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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.By similarity

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi129ZincBy similarity1
Metal bindingi132ZincBy similarity1
Metal bindingi147ZincBy similarity1
Metal bindingi150ZincBy similarity1
Binding sitei298ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Ordered Locus Names:aq_1257
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001392061 – 497Methionine--tRNA ligaseAdd BLAST497

Proteomic databases

PRIDEiO67298.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi224324.aq_1257.

Structurei

Secondary structure

1497
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 15Combined sources10
Helixi22 – 40Combined sources19
Beta strandi44 – 52Combined sources9
Helixi56 – 59Combined sources4
Helixi61 – 64Combined sources4
Beta strandi65 – 67Combined sources3
Helixi69 – 86Combined sources18
Beta strandi92 – 96Combined sources5
Helixi100 – 115Combined sources16
Beta strandi119 – 123Combined sources5
Beta strandi160 – 164Combined sources5
Helixi166 – 168Combined sources3
Helixi170 – 179Combined sources10
Helixi181 – 183Combined sources3
Helixi187 – 199Combined sources13
Beta strandi207 – 209Combined sources3
Turni210 – 212Combined sources3
Beta strandi215 – 219Combined sources5
Beta strandi222 – 227Combined sources6
Helixi230 – 234Combined sources5
Helixi236 – 239Combined sources4
Turni240 – 244Combined sources5
Helixi245 – 248Combined sources4
Beta strandi254 – 257Combined sources4
Helixi258 – 260Combined sources3
Helixi261 – 265Combined sources5
Helixi267 – 275Combined sources9
Beta strandi283 – 286Combined sources4
Beta strandi289 – 295Combined sources7
Turni298 – 301Combined sources4
Helixi306 – 313Combined sources8
Helixi315 – 324Combined sources10
Helixi336 – 345Combined sources10
Helixi346 – 351Combined sources6
Helixi352 – 365Combined sources14
Helixi378 – 393Combined sources16
Turni394 – 396Combined sources3
Helixi398 – 419Combined sources22
Helixi421 – 425Combined sources5
Helixi430 – 451Combined sources22
Turni452 – 454Combined sources3
Helixi456 – 465Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CSXX-ray2.70A/B1-497[»]
2CT8X-ray2.70A/B1-497[»]
ProteinModelPortaliO67298.
SMRiO67298.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67298.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi14 – 24"HIGH" regionAdd BLAST11
Motifi295 – 299"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0143. LUCA.
HOGENOMiHOG000200401.
InParanoidiO67298.
KOiK01874.
OMAiHTFYVWF.
OrthoDBiPOG091H0269.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.

Sequencei

Sequence statusi: Complete.

O67298-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLMKKFYVT TPIYYVNDVP HLGHAYTTIA ADTIARYYRL RDYDVFFLTG
60 70 80 90 100
TDEHGLKIQK KAEELGISPK ELVDRNAERF KKLWEFLKIE YTKFIRTTDP
110 120 130 140 150
YHVKFVQKVF EECYKRGDIY LGEYEGWYCV GCEEFKSEAE LAEDHTCPIH
160 170 180 190 200
QKKCEYIKEP SYFFRLSKYQ DKLLELYEKN PEFIQPDYRR NEIISFVKQG
210 220 230 240 250
LKDLSVTRPR SRVKWGIPVP FDPEHTIYVW FDALFNYISA LEDKVEIYWP
260 270 280 290 300
ADLHLVGKDI LRFHTVYWPA FLMSLGYELP KKVFAHGWWT VEGKKMSKTL
310 320 330 340 350
GNVVDPYEVV QEYGLDEVRY FLLREVPFGQ DGDFSKKAIL NRINGELANE
360 370 380 390 400
IGNLYSRVVN MAHKFLGGEV SGARDEEYAK IAQESIKNYE NYMEKVNFYK
410 420 430 440 450
AIEEILKFTS YLNKYVDEKQ PWALNKERKK EELQKVLYAL VDGLFVLTHL
460 470 480 490
LYPITPNKMK EALQMLGEKE FLKELKPYSK NTYKLGERKI LFPKREG
Length:497
Mass (Da):58,931
Last modified:August 1, 1998 - v1
Checksum:i846C68E72AFEB4F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07256.1.
PIRiG70408.
RefSeqiNP_213862.1. NC_000918.1.
WP_010880800.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07256; AAC07256; aq_1257.
GeneIDi1192736.
KEGGiaae:aq_1257.
PATRICi20959724. VBIAquAeo85532_0980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07256.1.
PIRiG70408.
RefSeqiNP_213862.1. NC_000918.1.
WP_010880800.1. NC_000918.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CSXX-ray2.70A/B1-497[»]
2CT8X-ray2.70A/B1-497[»]
ProteinModelPortaliO67298.
SMRiO67298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1257.

Proteomic databases

PRIDEiO67298.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07256; AAC07256; aq_1257.
GeneIDi1192736.
KEGGiaae:aq_1257.
PATRICi20959724. VBIAquAeo85532_0980.

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0143. LUCA.
HOGENOMiHOG000200401.
InParanoidiO67298.
KOiK01874.
OMAiHTFYVWF.
OrthoDBiPOG091H0269.

Miscellaneous databases

EvolutionaryTraceiO67298.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_AQUAE
AccessioniPrimary (citable) accession number: O67298
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.