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O67475

- RIR2_AQUAE

UniProt

O67475 - RIR2_AQUAE

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Protein

Ribonucleoside-diphosphate reductase subunit beta

Gene

nrdB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi97 – 971Iron 1PROSITE-ProRule annotation
Metal bindingi127 – 1271Iron 1PROSITE-ProRule annotation
Metal bindingi127 – 1271Iron 2By similarity
Metal bindingi130 – 1301Iron 1PROSITE-ProRule annotation
Active sitei134 – 1341PROSITE-ProRule annotation
Metal bindingi194 – 1941Iron 2By similarity
Metal bindingi228 – 2281Iron 2By similarity
Metal bindingi577 – 5771Iron 2By similarity

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-UniPathway
  3. intein-mediated protein splicing Source: InterPro
  4. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Oxidoreductase

Keywords - Biological processi

DNA replication, Intron homing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1082-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Cleaved into the following chain:
Alternative name(s):
Aae RIR2 intein
Gene namesi
Name:nrdB
Ordered Locus Names:aq_1505
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 229229Ribonucleoside-diphosphate reductase subunit beta, 1st partSequence AnalysisPRO_0000030598Add
BLAST
Chaini230 – 575346Aae NrdB inteinSequence AnalysisPRO_0000030599Add
BLAST
Chaini576 – 696121Ribonucleoside-diphosphate reductase subunit beta, 2nd partSequence AnalysisPRO_0000030600Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi224324.aq_1505.

Structurei

3D structure databases

ProteinModelPortaliO67475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini377 – 507131DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0208.
InParanoidiO67475.
KOiK00526.
OMAiDEITEEW.
OrthoDBiEOG6J48J7.

Family and domain databases

Gene3Di1.10.620.20. 4 hits.
2.170.16.10. 1 hit.
3.10.28.10. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SMARTiSM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 2 hits.
SSF51294. SSF51294. 2 hits.
TIGRFAMsiTIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O67475-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKTEKNELV RKLIFNPQGD REASKRKIIK GNPTNIFELN EIKYSWAFDL
60 70 80 90 100
YKLMGFTNFW IPEEIQMLED RKQYETVLSD YEKRAYELVL SFLIALDSFQ
110 120 130 140 150
VDMLKEFGRM ITAPEVEMAI TAQEFQESVH AYSYQFILES VVDPVKADEI
160 170 180 190 200
YNYWREDERL LERNKVIAEL YNEFIRKPNE ENFIKATIGN YILESLYFYS
210 220 230 240 250
GFAFFYTLGR QGKMRNTVQQ IKYINRDELC FIEGTEVLTK RGFVDFRELR
260 270 280 290 300
EDDLVAQYDI ETGEISWTKP YAYVERDYEG SMYRLKHPKS NWEVVATEGH
310 320 330 340 350
EFIVRNLKTG KERKEPIEKV KLHPYSAIPV AGRYTGEVEE YDLWELVSGK
360 370 380 390 400
GITLKTRSAV KNKLTPIEKL LIVLQADGTI DSKRNGKFTG FQQLKFFFSK
410 420 430 440 450
YRKINEFEKI LNECAPYGIK WKKYERQDGI AYTVYYPNDL PIKPTKFFDE
460 470 480 490 500
WVRLDEITEE WIREFVEELV KWDGHIPKDR NKKKVYYYST KEKRNKDFVQ
510 520 530 540 550
ALCALGGMRT VVSRERNPKA KNPVYRIWIY LEDDYINTQT MVKEEFYYKG
560 570 580 590 600
KVYCVSVPKG NIVVRYKDSV CIAGNCHVTL FRNIINTLRK ENPELFTPEI
610 620 630 640 650
EKWIVEYFKY AVNEEIKWGQ YVTQNQILGI NDVLIERYIK YLGNLRITQI
660 670 680 690
GFDPIYPEVT ENPLKWIDEF RKINNTKTDF FQAKPQTYSK ANELKW
Length:696
Mass (Da):82,931
Last modified:August 1, 1998 - v1
Checksum:iE5929D55C455A5EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07431.1.
PIRiA70431.
RefSeqiNP_214040.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07431; AAC07431; aq_1505.
GeneIDi1193098.
KEGGiaae:aq_1505.
PATRICi20960124. VBIAquAeo85532_1175.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07431.1 .
PIRi A70431.
RefSeqi NP_214040.1. NC_000918.1.

3D structure databases

ProteinModelPortali O67475.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_1505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07431 ; AAC07431 ; aq_1505 .
GeneIDi 1193098.
KEGGi aae:aq_1505.
PATRICi 20960124. VBIAquAeo85532_1175.

Phylogenomic databases

eggNOGi COG0208.
InParanoidi O67475.
KOi K00526.
OMAi DEITEEW.
OrthoDBi EOG6J48J7.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci AAEO224324:GJBH-1082-MONOMER.

Family and domain databases

Gene3Di 1.10.620.20. 4 hits.
2.170.16.10. 1 hit.
3.10.28.10. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
SMARTi SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 2 hits.
SSF51294. SSF51294. 2 hits.
TIGRFAMsi TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiRIR2_AQUAE
AccessioniPrimary (citable) accession number: O67475
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3