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O67422 (METF_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5,10-methylenetetrahydrofolate reductase

EC=1.5.1.20
Gene names
Name:metF
Ordered Locus Names:aq_1429
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteriaAquificaeAquificalesAquificaceaeAquifex

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD By similarity.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Sequence similarities

Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2962965,10-methylenetetrahydrofolate reductase
PRO_0000190257

Regions

Nucleotide binding17 – 226NAD By similarity
Nucleotide binding48 – 514FAD By similarity
Nucleotide binding48 – 492NAD By similarity
Nucleotide binding107 – 1093FAD By similarity
Nucleotide binding126 – 1272FAD By similarity
Nucleotide binding153 – 1564FAD By similarity

Sites

Active site171Proton donor/acceptor By similarity
Binding site771FAD By similarity
Binding site1091Substrate By similarity
Binding site1491FAD By similarity
Binding site1801Substrate By similarity
Binding site2161Substrate By similarity
Binding site2201Substrate By similarity
Binding site2721Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O67422 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: E497CC6CAC9FAFC0

FASTA29633,871
        10         20         30         40         50         60 
MKIGDILRKG VFSISFEFFP PKTEEGERQL FETIRKLEKL NPTFVSVTYG AGGSTRDRTR 

        70         80         90        100        110        120 
NIVQKIHEET NLTVMAHLTC IAHTREELID ILQDYKNIGI ENILALRGDV PRDKPDWRPP 

       130        140        150        160        170        180 
KGACKYAKEL VELIRKEFGD WFSIGVASYP EGHPESPNLE WEVKYFKEKV EAGADFSITQ 

       190        200        210        220        230        240 
MFFVNDYYYR FVEMCKNAGI DISIIPGIMP ITNFKQIRKF ASLCGATIPQ SLIEKLEKVE 

       250        260        270        280        290 
DKPEEVKKIG IEFAINQCLD LIEHGVPGLH FYTLNKSDAT LKIYEAIKDK IPARST 

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000657 Genomic DNA. Translation: AAC07387.1.
PIRD70424.
RefSeqNP_213987.1. NC_000918.1.

3D structure databases

ProteinModelPortalO67422.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224324.aq_1429.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC07387; AAC07387; aq_1429.
GeneID1192953.
KEGGaae:aq_1429.
PATRIC20960008. VBIAquAeo85532_1118.

Phylogenomic databases

eggNOGCOG0685.
HOGENOMHOG000246233.
KOK00297.
OMAFIRAETG.
OrthoDBEOG6D2KVW.
ProtClustDBCLSK2299700.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-1028-MONOMER.
UniPathwayUPA00193.

Family and domain databases

InterProIPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
[Graphical view]
TIGRFAMsTIGR00676. fadh2. 1 hit.
TIGR00677. fadh2_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMETF_AQUAE
AccessionPrimary (citable) accession number: O67422
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: December 11, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways