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Protein

Probable DNA ligase

Gene

lig

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei248 – 2481ATPUniRule annotation
Active sitei250 – 2501N6-AMP-lysine intermediateUniRule annotation
Binding sitei255 – 2551ATPUniRule annotation
Binding sitei270 – 2701ATPUniRule annotation
Binding sitei299 – 2991ATPUniRule annotation
Binding sitei339 – 3391ATPUniRule annotation
Binding sitei416 – 4161ATPUniRule annotation
Binding sitei422 – 4221ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: GO_Central
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation Source: GO_Central
  5. DNA ligation involved in DNA repair Source: InterPro
  6. DNA recombination Source: UniProtKB-HAMAP
  7. lagging strand elongation Source: GO_Central
  8. nucleotide-excision repair Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1004-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:aq_1394
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584Probable DNA ligasePRO_0000059623Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_1394.

Structurei

3D structure databases

ProteinModelPortaliO67398.
SMRiO67398. Positions 1-579.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
InParanoidiO67398.
KOiK10747.
OMAiPQVVIEV.
OrthoDBiEOG6Q8HXT.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67398-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYRILAEFY ERIEKTTSRI EMVSSLVELF KQTPKELIDK VVYLSIGKIA
60 70 80 90 100
PEYTGLDYNF GEKLAIRALS RVLKIPALEI EKRVRQEGDL GEAGRKLYEE
110 120 130 140 150
LGFKPEGTLT VEEVYNGLLN IAKAVGIGSQ ERKISIFASL LKKATPLEAK
160 170 180 190 200
YLLRTITERL RLGIGDNTIL EALSIAFTGS SVNREVVERA YNLTSDLGYV
210 220 230 240 250
AKILAEKGLE GVKQVKIQVG RPVRPMLAER MSSPILILRK LGGKCGAEYK
260 270 280 290 300
YDGERIQAHR KGDEFYLFSR RLENITHQYP DLIEFLKEAI PHDFIVELEA
310 320 330 340 350
VVIDPASGEI RPFQELMHRK VKYVTKYHIT KYPVAGFLFD IIYLDGEDLT
360 370 380 390 400
LKPYPERREI LEKVVKRTDR IGLVPRKIVD NVEDLENFFY QAIEEGCEGL
410 420 430 440 450
VCKSLAPNSI YQAGKRGFLW IKYKRDYKSV LADTLDLVVV GGFYGKGQRK
460 470 480 490 500
GTFGSLLMAC YDPESDMFKT VTKVGTGFTE DDFKKLEEIL MPRKLNHRHP
510 520 530 540 550
RVNSILEADM WFEPYLVLEI TGAELTLSPV HTCGWGKVSP NRGIGLRFPR
560 570 580
FTGRYRFDKR PEDATTEQEI IEMYRMQRKI RVRS
Length:584
Mass (Da):66,945
Last modified:June 20, 2001 - v2
Checksum:iCB96A7451E9BDC1D
GO

Sequence cautioni

The sequence AAC07362.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07362.1. Different initiation.
PIRiD70421.
RefSeqiNP_213963.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07362; AAC07362; aq_1394.
GeneIDi1192929.
KEGGiaae:aq_1394.
PATRICi20959956. VBIAquAeo85532_1092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07362.1. Different initiation.
PIRiD70421.
RefSeqiNP_213963.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67398.
SMRiO67398. Positions 1-579.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07362; AAC07362; aq_1394.
GeneIDi1192929.
KEGGiaae:aq_1394.
PATRICi20959956. VBIAquAeo85532_1092.

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
InParanoidiO67398.
KOiK10747.
OMAiPQVVIEV.
OrthoDBiEOG6Q8HXT.

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-1004-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiDNLI_AQUAE
AccessioniPrimary (citable) accession number: O67398
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: February 4, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.