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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei17Proton donor/acceptorBy similarity1
Binding sitei77FADBy similarity1
Binding sitei109SubstrateBy similarity1
Binding sitei149FADBy similarity1
Binding sitei180SubstrateBy similarity1
Binding sitei216SubstrateBy similarity1
Binding sitei220SubstrateBy similarity1
Binding sitei272SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 22NADBy similarity6
Nucleotide bindingi48 – 51FADBy similarity4
Nucleotide bindingi48 – 49NADBy similarity2
Nucleotide bindingi107 – 109FADBy similarity3
Nucleotide bindingi126 – 127FADBy similarity2
Nucleotide bindingi153 – 156FADBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BRENDAi1.5.1.20. 396.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:aq_1429
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902571 – 2965,10-methylenetetrahydrofolate reductaseAdd BLAST296

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_1429.

Structurei

3D structure databases

ProteinModelPortaliO67422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105SYT. Bacteria.
COG0685. LUCA.
HOGENOMiHOG000246233.
InParanoidiO67422.
KOiK00297.
OMAiEMHPQAR.
OrthoDBiPOG091H02HI.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
TIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

O67422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGDILRKG VFSISFEFFP PKTEEGERQL FETIRKLEKL NPTFVSVTYG
60 70 80 90 100
AGGSTRDRTR NIVQKIHEET NLTVMAHLTC IAHTREELID ILQDYKNIGI
110 120 130 140 150
ENILALRGDV PRDKPDWRPP KGACKYAKEL VELIRKEFGD WFSIGVASYP
160 170 180 190 200
EGHPESPNLE WEVKYFKEKV EAGADFSITQ MFFVNDYYYR FVEMCKNAGI
210 220 230 240 250
DISIIPGIMP ITNFKQIRKF ASLCGATIPQ SLIEKLEKVE DKPEEVKKIG
260 270 280 290
IEFAINQCLD LIEHGVPGLH FYTLNKSDAT LKIYEAIKDK IPARST
Length:296
Mass (Da):33,871
Last modified:August 1, 1998 - v1
Checksum:iE497CC6CAC9FAFC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07387.1.
PIRiD70424.
RefSeqiNP_213987.1. NC_000918.1.
WP_010880925.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07387; AAC07387; aq_1429.
GeneIDi1192953.
KEGGiaae:aq_1429.
PATRICi20960008. VBIAquAeo85532_1118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07387.1.
PIRiD70424.
RefSeqiNP_213987.1. NC_000918.1.
WP_010880925.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1429.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07387; AAC07387; aq_1429.
GeneIDi1192953.
KEGGiaae:aq_1429.
PATRICi20960008. VBIAquAeo85532_1118.

Phylogenomic databases

eggNOGiENOG4105SYT. Bacteria.
COG0685. LUCA.
HOGENOMiHOG000246233.
InParanoidiO67422.
KOiK00297.
OMAiEMHPQAR.
OrthoDBiPOG091H02HI.

Enzyme and pathway databases

UniPathwayiUPA00193.
BRENDAi1.5.1.20. 396.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
TIGR00677. fadh2_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETF_AQUAE
AccessioniPrimary (citable) accession number: O67422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.