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O67368

- LIPA_AQUAE

UniProt

O67368 - LIPA_AQUAE

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Protein
Lipoyl synthase
Gene
lipA, aq_1355
Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi27 – 271Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi32 – 321Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi38 – 381Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi53 – 531Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi57 – 571Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi60 – 601Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAAEO224324:GJBH-971-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:aq_1355
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
ProteomesiUP000000798: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Lipoyl synthaseUniRule annotation
PRO_0000102284Add
BLAST

Proteomic databases

PRIDEiO67368.

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_1355.

Structurei

3D structure databases

ProteinModelPortaliO67368.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

O67368-1 [UniParc]FASTAAdd to Basket

« Hide

MMKPVLHFSK LYEVKKLLRK SRLYTVCEES RCPNISECFG NKTATFMILG    50
NRCTRRCAFC NVEKGFPKGV DPEEPYRLLE AVKTLGLKYV VITSVTRDDL 100
PDGGASHFAK CIRVLKENIE DIKVEVLIPD FRGNKKALEV VLKEKPVVLN 150
HNVETVPRLY PSVRIGANYK RSLNILKWSK EIDKSVYTKS ALILGFGERK 200
EEVIKVMEDL RSVDCDFLVL GQYYQPSLKH HPVVKYYSEE EFKEFEEIGY 250
EMGFKFVVSK PNARSSYKAF ESLLST 276
Length:276
Mass (Da):31,785
Last modified:August 1, 1998 - v1
Checksum:i4AA328191ED486E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07325.1.
PIRiE70417.
RefSeqiNP_213932.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07325; AAC07325; aq_1355.
GeneIDi1192806.
KEGGiaae:aq_1355.
PATRICi20959886. VBIAquAeo85532_1059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000657 Genomic DNA. Translation: AAC07325.1 .
PIRi E70417.
RefSeqi NP_213932.1. NC_000918.1.

3D structure databases

ProteinModelPortali O67368.
ModBasei Search...

Protein-protein interaction databases

STRINGi 224324.aq_1355.

Proteomic databases

PRIDEi O67368.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC07325 ; AAC07325 ; aq_1355 .
GeneIDi 1192806.
KEGGi aae:aq_1355.
PATRICi 20959886. VBIAquAeo85532_1059.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci AAEO224324:GJBH-971-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VF5.

Entry informationi

Entry nameiLIPA_AQUAE
AccessioniPrimary (citable) accession number: O67368
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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