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O67475 (RIR2_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase subunit beta
EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase small subunit

Cleaved into the following chain:

  1. Aae NrdB intein
    Alternative name(s):
    Aae RIR2 intein
Gene names
Name:nrdB
Ordered Locus Names:aq_1505
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Aquificaceae › Aquifex ›

Protein attributes

Sequence length696 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 229229Ribonucleoside-diphosphate reductase subunit beta, 1st part Potential
PRO_0000030598
Chain230 – 575346Aae NrdB intein Potential
PRO_0000030599
Chain576 – 696121Ribonucleoside-diphosphate reductase subunit beta, 2nd part Potential
PRO_0000030600

Regions

Domain377 – 507131DOD-type homing endonuclease

Sites

Active site1341 By similarity
Metal binding971Iron 1 By similarity
Metal binding1271Iron 1 By similarity
Metal binding1271Iron 2 By similarity
Metal binding1301Iron 1 By similarity
Metal binding1941Iron 2 By similarity
Metal binding2281Iron 2 By similarity
Metal binding5771Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
O67475 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: E5929D55C455A5EB

FASTA69682,931
        10         20         30         40         50         60 
MEKTEKNELV RKLIFNPQGD REASKRKIIK GNPTNIFELN EIKYSWAFDL YKLMGFTNFW 

        70         80         90        100        110        120 
IPEEIQMLED RKQYETVLSD YEKRAYELVL SFLIALDSFQ VDMLKEFGRM ITAPEVEMAI 

       130        140        150        160        170        180 
TAQEFQESVH AYSYQFILES VVDPVKADEI YNYWREDERL LERNKVIAEL YNEFIRKPNE 

       190        200        210        220        230        240 
ENFIKATIGN YILESLYFYS GFAFFYTLGR QGKMRNTVQQ IKYINRDELC FIEGTEVLTK 

       250        260        270        280        290        300 
RGFVDFRELR EDDLVAQYDI ETGEISWTKP YAYVERDYEG SMYRLKHPKS NWEVVATEGH 

       310        320        330        340        350        360 
EFIVRNLKTG KERKEPIEKV KLHPYSAIPV AGRYTGEVEE YDLWELVSGK GITLKTRSAV 

       370        380        390        400        410        420 
KNKLTPIEKL LIVLQADGTI DSKRNGKFTG FQQLKFFFSK YRKINEFEKI LNECAPYGIK 

       430        440        450        460        470        480 
WKKYERQDGI AYTVYYPNDL PIKPTKFFDE WVRLDEITEE WIREFVEELV KWDGHIPKDR 

       490        500        510        520        530        540 
NKKKVYYYST KEKRNKDFVQ ALCALGGMRT VVSRERNPKA KNPVYRIWIY LEDDYINTQT 

       550        560        570        580        590        600 
MVKEEFYYKG KVYCVSVPKG NIVVRYKDSV CIAGNCHVTL FRNIINTLRK ENPELFTPEI 

       610        620        630        640        650        660 
EKWIVEYFKY AVNEEIKWGQ YVTQNQILGI NDVLIERYIK YLGNLRITQI GFDPIYPEVT 

       670        680        690 
ENPLKWIDEF RKINNTKTDF FQAKPQTYSK ANELKW

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000657 Genomic DNA. Translation: AAC07431.1.
PIRA70431.
RefSeqNP_214040.1. NC_000918.1.

3D structure databases

ProteinModelPortalO67475.
ModBaseSearch...

Protein-protein interaction databases

STRING224324.aq_1505.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC07431; AAC07431; aq_1505.
GeneID1193098.
KEGGaae:aq_1505.
PATRIC20960124. VBIAquAeo85532_1175.

Phylogenomic databases

eggNOGCOG0208.
KOK00526.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-1082-MONOMER.
UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 4 hits.
3.10.28.10. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SMARTSM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMsTIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR2_AQUAE
AccessionPrimary (citable) accession number: O67475
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: August 1, 1998
Last modified: May 29, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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