Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O67422

- METF_AQUAE

UniProt

O67422 - METF_AQUAE

Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei17 – 171Proton donor/acceptorBy similarity
    Binding sitei77 – 771FADBy similarity
    Binding sitei109 – 1091SubstrateBy similarity
    Binding sitei149 – 1491FADBy similarity
    Binding sitei180 – 1801SubstrateBy similarity
    Binding sitei216 – 2161SubstrateBy similarity
    Binding sitei220 – 2201SubstrateBy similarity
    Binding sitei272 – 2721SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi17 – 226NADBy similarity
    Nucleotide bindingi48 – 514FADBy similarity
    Nucleotide bindingi48 – 492NADBy similarity
    Nucleotide bindingi107 – 1093FADBy similarity
    Nucleotide bindingi126 – 1272FADBy similarity
    Nucleotide bindingi153 – 1564FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-1028-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:metF
    Ordered Locus Names:aq_1429
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    ProteomesiUP000000798: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2962965,10-methylenetetrahydrofolate reductasePRO_0000190257Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi224324.aq_1429.

    Structurei

    3D structure databases

    ProteinModelPortaliO67422.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246233.
    KOiK00297.
    OMAiFEFFPTK.
    OrthoDBiEOG6D2KVW.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00676. fadh2. 1 hit.
    TIGR00677. fadh2_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O67422-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIGDILRKG VFSISFEFFP PKTEEGERQL FETIRKLEKL NPTFVSVTYG    50
    AGGSTRDRTR NIVQKIHEET NLTVMAHLTC IAHTREELID ILQDYKNIGI 100
    ENILALRGDV PRDKPDWRPP KGACKYAKEL VELIRKEFGD WFSIGVASYP 150
    EGHPESPNLE WEVKYFKEKV EAGADFSITQ MFFVNDYYYR FVEMCKNAGI 200
    DISIIPGIMP ITNFKQIRKF ASLCGATIPQ SLIEKLEKVE DKPEEVKKIG 250
    IEFAINQCLD LIEHGVPGLH FYTLNKSDAT LKIYEAIKDK IPARST 296
    Length:296
    Mass (Da):33,871
    Last modified:August 1, 1998 - v1
    Checksum:iE497CC6CAC9FAFC0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07387.1.
    PIRiD70424.
    RefSeqiNP_213987.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07387; AAC07387; aq_1429.
    GeneIDi1192953.
    KEGGiaae:aq_1429.
    PATRICi20960008. VBIAquAeo85532_1118.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07387.1 .
    PIRi D70424.
    RefSeqi NP_213987.1. NC_000918.1.

    3D structure databases

    ProteinModelPortali O67422.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224324.aq_1429.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC07387 ; AAC07387 ; aq_1429 .
    GeneIDi 1192953.
    KEGGi aae:aq_1429.
    PATRICi 20960008. VBIAquAeo85532_1118.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246233.
    KOi K00297.
    OMAi FEFFPTK.
    OrthoDBi EOG6D2KVW.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci AAEO224324:GJBH-1028-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00676. fadh2. 1 hit.
    TIGR00677. fadh2_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.

    Entry informationi

    Entry nameiMETF_AQUAE
    AccessioniPrimary (citable) accession number: O67422
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3