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Protein

Valine--tRNA ligase

Gene

valS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.UniRule annotation

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei803ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-1010-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligaseUniRule annotation (EC:6.1.1.9UniRule annotation)
Alternative name(s):
Valyl-tRNA synthetaseUniRule annotation
Short name:
ValRSUniRule annotation
Gene namesi
Name:valSUniRule annotation
Ordered Locus Names:aq_1413
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001062111 – 1165Valine--tRNA ligaseAdd BLAST1165

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_1413

Structurei

3D structure databases

ProteinModelPortaliO67411
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1001 – 1032UniRule annotationAdd BLAST32
Coiled coili1097 – 1165UniRule annotationAdd BLAST69

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 53"HIGH" regionAdd BLAST11
Motifi800 – 804"KMSKS" region5

Domaini

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.UniRule annotation
The C-terminal coiled-coil domain is crucial for aminoacylation activity.UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105CA4 Bacteria
COG0525 LUCA
HOGENOMiHOG000020093
InParanoidiO67411
KOiK01873
OMAiGSRDDFM
OrthoDBiPOG091H01HY

Family and domain databases

CDDicd07962 Anticodon_Ia_Val, 1 hit
Gene3Di1.10.287.380, 1 hit
3.40.50.620, 3 hits
3.90.740.10, 1 hit
HAMAPiMF_02004 Val_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033705 Anticodon_Ia_Val
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR010978 tRNA-bd_arm
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR037118 Val-tRNA_synth_C_sf
IPR019499 Val-tRNA_synth_tRNA-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR002303 Valyl-tRNA_ligase
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 3 hits
PF10458 Val_tRNA-synt_C, 1 hit
PRINTSiPR00986 TRNASYNTHVAL
SUPFAMiSSF46589 SSF46589, 1 hit
SSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

O67411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIKEYNPRE IEEKWSRYWI EKALYHVEKP DKRKKFSVVI PPPNVTGSLH
60 70 80 90 100
MGHALNATLQ DVIVRWQRMR GRECVWVPGF DHAGIATQYV VEKQLAKEGK
110 120 130 140 150
SRLELGREEF LKKVWEWVPK SRNAIRTQLT KLGVSVDWKR ERFTLDEGFS
160 170 180 190 200
RAVRKAFRTL FEEGLIYRAE YIVNWCPNDR TALSDLEVEY EEEKGSLWYI
210 220 230 240 250
KYPIVDENGK DTGEFITVAT TRPETMLGDT AVAVNPNDDR YKHLVGKRAR
260 270 280 290 300
LPLVNWERKD LRGNSVSNLI PIIADERVKM DFGTGAVKIT PAHDPLDFEI
310 320 330 340 350
GKTHDLPFVR VIDETGRMNE NAGDFAGMDR YEAREAIVAK LREDELLEKE
360 370 380 390 400
EEITHSVGHC YRCKTVIEPM VSVQWFVKVS DPRIKDISIK VVEEGIKERE
410 420 430 440 450
EKELFVQLAQ VEELSVVIPL KEEGRHKIVV VLDKTAKFLV GKREGELAYI
460 470 480 490 500
YYPKGDERTL EFAKRLAEEF KDYEFMVAYE GLPKIVVMGN EQLVEPGSYL
510 520 530 540 550
FVYDGKKLDL YKLEGEKPKF LKTLGKGEAD LEVTQKSIQI KPERIKKVEE
560 570 580 590 600
REKRVKFIPE HWKKFYLDWM YNLKDWCISR QIWWGHRIPV WYCKDCGNVN
610 620 630 640 650
VFTDDDFDRA HDKIIFNLIA DGKIDQEFTP EEVEKVLKSP HFVHPEMTVL
660 670 680 690 700
DFYKKFVFHR YYNMDITADS LRLLFTQDMN PMAMLTPGVS TRNIYKYDSQ
710 720 730 740 750
KKKWKMVLRC KKCGSENLEQ ERDVLDTWFS SALWPFGVFG WPESTEDLKN
760 770 780 790 800
LYPTDLLVTG FDIIFFWVAR MIMMGTHFMK DIPFYDVYVH ALVRDKYGRK
810 820 830 840 850
MSKTIGNVID PLDIIERYGA DALRFTLAIL TVQGRDIKLA EEKFEGYKHF
860 870 880 890 900
ANKIWNAARY VLMNTPEDFI ARIPYMAPLK PEDKWIITKL NETAEEVNKA
910 920 930 940 950
LENYQYSQAA HAIYEFFWSD YCDWYIEFTK ERIYKKAPED NEEEKAKVEN
960 970 980 990 1000
ERTTALYTLH YVLEKALRIL HPFMPYITEE LWHKLPNAEG ESISLAEFPQ
1010 1020 1030 1040 1050
KNEDEIYEED KQKVERLKEI ISAIRAIRSD LQIKPSEKIK ASFKTESEFS
1060 1070 1080 1090 1100
RRVIQEFKNH ILNLAKLESF EEVSQRPQNT VATFSKDTEI YVHIEGHVDL
1110 1120 1130 1140 1150
DKLIQSYEKK REKLLKELER VNKKLSNENF LKKAPVEVVE KEKQIKEELE
1160
NDLKKVEEIL KVLRS
Length:1,165
Mass (Da):137,151
Last modified:August 1, 1998 - v1
Checksum:iE612D4F28EF4A237
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC07375.1
PIRiA70423
RefSeqiNP_213976.1, NC_000918.1
WP_010880914.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC07375; AAC07375; aq_1413
GeneIDi1192942
KEGGiaae:aq_1413
PATRICifig|224324.8.peg.1107

Similar proteinsi

Entry informationi

Entry nameiSYV_AQUAE
AccessioniPrimary (citable) accession number: O67411
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: March 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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