Reviewed, UniProtKB/Swiss-Prot O67335 (NUCD2_AQUAE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit C/D 2
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit C/D 2
    NDH-1 subunit C/D 2

Gene names

Name:	nuoC2
Synonyms:	nuoCD, nuoD, nuoD2
Ordered Locus Names:	aq_1314

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01397
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits nuoB, CD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	In the N-terminal section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	NADH-quinone oxidoreductase subunit C/D 2 HAMAP MF_01397		PRO_0000358614
Regions
	Region	1 – 173	173	NADH dehydrogenase I subunit C By similarity
	Region	197 – 586	390	NADH dehydrogenase I subunit D By similarity

Sequences

	Sequence		Length	Mass (Da)
	O67335-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 2D25917FA02EEEAE	FASTA	586	67,893
	10 20 30 40 50 60 MKWVNKGTVE RVKQEFKDEV KYYETKHTKG FEVSHDFLKP LLKFLKERER FLHFVDMTCI 70 80 90 100 110 120 DFPEHPNRFQ GVYILYNPEE NERVIVKSWA KDGKLPTVED LWPGAKWAER EAYDMFGVVF 130 140 150 160 170 180 EGHENLRRMF MWEGYEHYPL RKDFPLQGIP EVELPSLTEV LHGRTDPPSH DFELVHTKLP 190 200 210 220 230 240 TLEDLERTEK ARLKKKAELV LNWGPLHPGT HGTIWFLFDL EGEKVVQSDV ILGQLHRGME 250 260 270 280 290 300 KLAENLHYFQ FIPYTDRMDY ISAICNELAY VETVERLLGV EVPEKARYIR TMFAELQRIN 310 320 330 340 350 360 SHLLWLGTGA LDLGALTVFL YAFREREKIM DIIEGNAGYR LTSCFLRIGG VHYDLAEGTL 370 380 390 400 410 420 DVVKHFIKDF PNRLKEYHTL LTRNRIWLRR TKDVGVITRE DVHNYGLSGP VARGSGVPYD 430 440 450 460 470 480 LRKLQPYAAY DEVEFDIPVG EVGDVYDRYL VRMEEMAQSV RIIEQCVQKL EKLPKDAPYL 490 500 510 520 530 540 NKEHPAVIPP KEDVFHDLES MVKSFRVVVH GEDAPPGEVY FAGENPRGEL GFFIYSKGGG 550 560 570 580 KPYRTRIRSG ALYNLSIFPK LIQGRTIADA IALLGSLDPV VGETDR

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07298.1.
PIR	D70413.
RefSeq	NP_213899.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1192773.
GenomeReviews	Gene locus aq_1314 in contig AE000657_GR.
KEGG	aae:aq_1314.
NMPDR	fig|224324.1.peg.914.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67335.
OMA	ENRTNVM.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1314-MON.
Family and domain databases
HAMAP	MF_01397. [Tree]
InterPro	IPR010219. NADH_DH_1_dsu. IPR001135. NADH_Q_OxRdtase_suD. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR020396. NADH_UbQ_OxRdtase_CS. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. False negative. PS00535. COMPLEX1_49K. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUCD2_AQUAE

Accession

Primary (citable) accession number: O67335

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)