O67336 (NUOH_AQUAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit H
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit H
NDH-1 subunit H

Gene names

Name:	nuoH
Ordered Locus Names:	aq_1315

Organism

Aquifex aeolicus (strain VF5)

Taxonomic identifier

224324 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone By similarity. HAMAP MF_01350
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01350
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential HAMAP MF_01350.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	NADH-quinone oxidoreductase subunit H HAMAP MF_01350		PRO_0000240053
Regions
	Transmembrane	12 – 32	21	Helical; Potential
	Transmembrane	84 – 104	21	Helical; Potential
	Transmembrane	118 – 138	21	Helical; Potential
	Transmembrane	156 – 176	21	Helical; Potential
	Transmembrane	193 – 213	21	Helical; Potential
	Transmembrane	247 – 267	21	Helical; Potential
	Transmembrane	274 – 294	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	O67336 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5229C05EFDBE9915	FASTA	336	36,923
	10 20 30 40 50 60 MEAIAYSLAF TFLKIVIVFS LALGIGAYLT WFERKLAGHI QNRLGPTVVG KFGILQPLAD 70 80 90 100 110 120 ALKLATKEVI IPRGADKPVY YAAVVMALVP SILLLTIIPF GPGFRVGNTY IEPIIADVNI 130 140 150 160 170 180 ALLLAFAFGS LSVYGTIFSG WASNSKYAFI GSLRKAAVVI AYEVVLGFSV LGVILLAGTL 190 200 210 220 230 240 STVGIVEAQI QKGVWFIFYQ PVAFILYLFC MLAESGRVPF DIQEAEAELV TGYNVEYGGM 250 260 270 280 290 300 KFGAFPLAEW YVNVIALSAI AVVLFFGGWD GPHIFGPLSP YFWFVFKTFA LVFFTLWLHW 310 320 330 TLPRFQAKDI TEIAWKILLP IAILNVIITA VVVYAF

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000657 Genomic DNA. Translation: AAC07299.1.
PIR	E70413.
RefSeq	NP_213900.1. NC_000918.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1192774.
GenomeReviews	Gene locus aq_1315 in contig AE000657_GR.
KEGG	aae:aq_1315.
NMPDR	fig|224324.1.peg.915.
PATRIC	20959814. VBIAquAeo85532_1024.
Phylogenomic databases
HOGENOM	HBG727670.
OMA	EADKFTY.
PhylomeDB	O67336.
ProtClustDB	CLSK2299659.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1315-MONOMER.
Family and domain databases
HAMAP	MF_01350. NDH1_NuoH. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1/FPO. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
KO	K00337.
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOH_AQUAE

Accession

Primary (citable) accession number: O67336

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program