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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates guanosine-37 in various tRNAs.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei115 – 1151S-adenosyl-L-methionine; via amide nitrogenBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1070-MONOMER.
BRENDAi2.1.1.228. 396.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferase (EC:2.1.1.228)
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene namesi
Name:trmD
Ordered Locus Names:aq_1489
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257tRNA (guanine-N(1)-)-methyltransferasePRO_0000060317Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224324.aq_1489.

Structurei

Secondary structure

1
257
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 138Combined sources
Helixi15 – 217Combined sources
Helixi25 – 328Combined sources
Beta strandi35 – 428Combined sources
Helixi43 – 464Combined sources
Beta strandi56 – 583Combined sources
Helixi67 – 8115Combined sources
Beta strandi85 – 895Combined sources
Helixi98 – 1058Combined sources
Beta strandi108 – 1147Combined sources
Helixi122 – 1276Combined sources
Beta strandi140 – 1423Combined sources
Helixi143 – 15513Combined sources
Turni158 – 1603Combined sources
Beta strandi186 – 1883Combined sources
Helixi195 – 1973Combined sources
Beta strandi201 – 2033Combined sources
Turni204 – 2063Combined sources
Helixi207 – 2137Combined sources
Turni214 – 2163Combined sources
Helixi217 – 2193Combined sources
Helixi228 – 2303Combined sources
Turni231 – 2344Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OY5X-ray2.60A/B/C1-257[»]
ProteinModelPortaliO67463.
SMRiO67463. Positions 4-235.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67463.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 1396S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.Curated

Phylogenomic databases

eggNOGiENOG4105D6X. Bacteria.
COG0336. LUCA.
HOGENOMiHOG000016242.
InParanoidiO67463.
KOiK00554.
OMAiPWTEESI.
OrthoDBiPOG091H01WE.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

O67463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNPLRFFV LTIFPHIISC YSEYGIVKQA IKKGKVEVYP IDLREFAPKG
60 70 80 90 100
QVDDVPYGGL PGMVLKPEPI YEAYDYVVEN YGKPFVLITE PWGEKLNQKL
110 120 130 140 150
VNELSKKERI MIICGRYEGV DERVKKIVDM EISLGDFILS GGEIVALAVI
160 170 180 190 200
DAVSRVLPGV LSEPQSIQED SFQNRWLGYP VYTRPREYRG MKVPEELLSG
210 220 230 240 250
HHKLIELWKL WHRIENTVKK RPDLIPKDLT ELEKDILNSI LSGKSFKEWL

KEHKHLL
Length:257
Mass (Da):29,652
Last modified:August 1, 1998 - v1
Checksum:iF03ACF8EE2D7EBD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07418.1.
PIRiE70429.
RefSeqiNP_214028.1. NC_000918.1.
WP_010880966.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07418; AAC07418; aq_1489.
GeneIDi1192994.
KEGGiaae:aq_1489.
PATRICi20960096. VBIAquAeo85532_1161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07418.1.
PIRiE70429.
RefSeqiNP_214028.1. NC_000918.1.
WP_010880966.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OY5X-ray2.60A/B/C1-257[»]
ProteinModelPortaliO67463.
SMRiO67463. Positions 4-235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1489.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07418; AAC07418; aq_1489.
GeneIDi1192994.
KEGGiaae:aq_1489.
PATRICi20960096. VBIAquAeo85532_1161.

Phylogenomic databases

eggNOGiENOG4105D6X. Bacteria.
COG0336. LUCA.
HOGENOMiHOG000016242.
InParanoidiO67463.
KOiK00554.
OMAiPWTEESI.
OrthoDBiPOG091H01WE.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1070-MONOMER.
BRENDAi2.1.1.228. 396.

Miscellaneous databases

EvolutionaryTraceiO67463.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMD_AQUAE
AccessioniPrimary (citable) accession number: O67463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.