O67388 (NUOK2_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit K 2
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit K 2
NDH-1 subunit K 2

Gene names

Name:	nuoK2
Ordered Locus Names:	aq_1378

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	102 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01456
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01456
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01456.
Sequence similarities	Belongs to the complex I subunit 4L family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 102	102	NADH-quinone oxidoreductase subunit K 2 HAMAP MF_01456		PRO_0000389931
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	30 – 50	21	Helical; Potential
	Transmembrane	66 – 86	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	O67388 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: CCE6159F4CC4A7F4	FASTA	102	10,905
	10 20 30 40 50 60 MKTIPLEAFL TVSMILFGLG LIGIIARRNL VTVLMSLELA LNAVNIALVG ADHYLGLAEG 70 80 90 100 QIFALFIIAL AATEAAVGLG IIIAIFRLKK VESTDEIREL RG

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07351.1.
PIR	A70420.
RefSeq	NP_213952.1. NC_000918.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1192918.
GenomeReviews	Gene locus aq_1378 in contig AE000657_GR.
KEGG	aae:aq_1378.
NMPDR	fig|224324.1.peg.967.
PATRIC	20959930. VBIAquAeo85532_1079.
Phylogenomic databases
HOGENOM	HBG673066.
OMA	IPMEHGL.
PhylomeDB	O67388.
ProtClustDB	CLSK2748450.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1378-MONOMER.
Family and domain databases
HAMAP	MF_01456. NDH1_NuoK. [Tree]
InterPro	IPR001133. NADH_UbQ_OxRdtase_chain4L/K. [Graphical view]
KO	K00340.
PANTHER	PTHR11434. Oxidored_4L. 1 hit.
Pfam	PF00420. Oxidored_q2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOK2_AQUAE

Accession

Primary (citable) accession number: O67388

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2009
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program