O67422 (METF_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydrofolate reductase
EC=1.5.1.20

Gene names

Name:	metF
Ordered Locus Names:	aq_1429

Organism

Aquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Aquificae › Aquificae › Aquificales › Aquificaceae › Aquifex › Aquifex aeolicus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	5,10-methylenetetrahydrofolate reductase		PRO_0000190257

Sequences

	Sequence		Length	Mass (Da)
	O67422 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: E497CC6CAC9FAFC0	FASTA	296	33,871
	10 20 30 40 50 60 MKIGDILRKG VFSISFEFFP PKTEEGERQL FETIRKLEKL NPTFVSVTYG AGGSTRDRTR 70 80 90 100 110 120 NIVQKIHEET NLTVMAHLTC IAHTREELID ILQDYKNIGI ENILALRGDV PRDKPDWRPP 130 140 150 160 170 180 KGACKYAKEL VELIRKEFGD WFSIGVASYP EGHPESPNLE WEVKYFKEKV EAGADFSITQ 190 200 210 220 230 240 MFFVNDYYYR FVEMCKNAGI DISIIPGIMP ITNFKQIRKF ASLCGATIPQ SLIEKLEKVE 250 260 270 280 290 DKPEEVKKIG IEFAINQCLD LIEHGVPGLH FYTLNKSDAT LKIYEAIKDK IPARST

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07387.1.
PIR	D70424.
RefSeq	NP_213987.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67422.
ModBase	Search...
Protein-protein interaction databases
STRING	224324.aq_1429.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC07387; AAC07387; aq_1429.
GeneID	1192953.
KEGG	aae:aq_1429.
PATRIC	20960008. VBIAquAeo85532_1118.
Phylogenomic databases
eggNOG	COG0685.
HOGENOM	HOG000246233.
KO	K00297.
OMA	SITQMFF.
ProtClustDB	CLSK2299700.
Enzyme and pathway databases
BioCyc	AAEO224324:GJBH-1028-MONOMER.
UniPathway	UPA00193.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit. TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_AQUAE

Accession

Primary (citable) accession number: O67422

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program