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Protein

Nucleoside-triphosphatase THEP1

Gene

aq_1292

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. May hydrolyze nucleoside diphosphates with lower efficiency. Does not have kinase activity.UniRule annotation1 Publication

Catalytic activityi

NTP + H2O = NDP + phosphate.UniRule annotation1 Publication

Kineticsi

  1. KM=5.5 µM for ATP1 Publication
  2. KM=45 µM for GTP1 Publication

    Temperature dependencei

    Optimum temperature is 70-80 degrees Celsius.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 148ATPCurated
    Nucleotide bindingi102 – 1098ATPUniRule annotation

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-924-MONOMER.
    MetaCyc:MONOMER-17884.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoside-triphosphatase THEP1UniRule annotation (EC:3.6.1.15UniRule annotation)
    Short name:
    NTPase THEP1UniRule annotation
    Alternative name(s):
    Nucleoside triphosphate phosphohydrolaseUniRule annotation
    Gene namesi
    Ordered Locus Names:aq_1292
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    Proteomesi
    • UP000000798 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 178178Nucleoside-triphosphatase THEP1PRO_0000146709Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.2 Publications

    Protein-protein interaction databases

    STRINGi224324.aq_1292.

    Structurei

    Secondary structure

    1
    178
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65Combined sources
    Helixi13 – 2412Combined sources
    Helixi25 – 273Combined sources
    Beta strandi28 – 369Combined sources
    Beta strandi46 – 516Combined sources
    Beta strandi56 – 616Combined sources
    Beta strandi67 – 704Combined sources
    Beta strandi73 – 753Combined sources
    Helixi77 – 9721Combined sources
    Beta strandi102 – 1054Combined sources
    Helixi112 – 1143Combined sources
    Helixi116 – 12611Combined sources
    Beta strandi131 – 1366Combined sources
    Helixi144 – 1507Combined sources
    Beta strandi156 – 1594Combined sources
    Turni162 – 1676Combined sources
    Helixi168 – 1758Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YE8X-ray1.40A1-178[»]
    ProteinModelPortaliO67322.
    SMRiO67322. Positions 1-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO67322.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the THEP1 NTPase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105Q33. Bacteria.
    COG1618. LUCA.
    HOGENOMiHOG000006446.
    InParanoidiO67322.
    KOiK06928.
    OMAiVHKICSA.
    OrthoDBiEOG6N0HJM.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00796. NTPase_1.
    InterProiIPR003593. AAA+_ATPase.
    IPR004948. Nuc-triphosphatase_THEP1.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF03266. NTPase_1. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O67322-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIIITGEPG VGKTTLVKKI VERLGKRAIG FWTEEVRDPE TKKRTGFRII
    60 70 80 90 100
    TTEGKKKIFS SKFFTSKKLV GSYGVNVQYF EELAIPILER AYREAKKDRR
    110 120 130 140 150
    KVIIIDEIGK MELFSKKFRD LVRQIMHDPN VNVVATIPIR DVHPLVKEIR
    160 170
    RLPGAVLIEL TPENRDVILE DILSLLER
    Length:178
    Mass (Da):20,555
    Last modified:August 1, 1998 - v1
    Checksum:i13AE2F602F08150C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07294.1.
    PIRiG70411.
    RefSeqiNP_213886.1. NC_000918.1.
    WP_010880824.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07294; AAC07294; aq_1292.
    GeneIDi1192760.
    KEGGiaae:aq_1292.
    PATRICi20959786. VBIAquAeo85532_1010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07294.1.
    PIRiG70411.
    RefSeqiNP_213886.1. NC_000918.1.
    WP_010880824.1. NC_000918.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YE8X-ray1.40A1-178[»]
    ProteinModelPortaliO67322.
    SMRiO67322. Positions 1-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224324.aq_1292.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC07294; AAC07294; aq_1292.
    GeneIDi1192760.
    KEGGiaae:aq_1292.
    PATRICi20959786. VBIAquAeo85532_1010.

    Phylogenomic databases

    eggNOGiENOG4105Q33. Bacteria.
    COG1618. LUCA.
    HOGENOMiHOG000006446.
    InParanoidiO67322.
    KOiK06928.
    OMAiVHKICSA.
    OrthoDBiEOG6N0HJM.

    Enzyme and pathway databases

    BioCyciAAEO224324:GJBH-924-MONOMER.
    MetaCyc:MONOMER-17884.

    Miscellaneous databases

    EvolutionaryTraceiO67322.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00796. NTPase_1.
    InterProiIPR003593. AAA+_ATPase.
    IPR004948. Nuc-triphosphatase_THEP1.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF03266. NTPase_1. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VF5.
    2. "Thermophile-specific proteins: the gene product of aq_1292 from Aquifex aeolicus is an NTPase."
      Klinger C., Rossbach M., Howe R., Kaufmann M.
      BMC Biochem. 4:12-12(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
    3. "Crystal structure of THEP1 from the hyperthermophile Aquifex aeolicus: a variation of the RecA fold."
      Rossbach M., Daumke O., Klinger C., Wittinghofer A., Kaufmann M.
      BMC Struct. Biol. 5:7-7(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) IN COMPLEX WITH PHOSPHATE.

    Entry informationi

    Entry nameiNTPTH_AQUAE
    AccessioniPrimary (citable) accession number: O67322
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: August 1, 1998
    Last modified: December 9, 2015
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.