Reviewed, UniProtKB/Swiss-Prot O67398 (DNLI_AQUAE)

Names and origin

Protein names

Recommended name:
    Probable DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	aq_1394

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 584	584	Probable DNA ligase HAMAP MF_00407		PRO_0000059623
Sites
	Active site	250	1	N6-AMP-lysine intermediate By similarity
	Binding site	248	1	ATP By similarity
	Binding site	255	1	ATP By similarity
	Binding site	270	1	ATP By similarity
	Binding site	299	1	ATP By similarity
	Binding site	339	1	ATP By similarity
	Binding site	416	1	ATP By similarity
	Binding site	422	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67398-1 [UniParc]. Last modified June 20, 2001. Version 2. Checksum: CB96A7451E9BDC1D	FASTA	584	66,945
	10 20 30 40 50 60 MEYRILAEFY ERIEKTTSRI EMVSSLVELF KQTPKELIDK VVYLSIGKIA PEYTGLDYNF 70 80 90 100 110 120 GEKLAIRALS RVLKIPALEI EKRVRQEGDL GEAGRKLYEE LGFKPEGTLT VEEVYNGLLN 130 140 150 160 170 180 IAKAVGIGSQ ERKISIFASL LKKATPLEAK YLLRTITERL RLGIGDNTIL EALSIAFTGS 190 200 210 220 230 240 SVNREVVERA YNLTSDLGYV AKILAEKGLE GVKQVKIQVG RPVRPMLAER MSSPILILRK 250 260 270 280 290 300 LGGKCGAEYK YDGERIQAHR KGDEFYLFSR RLENITHQYP DLIEFLKEAI PHDFIVELEA 310 320 330 340 350 360 VVIDPASGEI RPFQELMHRK VKYVTKYHIT KYPVAGFLFD IIYLDGEDLT LKPYPERREI 370 380 390 400 410 420 LEKVVKRTDR IGLVPRKIVD NVEDLENFFY QAIEEGCEGL VCKSLAPNSI YQAGKRGFLW 430 440 450 460 470 480 IKYKRDYKSV LADTLDLVVV GGFYGKGQRK GTFGSLLMAC YDPESDMFKT VTKVGTGFTE 490 500 510 520 530 540 DDFKKLEEIL MPRKLNHRHP RVNSILEADM WFEPYLVLEI TGAELTLSPV HTCGWGKVSP 550 560 570 580 NRGIGLRFPR FTGRYRFDKR PEDATTEQEI IEMYRMQRKI RVRS

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07362.1. Different initiation.
PIR	D70421.
RefSeq	NP_213963.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1192929.
GenomeReviews	Gene locus aq_1394 in contig AE000657_GR.
KEGG	aae:aq_1394.
NMPDR	fig|224324.1.peg.978.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67398.
OMA	O67398. RNWLKLK.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1394-MON.
BRENDA	6.5.1.1. 189781.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_AQUAE

Accession

Primary (citable) accession number: O67398

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	June 20, 2001
Last modified:	May 5, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)