WKKYERQDGI AYTVYYPNDL PIKPTKFFDE 450
    WVRLDEITEE WIREFVEELV KWDGHIPKDR NKKKVYYYST KEKRNKDFVQ 500
    ALCALGGMRT VVSRERNPKA KNPVYRIWIY LEDDYINTQT MVKEEFYYKG 550
    KVYCVSVPKG NIVVRYKDSV CIAGNCHVTL FRNIINTLRK ENPELFTPEI 600
    EKWIVEYFKY AVNEEIKWGQ YVTQNQILGI NDVLIERYIK YLGNLRITQI 650
    GFDPIYPEVT ENPLKWIDEF RKINNTKTDF FQAKPQTYSK ANELKW 696
    Length:696
    Mass (Da):82,931
    Last modified:August 1, 1998 - v1
    Checksum:iE5929D55C455A5EB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07431.1.
    PIRiA70431.
    RefSeqiNP_214040.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07431; AAC07431; aq_1505.
    GeneIDi1193098.
    KEGGiaae:aq_1505.
    PATRICi20960124. VBIAquAeo85532_1175.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07431.1 .
    PIRi A70431.
    RefSeqi NP_214040.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O67475.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_1505.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07431 ; AAC07431 ; aq_1505 .
    GeneIDi 1193098.
    KEGGi aae:aq_1505.
    PATRICi 20960124. VBIAquAeo85532_1175.

    Phylogenomic databases

    eggNOGi COG0208.
    KOi K00526.
    OMAi DEITEEW.
    OrthoDBi EOG6J48J7.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .
    BioCyci AAEO224324:GJBH-1082-MONOMER.

    Family and domain databases

    Gene3Di 1.10.620.20. 4 hits.
    2.170.16.10. 1 hit.
    3.10.28.10. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR028992. Hedgehog/Intein_dom.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SMARTi SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 2 hits.
    SSF51294. SSF51294. 2 hits.
    TIGRFAMsi TIGR01445. intein_Nterm. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiRIR2_AQUAE
    AccessioniPrimary (citable) accession number: O67475
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3