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O67422

- METF_AQUAE

UniProt

O67422 - METF_AQUAE

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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei17 – 171Proton donor/acceptorBy similarity
Binding sitei77 – 771FADBy similarity
Binding sitei109 – 1091SubstrateBy similarity
Binding sitei149 – 1491FADBy similarity
Binding sitei180 – 1801SubstrateBy similarity
Binding sitei216 – 2161SubstrateBy similarity
Binding sitei220 – 2201SubstrateBy similarity
Binding sitei272 – 2721SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 226NADBy similarity
Nucleotide bindingi48 – 514FADBy similarity
Nucleotide bindingi48 – 492NADBy similarity
Nucleotide bindingi107 – 1093FADBy similarity
Nucleotide bindingi126 – 1272FADBy similarity
Nucleotide bindingi153 – 1564FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1028-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:aq_1429
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2962965,10-methylenetetrahydrofolate reductasePRO_0000190257Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_1429.

Structurei

3D structure databases

ProteinModelPortaliO67422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246233.
InParanoidiO67422.
KOiK00297.
OMAiFEFFPTK.
OrthoDBiEOG6D2KVW.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
TIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

O67422-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIGDILRKG VFSISFEFFP PKTEEGERQL FETIRKLEKL NPTFVSVTYG
60 70 80 90 100
AGGSTRDRTR NIVQKIHEET NLTVMAHLTC IAHTREELID ILQDYKNIGI
110 120 130 140 150
ENILALRGDV PRDKPDWRPP KGACKYAKEL VELIRKEFGD WFSIGVASYP
160 170 180 190 200
EGHPESPNLE WEVKYFKEKV EAGADFSITQ MFFVNDYYYR FVEMCKNAGI
210 220 230 240 250
DISIIPGIMP ITNFKQIRKF ASLCGATIPQ SLIEKLEKVE DKPEEVKKIG
260 270 280 290
IEFAINQCLD LIEHGVPGLH FYTLNKSDAT LKIYEAIKDK IPARST
Length:296
Mass (Da):33,871
Last modified:August 1, 1998 - v1
Checksum:iE497CC6CAC9FAFC0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07387.1.
PIRiD70424.
RefSeqiNP_213987.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07387; AAC07387; aq_1429.
GeneIDi1192953.
KEGGiaae:aq_1429.
PATRICi20960008. VBIAquAeo85532_1118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07387.1 .
PIRi D70424.
RefSeqi NP_213987.1. NC_000918.1.

3D structure databases

ProteinModelPortali O67422.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224324.aq_1429.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07387 ; AAC07387 ; aq_1429 .
GeneIDi 1192953.
KEGGi aae:aq_1429.
PATRICi 20960008. VBIAquAeo85532_1118.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246233.
InParanoidi O67422.
KOi K00297.
OMAi FEFFPTK.
OrthoDBi EOG6D2KVW.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci AAEO224324:GJBH-1028-MONOMER.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00676. fadh2. 1 hit.
TIGR00677. fadh2_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiMETF_AQUAE
AccessioniPrimary (citable) accession number: O67422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3