Reviewed, UniProtKB/Swiss-Prot O67289 (ILVC_AQUAE)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	aq_1245

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151269
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O67289-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 8E55F82619698B55	FASTA	333	37,307
	10 20 30 40 50 60 MAKIYYDEDA SLDILKDKVI AILGYGSQGH AHALNLRDSG LNVIIGLHEG SRSREKAKAD 70 80 90 100 110 120 GFEVYTPREA AKRADIIMFL IPDTVQPEVY KNEVEPELNS SKTLAFAHGF NIHFRQIVPP 130 140 150 160 170 180 KDVDVFMVAP KGPGHLVRWM YTEGKGVPAL VAIHQDASGT CKDKALAYAK GIGATRAGVI 190 200 210 220 230 240 ETTFKEETET DLFGEQMVLC GGVTALIKAG FETLVNAGYQ PEVAYFECLH ELKLIVDLIY 250 260 270 280 290 300 EHGISGMRYS ISDTAKYGDV TRGERIYKVV KPVMEKTLEE IQKGEFAREW ILENKAGRPV 310 320 330 YYALLERDRE HLVEKVGEEL RKMMPWLGKK ELK

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000657 Genomic DNA. Translation: AAC07240.1.
PIR	F70407.
RefSeq	NP_213853.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Genome annotation databases
GeneID	1192727.
GenomeReviews	Gene locus aq_1245 in contig AE000657_GR.
KEGG	aae:aq_1245.
NMPDR	fig|224324.1.peg.868.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O67289.
OMA	O67289. AHGFNIR.
Enzyme and pathway databases
BioCyc	AAEO224324:AQ_1245-MON.
BRENDA	1.1.1.86. 189781.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_AQUAE

Accession

Primary (citable) accession number: O67289

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)