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Protein

Valine--tRNA ligase

Gene

valS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.UniRule annotation

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei803 – 8031ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1017-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligaseUniRule annotation (EC:6.1.1.9UniRule annotation)
Alternative name(s):
Valyl-tRNA synthetaseUniRule annotation
Short name:
ValRSUniRule annotation
Gene namesi
Name:valSUniRule annotation
Ordered Locus Names:aq_1413
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11651165Valine--tRNA ligasePRO_0000106211Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224324.aq_1413.

Structurei

3D structure databases

ProteinModelPortaliO67411.
SMRiO67411. Positions 551-601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1001 – 103232UniRule annotationAdd
BLAST
Coiled coili1097 – 116569UniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi800 – 8045"KMSKS" region

Domaini

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.UniRule annotation
The C-terminal coiled-coil domain is crucial for aminoacylation activity.UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105CA4. Bacteria.
COG0525. LUCA.
HOGENOMiHOG000020093.
InParanoidiO67411.
KOiK01873.
OMAiHRIPAYA.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.287.380. 1 hit.
1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 4 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIKEYNPRE IEEKWSRYWI EKALYHVEKP DKRKKFSVVI PPPNVTGSLH
60 70 80 90 100
MGHALNATLQ DVIVRWQRMR GRECVWVPGF DHAGIATQYV VEKQLAKEGK
110 120 130 140 150
SRLELGREEF LKKVWEWVPK SRNAIRTQLT KLGVSVDWKR ERFTLDEGFS
160 170 180 190 200
RAVRKAFRTL FEEGLIYRAE YIVNWCPNDR TALSDLEVEY EEEKGSLWYI
210 220 230 240 250
KYPIVDENGK DTGEFITVAT TRPETMLGDT AVAVNPNDDR YKHLVGKRAR
260 270 280 290 300
LPLVNWERKD LRGNSVSNLI PIIADERVKM DFGTGAVKIT PAHDPLDFEI
310 320 330 340 350
GKTHDLPFVR VIDETGRMNE NAGDFAGMDR YEAREAIVAK LREDELLEKE
360 370 380 390 400
EEITHSVGHC YRCKTVIEPM VSVQWFVKVS DPRIKDISIK VVEEGIKERE
410 420 430 440 450
EKELFVQLAQ VEELSVVIPL KEEGRHKIVV VLDKTAKFLV GKREGELAYI
460 470 480 490 500
YYPKGDERTL EFAKRLAEEF KDYEFMVAYE GLPKIVVMGN EQLVEPGSYL
510 520 530 540 550
FVYDGKKLDL YKLEGEKPKF LKTLGKGEAD LEVTQKSIQI KPERIKKVEE
560 570 580 590 600
REKRVKFIPE HWKKFYLDWM YNLKDWCISR QIWWGHRIPV WYCKDCGNVN
610 620 630 640 650
VFTDDDFDRA HDKIIFNLIA DGKIDQEFTP EEVEKVLKSP HFVHPEMTVL
660 670 680 690 700
DFYKKFVFHR YYNMDITADS LRLLFTQDMN PMAMLTPGVS TRNIYKYDSQ
710 720 730 740 750
KKKWKMVLRC KKCGSENLEQ ERDVLDTWFS SALWPFGVFG WPESTEDLKN
760 770 780 790 800
LYPTDLLVTG FDIIFFWVAR MIMMGTHFMK DIPFYDVYVH ALVRDKYGRK
810 820 830 840 850
MSKTIGNVID PLDIIERYGA DALRFTLAIL TVQGRDIKLA EEKFEGYKHF
860 870 880 890 900
ANKIWNAARY VLMNTPEDFI ARIPYMAPLK PEDKWIITKL NETAEEVNKA
910 920 930 940 950
LENYQYSQAA HAIYEFFWSD YCDWYIEFTK ERIYKKAPED NEEEKAKVEN
960 970 980 990 1000
ERTTALYTLH YVLEKALRIL HPFMPYITEE LWHKLPNAEG ESISLAEFPQ
1010 1020 1030 1040 1050
KNEDEIYEED KQKVERLKEI ISAIRAIRSD LQIKPSEKIK ASFKTESEFS
1060 1070 1080 1090 1100
RRVIQEFKNH ILNLAKLESF EEVSQRPQNT VATFSKDTEI YVHIEGHVDL
1110 1120 1130 1140 1150
DKLIQSYEKK REKLLKELER VNKKLSNENF LKKAPVEVVE KEKQIKEELE
1160
NDLKKVEEIL KVLRS
Length:1,165
Mass (Da):137,151
Last modified:August 1, 1998 - v1
Checksum:iE612D4F28EF4A237
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07375.1.
PIRiA70423.
RefSeqiNP_213976.1. NC_000918.1.
WP_010880914.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07375; AAC07375; aq_1413.
GeneIDi1192942.
KEGGiaae:aq_1413.
PATRICi20959986. VBIAquAeo85532_1107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07375.1.
PIRiA70423.
RefSeqiNP_213976.1. NC_000918.1.
WP_010880914.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67411.
SMRiO67411. Positions 551-601.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1413.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07375; AAC07375; aq_1413.
GeneIDi1192942.
KEGGiaae:aq_1413.
PATRICi20959986. VBIAquAeo85532_1107.

Phylogenomic databases

eggNOGiENOG4105CA4. Bacteria.
COG0525. LUCA.
HOGENOMiHOG000020093.
InParanoidiO67411.
KOiK01873.
OMAiHRIPAYA.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1017-MONOMER.

Family and domain databases

Gene3Di1.10.287.380. 1 hit.
1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERiPTHR11946:SF5. PTHR11946:SF5. 4 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF46589. SSF46589. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiSYV_AQUAE
AccessioniPrimary (citable) accession number: O67411
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 17, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.