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Protein

DNA primase

Gene

dnaG

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+UniRule annotation1 PublicationNote: Binds 1 zinc ion per monomer.UniRule annotation1 Publication
  • Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi249 – 2491Magnesium 1; catalyticUniRule annotation
Metal bindingi293 – 2931Magnesium 1; catalyticUniRule annotation
Metal bindingi293 – 2931Magnesium 2UniRule annotation
Metal bindingi295 – 2951Magnesium 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri35 – 5925CHC2-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Transcription

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1072-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
Gene namesi
Name:dnaGUniRule annotation
Ordered Locus Names:aq_1493
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498DNA primasePRO_0000180476Add
BLAST

Interactioni

Subunit structurei

Monomer. Interacts with DnaB.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_1493.

Structurei

Secondary structure

1
498
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 118Combined sources
Helixi14 – 218Combined sources
Beta strandi25 – 273Combined sources
Beta strandi30 – 334Combined sources
Beta strandi36 – 383Combined sources
Beta strandi45 – 484Combined sources
Turni49 – 524Combined sources
Beta strandi53 – 564Combined sources
Turni57 – 593Combined sources
Helixi65 – 739Combined sources
Helixi77 – 8812Combined sources
Helixi99 – 12022Combined sources
Helixi122 – 1309Combined sources
Helixi135 – 1406Combined sources
Helixi149 – 15911Combined sources
Helixi162 – 1665Combined sources
Turni167 – 1693Combined sources
Turni182 – 1854Combined sources
Beta strandi186 – 1927Combined sources
Beta strandi198 – 2058Combined sources
Beta strandi213 – 2164Combined sources
Helixi225 – 2273Combined sources
Helixi232 – 24211Combined sources
Beta strandi245 – 2473Combined sources
Helixi251 – 2599Combined sources
Beta strandi264 – 2729Combined sources
Helixi275 – 2828Combined sources
Beta strandi286 – 2916Combined sources
Helixi296 – 31116Combined sources
Beta strandi315 – 3195Combined sources
Helixi327 – 3348Combined sources
Helixi336 – 34510Combined sources
Helixi349 – 3568Combined sources
Helixi357 – 3593Combined sources
Helixi361 – 37212Combined sources
Helixi378 – 39215Combined sources
Helixi396 – 3994Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AU3X-ray2.00A1-405[»]
ProteinModelPortaliO67465.
SMRiO67465. Positions 1-403.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67465.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini243 – 32482ToprimUniRule annotationAdd
BLAST

Domaini

Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the DnaG primase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri35 – 5925CHC2-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C9G. Bacteria.
COG0358. LUCA.
HOGENOMiHOG000014483.
InParanoidiO67465.
KOiK02316.
OMAiCPFHPDD.
OrthoDBiEOG6XDGTR.

Family and domain databases

Gene3Di3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPiMF_00974. DNA_primase_DnaG.
InterProiIPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR030846. DnaG_bac.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view]
PfamiPF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view]
SMARTiSM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01391. dnaG. 1 hit.
PROSITEiPS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDIDELRR EIDIVDVISE YLNLEKVGSN YRTNCPFHPD DTPSFYVSPS
60 70 80 90 100
KQIFKCFGCG VGGDAIKFVS LYEDISYFEA ALELAKRYGK KLDLEKISKD
110 120 130 140 150
EKVYVALDRV CDFYRESLLK NREASEYVKS RGIDPKVARK FDLGYAPSSE
160 170 180 190 200
ALVKVLKEND LLEAYLETKN LLSPTKGVYR DLFLRRVVIP IKDPRGRVIG
210 220 230 240 250
FGGRRIVEDK SPKYINSPDS RVFKKGENLF GLYEAKEYIK EEGFAILVEG
260 270 280 290 300
YFDLLRLFSE GIRNVVAPLG TALTQNQANL LSKFTKKVYI LYDGDDAGRK
310 320 330 340 350
AMKSAIPLLL SAGVEVYPVY LPEGYDPDEF IKEFGKEELR RLINSSGELF
360 370 380 390 400
ETLIKTAREN LEEKTREFRY YLGFISDGVR RFALASEFHT KYKVPMEILL
410 420 430 440 450
MKIEKNSQEK EIKLSFKEKI FLKGLIELKP KIDLEVLNLS PELKELAVNA
460 470 480 490
LNGEEHLLPK EVLEYQVDNL EKLFNNILRD LQKSGKKRKK RGLKNVNT
Length:498
Mass (Da):57,206
Last modified:August 1, 1998 - v1
Checksum:i941D95ECEF6E16D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07430.1.
PIRiG70429.
RefSeqiNP_214030.1. NC_000918.1.
WP_010880968.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07430; AAC07430; aq_1493.
GeneIDi1192996.
KEGGiaae:aq_1493.
PATRICi20960100. VBIAquAeo85532_1163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07430.1.
PIRiG70429.
RefSeqiNP_214030.1. NC_000918.1.
WP_010880968.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AU3X-ray2.00A1-405[»]
ProteinModelPortaliO67465.
SMRiO67465. Positions 1-403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1493.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07430; AAC07430; aq_1493.
GeneIDi1192996.
KEGGiaae:aq_1493.
PATRICi20960100. VBIAquAeo85532_1163.

Phylogenomic databases

eggNOGiENOG4105C9G. Bacteria.
COG0358. LUCA.
HOGENOMiHOG000014483.
InParanoidiO67465.
KOiK02316.
OMAiCPFHPDD.
OrthoDBiEOG6XDGTR.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1072-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO67465.

Family and domain databases

Gene3Di3.90.580.10. 1 hit.
3.90.980.10. 1 hit.
HAMAPiMF_00974. DNA_primase_DnaG.
InterProiIPR013264. DNA_primase_core_N.
IPR006295. DNA_primase_DnaG.
IPR030846. DnaG_bac.
IPR006171. Toprim_domain.
IPR002694. Znf_CHC2.
[Graphical view]
PfamiPF08275. Toprim_N. 1 hit.
PF01807. zf-CHC2. 1 hit.
[Graphical view]
SMARTiSM00493. TOPRIM. 1 hit.
SM00400. ZnF_CHCC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01391. dnaG. 1 hit.
PROSITEiPS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.
  2. "Crosstalk between primase subunits can act to regulate primer synthesis in trans."
    Corn J.E., Pease P.J., Hura G.L., Berger J.M.
    Mol. Cell 20:391-401(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1-405 IN COMPLEX WITH ZINC, COFACTOR.

Entry informationi

Entry nameiDNAG_AQUAE
AccessioniPrimary (citable) accession number: O67465
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: May 11, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.