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Protein

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Gene

murA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100UDP-N-acetylglucosamineUniRule annotation1
Active sitei124Proton donorUniRule annotation1 Publication1
Binding sitei311UDP-N-acetylglucosamineCombined sources1 Publication1
Binding sitei333UDP-N-acetylglucosamine; via carbonyl oxygenCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.7UniRule annotation)
Alternative name(s):
Enoylpyruvate transferaseUniRule annotation
UDP-N-acetylglucosamine enolpyruvyl transferaseUniRule annotation
Short name:
EPTUniRule annotation
Gene namesi
Name:murAUniRule annotation
Ordered Locus Names:aq_1281
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001788451 – 425UDP-N-acetylglucosamine 1-carboxyvinyltransferaseAdd BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1242-(S-cysteinyl)pyruvic acid O-phosphothioketalUniRule annotation1 Publication1

Proteomic databases

PRIDEiO67315.

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_1281.

Structurei

Secondary structure

1425
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 16Combined sources5
Beta strandi22 – 26Combined sources5
Helixi31 – 40Combined sources10
Helixi41 – 43Combined sources3
Beta strandi48 – 52Combined sources5
Helixi57 – 68Combined sources12
Beta strandi72 – 76Combined sources5
Beta strandi79 – 83Combined sources5
Helixi93 – 98Combined sources6
Helixi100 – 105Combined sources6
Helixi106 – 113Combined sources8
Beta strandi114 – 119Combined sources6
Helixi132 – 140Combined sources9
Beta strandi144 – 148Combined sources5
Beta strandi151 – 155Combined sources5
Beta strandi163 – 165Combined sources3
Helixi171 – 181Combined sources11
Beta strandi185 – 192Combined sources8
Helixi197 – 208Combined sources12
Beta strandi212 – 216Combined sources5
Beta strandi219 – 223Combined sources5
Beta strandi232 – 234Combined sources3
Helixi239 – 251Combined sources13
Beta strandi255 – 260Combined sources6
Helixi263 – 265Combined sources3
Helixi267 – 276Combined sources10
Beta strandi278 – 283Combined sources6
Beta strandi286 – 291Combined sources6
Beta strandi300 – 302Combined sources3
Helixi310 – 320Combined sources11
Beta strandi323 – 330Combined sources8
Helixi339 – 345Combined sources7
Turni346 – 348Combined sources3
Beta strandi351 – 354Combined sources4
Beta strandi357 – 361Combined sources5
Beta strandi370 – 372Combined sources3
Turni376 – 379Combined sources4
Helixi380 – 388Combined sources9
Beta strandi389 – 396Combined sources8
Helixi399 – 404Combined sources6
Helixi408 – 414Combined sources7
Beta strandi419 – 422Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YVWX-ray1.81A1-425[»]
3SWGX-ray1.81A1-425[»]
ProteinModelPortaliO67315.
SMRiO67315.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67315.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 32Phosphoenolpyruvate bindingCombined sources1 Publication2
Regioni129 – 133UDP-N-acetylglucosamine bindingCombined sources1 Publication5
Regioni170 – 172UDP-N-acetylglucosamine bindingCombined sources1 Publication3

Sequence similaritiesi

Belongs to the EPSP synthase family. MurA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDF. Bacteria.
COG0766. LUCA.
HOGENOMiHOG000075602.
InParanoidiO67315.
KOiK00790.
OMAiIRTAPHP.
OrthoDBiPOG091H01PG.

Family and domain databases

CDDicd01555. UdpNAET. 1 hit.
Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00111. MurA. 1 hit.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
IPR005750. UDP_GlcNAc_COvinyl_MurA.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01072. murA. 1 hit.

Sequencei

Sequence statusi: Complete.

O67315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNTTLYTYR DYFVIRGGKP LTGKVKISGA KNAALPIMFA TILTEEPCTI
60 70 80 90 100
TNVPDLLDVR NTLLLLRELG AELEFLNNTV FINPSINSFI TNQEIIRRMR
110 120 130 140 150
ASVLSLGPLL GRFGRAVVGL PGGCSIGARP IDQHLKFFKE AGADVEVREG
160 170 180 190 200
YVYVNLKEKR RVHFKFDLVT VTGTENALLY LASVPEESIL ENIALEPEVM
210 220 230 240 250
DLIEVLKKMG AHVKVEGRSA YVKGSENLKG FTHSVIPDRI EAGTFMVGAV
260 270 280 290 300
LTDGEILLEN ARINHLRAVV EKLKLIGGEV VEENGNLRVF RKESLRACDI
310 320 330 340 350
ETQVYPGFPT DMQAQFMALL SVAKGKSRIK ENIFEHRFHH AQELNRLGAN
360 370 380 390 400
ITVRGNTAYV EGVERLYGSE VYSTDLRASA SLVLAGLVAQ GETVVRDVYH
410 420
LDRGYEKLEE KLKKLGADIE RVSEL
Length:425
Mass (Da):47,260
Last modified:August 1, 1998 - v1
Checksum:iF6E82EDA6CBC0DD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07268.1.
PIRiH70410.
RefSeqiNP_213879.1. NC_000918.1.
WP_010880817.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07268; AAC07268; aq_1281.
GeneIDi1192753.
KEGGiaae:aq_1281.
PATRICi20959770. VBIAquAeo85532_1002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07268.1.
PIRiH70410.
RefSeqiNP_213879.1. NC_000918.1.
WP_010880817.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YVWX-ray1.81A1-425[»]
3SWGX-ray1.81A1-425[»]
ProteinModelPortaliO67315.
SMRiO67315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1281.

Proteomic databases

PRIDEiO67315.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07268; AAC07268; aq_1281.
GeneIDi1192753.
KEGGiaae:aq_1281.
PATRICi20959770. VBIAquAeo85532_1002.

Phylogenomic databases

eggNOGiENOG4105CDF. Bacteria.
COG0766. LUCA.
HOGENOMiHOG000075602.
InParanoidiO67315.
KOiK00790.
OMAiIRTAPHP.
OrthoDBiPOG091H01PG.

Enzyme and pathway databases

UniPathwayiUPA00219.

Miscellaneous databases

EvolutionaryTraceiO67315.

Family and domain databases

CDDicd01555. UdpNAET. 1 hit.
Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00111. MurA. 1 hit.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
IPR005750. UDP_GlcNAc_COvinyl_MurA.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01072. murA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURA_AQUAE
AccessioniPrimary (citable) accession number: O67315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.