ID   ILVC_AQUAE              Reviewed;         333 AA.
AC   O67289;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   16-JUN-2009, entry version 60.
DE   RecName: Full=Ketol-acid reductoisomerase;
DE            EC=1.1.1.86;
DE   AltName: Full=Acetohydroxy-acid isomeroreductase;
DE   AltName: Full=Alpha-keto-beta-hydroxylacil reductoisomerase;
GN   Name=ilvC; OrderedLocusNames=aq_1245;
OS   Aquifex aeolicus.
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=63363;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   MEDLINE=98196666; PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex
RT   aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- CATALYTIC ACTIVITY: (R)-2,3-dihydroxy-3-methylbutanoate + NADP(+)
CC       = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
CC   -!- CATALYTIC ACTIVITY: (2R,3R)-2,3-dihydroxy-3-methylpentanoate +
CC       NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-
CC       isoleucine from 2-oxobutanoate: step 2/4.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine
CC       from pyruvate: step 2/4.
CC   -!- SIMILARITY: Belongs to the ketol-acid reductoisomerase family.
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DR   EMBL; AE000657; AAC07240.1; -; Genomic_DNA.
DR   PIR; F70407; F70407.
DR   RefSeq; NP_213853.1; -.
DR   HSSP; Q9HVA2; 1NP3.
DR   GeneID; 1192727; -.
DR   GenomeReviews; AE000657_GR; aq_1245.
DR   KEGG; aae:aq_1245; -.
DR   NMPDR; fig|224324.1.peg.868; -.
DR   HOGENOM; O67289; -.
DR   OMA; O67289; AHGFNIR.
DR   BioCyc; AAEO224324:AQ_1245-MON; -.
DR   BRENDA; 1.1.1.86; 189781.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004455; F:ketol-acid reductoisomerase activity; IEA:HAMAP.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0009099; P:valine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00435; -; 1.
DR   InterPro; IPR013023; AcH_isomrdctse.
DR   InterPro; IPR000506; AcH_isomrdctse_C.
DR   InterPro; IPR013116; IlvN.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR21371; AcH_isomrdctse; 1.
DR   Pfam; PF01450; IlvC; 1.
DR   Pfam; PF07991; IlvN; 1.
DR   TIGRFAMs; TIGR00465; ilvC; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Complete proteome; NADP; Oxidoreductase.
FT   CHAIN         1    333       Ketol-acid reductoisomerase.
FT                                /FTId=PRO_0000151269.
FT   ACT_SITE    108    108       Potential.
SQ   SEQUENCE   333 AA;  37307 MW;  8E55F82619698B55 CRC64;
     MAKIYYDEDA SLDILKDKVI AILGYGSQGH AHALNLRDSG LNVIIGLHEG SRSREKAKAD
     GFEVYTPREA AKRADIIMFL IPDTVQPEVY KNEVEPELNS SKTLAFAHGF NIHFRQIVPP
     KDVDVFMVAP KGPGHLVRWM YTEGKGVPAL VAIHQDASGT CKDKALAYAK GIGATRAGVI
     ETTFKEETET DLFGEQMVLC GGVTALIKAG FETLVNAGYQ PEVAYFECLH ELKLIVDLIY
     EHGISGMRYS ISDTAKYGDV TRGERIYKVV KPVMEKTLEE IQKGEFAREW ILENKAGRPV
     YYALLERDRE HLVEKVGEEL RKMMPWLGKK ELK
//