Reviewed, UniProtKB/Swiss-Prot O67332 (KHSE_AQUAE)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	aq_1309

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Homoserine kinase HAMAP MF_00384		PRO_0000156546
Regions
	Nucleotide binding	84 – 94	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O67332-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 84A08FA3B881C3B5	FASTA	297	32,679
	10 20 30 40 50 60 MIKIEVPATT TNFGSGFDTF GLALSLTNTF SVDFSDKYEV QIEGYSSGIP KDQKNLFIKV 70 80 90 100 110 120 YKKTCQSIGK KPKPLKLIQE NRVPPARGLG SSATAIVGGI EAALALHKVE LPLKEKLKIA 130 140 150 160 170 180 FEFEKHPDNI IPAFVGGFTV CATSESGVIF KKLPFPEDIK IVFVIPDFEV STSEARRVLP 190 200 210 220 230 240 KKVELKEAVF NVQRSALFVS ALLTKDYKLL REAVRDKLHQ PYREKLVPGL SEAILVSYKE 250 260 270 280 290 GALATFLSGA GPTICSLTTE NEEKIGEAIR EVITKFSGYD AQVMVLKARN EGVKVYS

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07283.1.
PIR	A70413.
RefSeq	NP_213896.1.
3D structure databases
HSSP	HSSP built from PDB template 1H74 based on UniProtKB Q58504.
ModBase	Search...
Genome annotation databases
GeneID	1192770.
GenomeReviews	Gene locus aq_1309 in contig AE000657_GR.
KEGG	aae:aq_1309.
NMPDR	fig|224324.1.peg.911.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67332.
OMA	FDCLGAA.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1309-MON.
BRENDA	2.7.1.39. 189781.
Family and domain databases
HAMAP	MF_00384. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP_bd_CS. IPR000870. Homoserine_kin. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_AQUAE

Accession

Primary (citable) accession number: O67332

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)