ID   METF_AQUAE              Reviewed;         296 AA.
AC   O67422;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   16-JUN-2009, entry version 51.
DE   RecName: Full=5,10-methylenetetrahydrofolate reductase;
DE            EC=1.5.1.20;
GN   Name=metF; OrderedLocusNames=aq_1429;
OS   Aquifex aeolicus.
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=63363;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   MEDLINE=98196666; PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex
RT   aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- CATALYTIC ACTIVITY: 5-methyltetrahydrofolate + NAD(P)(+) = 5,10-
CC       methylenetetrahydrofolate + NAD(P)H.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate pathway.
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase
CC       family.
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DR   EMBL; AE000657; AAC07387.1; -; Genomic_DNA.
DR   PIR; D70424; D70424.
DR   RefSeq; NP_213987.1; -.
DR   HSSP; P00394; 1B5T.
DR   GeneID; 1192953; -.
DR   GenomeReviews; AE000657_GR; aq_1429.
DR   KEGG; aae:aq_1429; -.
DR   NMPDR; fig|224324.1.peg.1002; -.
DR   HOGENOM; O67422; -.
DR   OMA; O67422; FFPPKTP.
DR   BioCyc; AAEO224324:AQ_1429-MON; -.
DR   BRENDA; 1.5.1.20; 189781.
DR   GO; GO:0004489; F:methylenetetrahydrofolate reductase (NADPH)...; IEA:EC.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR003171; Mehydrof_redctse.
DR   InterPro; IPR004620; MTHF_reductase_bac.
DR   Pfam; PF02219; MTHFR; 1.
DR   TIGRFAMs; TIGR00676; fadh2; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Complete proteome; FAD; Flavoprotein;
KW   Methionine biosynthesis; NAD; NADP; Oxidoreductase.
FT   CHAIN         1    296       5,10-methylenetetrahydrofolate reductase.
FT                                /FTId=PRO_0000190257.
SQ   SEQUENCE   296 AA;  33871 MW;  E497CC6CAC9FAFC0 CRC64;
     MKIGDILRKG VFSISFEFFP PKTEEGERQL FETIRKLEKL NPTFVSVTYG AGGSTRDRTR
     NIVQKIHEET NLTVMAHLTC IAHTREELID ILQDYKNIGI ENILALRGDV PRDKPDWRPP
     KGACKYAKEL VELIRKEFGD WFSIGVASYP EGHPESPNLE WEVKYFKEKV EAGADFSITQ
     MFFVNDYYYR FVEMCKNAGI DISIIPGIMP ITNFKQIRKF ASLCGATIPQ SLIEKLEKVE
     DKPEEVKKIG IEFAINQCLD LIEHGVPGLH FYTLNKSDAT LKIYEAIKDK IPARST
//