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Protein

Ribonucleoside-diphosphate reductase subunit beta

Gene

nrdB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi97Iron 1PROSITE-ProRule annotation1
Metal bindingi127Iron 1PROSITE-ProRule annotation1
Metal bindingi127Iron 2By similarity1
Metal bindingi130Iron 1PROSITE-ProRule annotation1
Active sitei134PROSITE-ProRule annotation1
Metal bindingi194Iron 2By similarity1
Metal bindingi228Iron 2By similarity1
Metal bindingi577Iron 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, Oxidoreductase
Biological processDNA replication, Intron homing
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-1076-MONOMER
UniPathwayiUPA00326

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Cleaved into the following chain:
Alternative name(s):
Aae RIR2 intein
Gene namesi
Name:nrdB
Ordered Locus Names:aq_1505
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000305981 – 229Ribonucleoside-diphosphate reductase subunit beta, 1st partSequence analysisAdd BLAST229
ChainiPRO_0000030599230 – 575Aae NrdB inteinSequence analysisAdd BLAST346
ChainiPRO_0000030600576 – 696Ribonucleoside-diphosphate reductase subunit beta, 2nd partSequence analysisAdd BLAST121

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiO67475

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi224324.aq_1505

Structurei

3D structure databases

ProteinModelPortaliO67475
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini377 – 507DOD-type homing endonucleasePROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105E05 Bacteria
COG0208 LUCA
COG1372 LUCA
InParanoidiO67475
KOiK00526
OrthoDBiPOG091H05VC

Family and domain databases

CDDicd01049 RNRR2, 1 hit
Gene3Di1.10.620.20, 2 hits
InterProiView protein in InterPro
IPR009078 Ferritin-like_SF
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR012348 RNR-like
IPR033909 RNR_small
IPR030475 RNR_small_AS
IPR000358 RNR_small_fam
PANTHERiPTHR23409 PTHR23409, 1 hit
PfamiView protein in Pfam
PF00268 Ribonuc_red_sm, 2 hits
SMARTiView protein in SMART
SM00306 HintN, 1 hit
SUPFAMiSSF47240 SSF47240, 2 hits
SSF51294 SSF51294, 2 hits
TIGRFAMsiTIGR01443 intein_Cterm, 1 hit
TIGR01445 intein_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 1 hit
PS50819 INTEIN_ENDONUCLEASE, 1 hit
PS50817 INTEIN_N_TER, 1 hit
PS00368 RIBORED_SMALL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O67475-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKTEKNELV RKLIFNPQGD REASKRKIIK GNPTNIFELN EIKYSWAFDL
60 70 80 90 100
YKLMGFTNFW IPEEIQMLED RKQYETVLSD YEKRAYELVL SFLIALDSFQ
110 120 130 140 150
VDMLKEFGRM ITAPEVEMAI TAQEFQESVH AYSYQFILES VVDPVKADEI
160 170 180 190 200
YNYWREDERL LERNKVIAEL YNEFIRKPNE ENFIKATIGN YILESLYFYS
210 220 230 240 250
GFAFFYTLGR QGKMRNTVQQ IKYINRDELC FIEGTEVLTK RGFVDFRELR
260 270 280 290 300
EDDLVAQYDI ETGEISWTKP YAYVERDYEG SMYRLKHPKS NWEVVATEGH
310 320 330 340 350
EFIVRNLKTG KERKEPIEKV KLHPYSAIPV AGRYTGEVEE YDLWELVSGK
360 370 380 390 400
GITLKTRSAV KNKLTPIEKL LIVLQADGTI DSKRNGKFTG FQQLKFFFSK
410 420 430 440 450
YRKINEFEKI LNECAPYGIK WKKYERQDGI AYTVYYPNDL PIKPTKFFDE
460 470 480 490 500
WVRLDEITEE WIREFVEELV KWDGHIPKDR NKKKVYYYST KEKRNKDFVQ
510 520 530 540 550
ALCALGGMRT VVSRERNPKA KNPVYRIWIY LEDDYINTQT MVKEEFYYKG
560 570 580 590 600
KVYCVSVPKG NIVVRYKDSV CIAGNCHVTL FRNIINTLRK ENPELFTPEI
610 620 630 640 650
EKWIVEYFKY AVNEEIKWGQ YVTQNQILGI NDVLIERYIK YLGNLRITQI
660 670 680 690
GFDPIYPEVT ENPLKWIDEF RKINNTKTDF FQAKPQTYSK ANELKW
Length:696
Mass (Da):82,931
Last modified:August 1, 1998 - v1
Checksum:iE5929D55C455A5EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC07431.1
PIRiA70431
RefSeqiNP_214040.1, NC_000918.1
WP_010880978.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC07431; AAC07431; aq_1505
GeneIDi1193098
KEGGiaae:aq_1505
PATRICifig|224324.8.peg.1175

Entry informationi

Entry nameiRIR2_AQUAE
AccessioniPrimary (citable) accession number: O67475
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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