O67373 (MURC_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	aq_1360

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182046
Regions
	Nucleotide binding	113 – 119	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O67373 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 12665EE14448232C	FASTA	454	50,893
	10 20 30 40 50 60 MLRERIKKFH FIGIGGIGMS GIAQILLEMG YKVSGSDISE NKNTKLLKQK GAKIYIGHRP 70 80 90 100 110 120 ENLGDAQVVV YSSAVKPDNP EIQEAKRRNI PVIPRGEMLA ELFKLKEGIA VSGSHGKTTT 130 140 150 160 170 180 TSMIAEILIN AGLEPTVIIG GRLKRLGTNA KLGRGELLVS EADESDGSFL KLQPAVAVIT 190 200 210 220 230 240 NVDKEHLDFY ENFERVKEAF EQFMNSVPFY GFAVVNLDDP TLAQLVKKSH ERVITYGINS 250 260 270 280 290 300 PALVRAKNLY LKEGRYEFGV EFKGKELGRI HLGIAGIHNV YNALAATGVA LELGVSFEVI 310 320 330 340 350 360 KKSLEEFRNA ERRLELKGYY KNSPVYDDYG HHPTEIKAVI NSLRDMYPDK NLLVVFQPHR 370 380 390 400 410 420 YSRTYYLFED FVKVLKDIDK LIVTDIYPAS ENNVYGVSAE ELARKSGAVF AKDKEEVFEK 430 440 450 VREVHDEGDV ILFLGAGSIS KWCEEFLKEV NLEK

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07323.1.
PIR	B70418.
RefSeq	NP_213937.1. NC_000918.1.
3D structure databases
ProteinModelPortal	O67373.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1192811.
GenomeReviews	Gene locus aq_1360 in contig AE000657_GR.
KEGG	aae:aq_1360.
NMPDR	fig|224324.1.peg.952.
PATRIC	20959896. VBIAquAeo85532_1064.
Phylogenomic databases
HOGENOM	HBG476594.
OMA	YQPHRYS.
PhylomeDB	O67373.
ProtClustDB	CLSK2299677.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1360-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_AQUAE

Accession

Primary (citable) accession number: O67373

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program