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O67398 (DNLI_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable DNA ligase
EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:aq_1394
OrganismAquifex aeolicus (strain VF5) [Reference proteome] [HAMAP]
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Aquificaceae › Aquifex ›

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence caution

The sequence AAC07362.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 584584Probable DNA ligase HAMAP-Rule MF_00407
PRO_0000059623

Sites

Active site2501N6-AMP-lysine intermediate By similarity
Binding site2481ATP By similarity
Binding site2551ATP By similarity
Binding site2701ATP By similarity
Binding site2991ATP By similarity
Binding site3391ATP By similarity
Binding site4161ATP By similarity
Binding site4221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O67398 [UniParc].

Last modified June 20, 2001. Version 2.
Checksum: CB96A7451E9BDC1D

FASTA58466,945
        10         20         30         40         50         60 
MEYRILAEFY ERIEKTTSRI EMVSSLVELF KQTPKELIDK VVYLSIGKIA PEYTGLDYNF 

        70         80         90        100        110        120 
GEKLAIRALS RVLKIPALEI EKRVRQEGDL GEAGRKLYEE LGFKPEGTLT VEEVYNGLLN 

       130        140        150        160        170        180 
IAKAVGIGSQ ERKISIFASL LKKATPLEAK YLLRTITERL RLGIGDNTIL EALSIAFTGS 

       190        200        210        220        230        240 
SVNREVVERA YNLTSDLGYV AKILAEKGLE GVKQVKIQVG RPVRPMLAER MSSPILILRK 

       250        260        270        280        290        300 
LGGKCGAEYK YDGERIQAHR KGDEFYLFSR RLENITHQYP DLIEFLKEAI PHDFIVELEA 

       310        320        330        340        350        360 
VVIDPASGEI RPFQELMHRK VKYVTKYHIT KYPVAGFLFD IIYLDGEDLT LKPYPERREI 

       370        380        390        400        410        420 
LEKVVKRTDR IGLVPRKIVD NVEDLENFFY QAIEEGCEGL VCKSLAPNSI YQAGKRGFLW 

       430        440        450        460        470        480 
IKYKRDYKSV LADTLDLVVV GGFYGKGQRK GTFGSLLMAC YDPESDMFKT VTKVGTGFTE 

       490        500        510        520        530        540 
DDFKKLEEIL MPRKLNHRHP RVNSILEADM WFEPYLVLEI TGAELTLSPV HTCGWGKVSP 

       550        560        570        580 
NRGIGLRFPR FTGRYRFDKR PEDATTEQEI IEMYRMQRKI RVRS

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000657 Genomic DNA. Translation: AAC07362.1. Different initiation.
PIRD70421.
RefSeqNP_213963.1. NC_000918.1.

3D structure databases

ProteinModelPortalO67398.
SMRO67398. Positions 1-579.
ModBaseSearch...

Protein-protein interaction databases

STRING224324.aq_1394.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC07362; AAC07362; aq_1394.
GeneID1192929.
KEGGaae:aq_1394.
PATRIC20959956. VBIAquAeo85532_1092.

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAAQVACLY.
ProtClustDBPRK01109.

Enzyme and pathway databases

BioCycAAEO224324:GJBH-1004-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. False negative.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_AQUAE
AccessionPrimary (citable) accession number: O67398
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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