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Protein

DNA primase

Gene

dnaG

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+UniRule annotation1 PublicationNote: Binds 1 zinc ion per monomer.UniRule annotation1 Publication
  • Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi249Magnesium 1; catalyticUniRule annotation1
Metal bindingi293Magnesium 1; catalyticUniRule annotation1
Metal bindingi293Magnesium 2UniRule annotation1
Metal bindingi295Magnesium 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri35 – 59CHC2-typeUniRule annotationAdd BLAST25

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Transcription

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
Gene namesi
Name:dnaGUniRule annotation
Ordered Locus Names:aq_1493
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001804761 – 498DNA primaseAdd BLAST498

Interactioni

Subunit structurei

Monomer. Interacts with DnaB.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_1493.

Structurei

Secondary structure

1498
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 11Combined sources8
Helixi14 – 21Combined sources8
Beta strandi25 – 27Combined sources3
Beta strandi30 – 33Combined sources4
Beta strandi36 – 38Combined sources3
Beta strandi45 – 48Combined sources4
Turni49 – 52Combined sources4
Beta strandi53 – 56Combined sources4
Turni57 – 59Combined sources3
Helixi65 – 73Combined sources9
Helixi77 – 88Combined sources12
Helixi99 – 120Combined sources22
Helixi122 – 130Combined sources9
Helixi135 – 140Combined sources6
Helixi149 – 159Combined sources11
Helixi162 – 166Combined sources5
Turni167 – 169Combined sources3
Turni182 – 185Combined sources4
Beta strandi186 – 192Combined sources7
Beta strandi198 – 205Combined sources8
Beta strandi213 – 216Combined sources4
Helixi225 – 227Combined sources3
Helixi232 – 242Combined sources11
Beta strandi245 – 247Combined sources3
Helixi251 – 259Combined sources9
Beta strandi264 – 272Combined sources9
Helixi275 – 282Combined sources8
Beta strandi286 – 291Combined sources6
Helixi296 – 311Combined sources16
Beta strandi315 – 319Combined sources5
Helixi327 – 334Combined sources8
Helixi336 – 345Combined sources10
Helixi349 – 356Combined sources8
Helixi357 – 359Combined sources3
Helixi361 – 372Combined sources12
Helixi378 – 392Combined sources15
Helixi396 – 399Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AU3X-ray2.00A1-405[»]
ProteinModelPortaliO67465.
SMRiO67465.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67465.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini243 – 324ToprimUniRule annotationAdd BLAST82

Domaini

Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the DnaG primase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri35 – 59CHC2-typeUniRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C9G. Bacteria.
COG0358. LUCA.
HOGENOMiHOG000014483.
InParanoidiO67465.
KOiK02316.
OMAiIIICEGY.
OrthoDBiPOG091H0141.

Family and domain databases

Gene3Di3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPiMF_00974. DNA_primase_DnaG. 1 hit.
InterProiIPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR030846. DnaG_bac.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view]
PfamiPF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view]
SMARTiSM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01391. dnaG. 1 hit.
PROSITEiPS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDIDELRR EIDIVDVISE YLNLEKVGSN YRTNCPFHPD DTPSFYVSPS
60 70 80 90 100
KQIFKCFGCG VGGDAIKFVS LYEDISYFEA ALELAKRYGK KLDLEKISKD
110 120 130 140 150
EKVYVALDRV CDFYRESLLK NREASEYVKS RGIDPKVARK FDLGYAPSSE
160 170 180 190 200
ALVKVLKEND LLEAYLETKN LLSPTKGVYR DLFLRRVVIP IKDPRGRVIG
210 220 230 240 250
FGGRRIVEDK SPKYINSPDS RVFKKGENLF GLYEAKEYIK EEGFAILVEG
260 270 280 290 300
YFDLLRLFSE GIRNVVAPLG TALTQNQANL LSKFTKKVYI LYDGDDAGRK
310 320 330 340 350
AMKSAIPLLL SAGVEVYPVY LPEGYDPDEF IKEFGKEELR RLINSSGELF
360 370 380 390 400
ETLIKTAREN LEEKTREFRY YLGFISDGVR RFALASEFHT KYKVPMEILL
410 420 430 440 450
MKIEKNSQEK EIKLSFKEKI FLKGLIELKP KIDLEVLNLS PELKELAVNA
460 470 480 490
LNGEEHLLPK EVLEYQVDNL EKLFNNILRD LQKSGKKRKK RGLKNVNT
Length:498
Mass (Da):57,206
Last modified:August 1, 1998 - v1
Checksum:i941D95ECEF6E16D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07430.1.
PIRiG70429.
RefSeqiNP_214030.1. NC_000918.1.
WP_010880968.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07430; AAC07430; aq_1493.
GeneIDi1192996.
KEGGiaae:aq_1493.
PATRICi20960100. VBIAquAeo85532_1163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07430.1.
PIRiG70429.
RefSeqiNP_214030.1. NC_000918.1.
WP_010880968.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AU3X-ray2.00A1-405[»]
ProteinModelPortaliO67465.
SMRiO67465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1493.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07430; AAC07430; aq_1493.
GeneIDi1192996.
KEGGiaae:aq_1493.
PATRICi20960100. VBIAquAeo85532_1163.

Phylogenomic databases

eggNOGiENOG4105C9G. Bacteria.
COG0358. LUCA.
HOGENOMiHOG000014483.
InParanoidiO67465.
KOiK02316.
OMAiIIICEGY.
OrthoDBiPOG091H0141.

Miscellaneous databases

EvolutionaryTraceiO67465.

Family and domain databases

Gene3Di3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPiMF_00974. DNA_primase_DnaG. 1 hit.
InterProiIPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR030846. DnaG_bac.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view]
PfamiPF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view]
SMARTiSM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01391. dnaG. 1 hit.
PROSITEiPS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNAG_AQUAE
AccessioniPrimary (citable) accession number: O67465
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.