O67342 (NUON1_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit N 1
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit N 1
NDH-1 subunit N 1

Gene names

Name:	nuoN1
Ordered Locus Names:	aq_1322

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_00445
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_00445
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00445.
Sequence similarities	Belongs to the complex I subunit 2 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	NADH-quinone oxidoreductase subunit N 1 HAMAP MF_00445		PRO_0000391098
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	65 – 85	21	Helical; Potential
	Transmembrane	98 – 118	21	Helical; Potential
	Transmembrane	122 – 142	21	Helical; Potential
	Transmembrane	155 – 175	21	Helical; Potential
	Transmembrane	192 – 212	21	Helical; Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Transmembrane	259 – 279	21	Helical; Potential
	Transmembrane	285 – 305	21	Helical; Potential
	Transmembrane	312 – 332	21	Helical; Potential
	Transmembrane	356 – 376	21	Helical; Potential
	Transmembrane	401 – 421	21	Helical; Potential
	Transmembrane	436 – 456	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	O67342 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5C72D12A83E8FF26	FASTA	464	51,286
	10 20 30 40 50 60 MNWNAILPEA ILAIGILTVF ILELFLERKH YKFLSVLAFI FVVLSGYSIF FVNYPAKLFF 70 80 90 100 110 120 DGFSVDALNL IGKLFILAVT GFVLLSSYDY FSKKNSQYGE LPYLYLIATL GLMVMISSDN 130 140 150 160 170 180 LAIIFTGLEL ASITMYILVG LFRREYLSKE GAFKYLVIGT TGTSMYALGS ALVYASSGSM 190 200 210 220 230 240 VLSPVKEENT LFALGVILII SALALKVSAV PFHFWTPDAY EGAPTPTTAY LSTVPKIGMY 250 260 270 280 290 300 FLFVKLTMYL FSAFPDWKYV VMLLAVLSMF YGNIVAYAQK SVKRLLAYSS IAHAGYFLTA 310 320 330 340 350 360 LTAVDKHLFS ALLFYVFVYA LATVGAFTVL AILEKKEGWT HHFLDFKGLK EENPVLASML 370 380 390 400 410 420 ALFLFALIGI PPAAVFLGKL GIFFGLVKTD MFALGILFAI ASLISAGYYL KVIVYMFLYS 430 440 450 460 GEVRHGQTTV SAGEAFTVLG TAFLVIFFGL FPHVVLDFIL RALS

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07305.1.
PIR	C70414.
RefSeq	NP_213906.1. NC_000918.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1192780.
GenomeReviews	Gene locus aq_1322 in contig AE000657_GR.
KEGG	aae:aq_1322.
NMPDR	fig|224324.1.peg.921.
PATRIC	20959826. VBIAquAeo85532_1030.
Phylogenomic databases
HOGENOM	HBG747830.
OMA	MSSEALM.
PhylomeDB	O67342.
ProtClustDB	CLSK2299661.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1322-MONOMER.
Family and domain databases
HAMAP	MF_00445. NDH1_NuoN_1. [Tree]
InterPro	IPR010096. NADH-Q_OxRdtase_suN/2. IPR001750. NADH_UbQ/plastoQ_OxRdtase. [Graphical view]
KO	K00343.
Pfam	PF00361. Oxidored_q1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01770. NDH_I_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NUON1_AQUAE

Accession

Primary (citable) accession number: O67342

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 9, 2010
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program