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Protein

Nucleoside-triphosphatase THEP1

Gene

aq_1292

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. May hydrolyze nucleoside diphosphates with lower efficiency. Does not have kinase activity.UniRule annotation1 Publication

Catalytic activityi

NTP + H2O = NDP + phosphate.UniRule annotation1 Publication

Kineticsi

  1. KM=5.5 µM for ATP1 Publication
  2. KM=45 µM for GTP1 Publication

    Temperature dependencei

    Optimum temperature is 70-80 degrees Celsius.1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi7 – 14ATPCurated8
    Nucleotide bindingi102 – 109ATPUniRule annotation8

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17884.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoside-triphosphatase THEP1UniRule annotation (EC:3.6.1.15UniRule annotation)
    Short name:
    NTPase THEP1UniRule annotation
    Alternative name(s):
    Nucleoside triphosphate phosphohydrolaseUniRule annotation
    Gene namesi
    Ordered Locus Names:aq_1292
    OrganismiAquifex aeolicus (strain VF5)
    Taxonomic identifieri224324 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    Proteomesi
    • UP000000798 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001467091 – 178Nucleoside-triphosphatase THEP1Add BLAST178

    Interactioni

    Subunit structurei

    Monomer.2 Publications

    Protein-protein interaction databases

    STRINGi224324.aq_1292.

    Structurei

    Secondary structure

    1178
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 6Combined sources5
    Helixi13 – 24Combined sources12
    Helixi25 – 27Combined sources3
    Beta strandi28 – 36Combined sources9
    Beta strandi46 – 51Combined sources6
    Beta strandi56 – 61Combined sources6
    Beta strandi67 – 70Combined sources4
    Beta strandi73 – 75Combined sources3
    Helixi77 – 97Combined sources21
    Beta strandi102 – 105Combined sources4
    Helixi112 – 114Combined sources3
    Helixi116 – 126Combined sources11
    Beta strandi131 – 136Combined sources6
    Helixi144 – 150Combined sources7
    Beta strandi156 – 159Combined sources4
    Turni162 – 167Combined sources6
    Helixi168 – 175Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1YE8X-ray1.40A1-178[»]
    ProteinModelPortaliO67322.
    SMRiO67322.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO67322.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the THEP1 NTPase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105Q33. Bacteria.
    COG1618. LUCA.
    HOGENOMiHOG000006446.
    InParanoidiO67322.
    KOiK06928.
    OMAiVHKICSA.
    OrthoDBiPOG091H1PN4.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00796. NTPase_1. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR004948. Nuc-triphosphatase_THEP1.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF03266. NTPase_1. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O67322-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIIITGEPG VGKTTLVKKI VERLGKRAIG FWTEEVRDPE TKKRTGFRII
    60 70 80 90 100
    TTEGKKKIFS SKFFTSKKLV GSYGVNVQYF EELAIPILER AYREAKKDRR
    110 120 130 140 150
    KVIIIDEIGK MELFSKKFRD LVRQIMHDPN VNVVATIPIR DVHPLVKEIR
    160 170
    RLPGAVLIEL TPENRDVILE DILSLLER
    Length:178
    Mass (Da):20,555
    Last modified:August 1, 1998 - v1
    Checksum:i13AE2F602F08150C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07294.1.
    PIRiG70411.
    RefSeqiNP_213886.1. NC_000918.1.
    WP_010880824.1. NC_000918.1.

    Genome annotation databases

    EnsemblBacteriaiAAC07294; AAC07294; aq_1292.
    GeneIDi1192760.
    KEGGiaae:aq_1292.
    PATRICi20959786. VBIAquAeo85532_1010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000657 Genomic DNA. Translation: AAC07294.1.
    PIRiG70411.
    RefSeqiNP_213886.1. NC_000918.1.
    WP_010880824.1. NC_000918.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1YE8X-ray1.40A1-178[»]
    ProteinModelPortaliO67322.
    SMRiO67322.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224324.aq_1292.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC07294; AAC07294; aq_1292.
    GeneIDi1192760.
    KEGGiaae:aq_1292.
    PATRICi20959786. VBIAquAeo85532_1010.

    Phylogenomic databases

    eggNOGiENOG4105Q33. Bacteria.
    COG1618. LUCA.
    HOGENOMiHOG000006446.
    InParanoidiO67322.
    KOiK06928.
    OMAiVHKICSA.
    OrthoDBiPOG091H1PN4.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17884.

    Miscellaneous databases

    EvolutionaryTraceiO67322.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00796. NTPase_1. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR004948. Nuc-triphosphatase_THEP1.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF03266. NTPase_1. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNTPTH_AQUAE
    AccessioniPrimary (citable) accession number: O67322
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: August 1, 1998
    Last modified: November 2, 2016
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.