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Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi501Magnesium 1By similarity1
Metal bindingi508Magnesium 1By similarity1
Metal bindingi508Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:lysS
Ordered Locus Names:aq_1202
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001525951 – 597Lysine--tRNA ligaseAdd BLAST597

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi224324.aq_1202.

Structurei

3D structure databases

ProteinModelPortaliO67258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CRK. Bacteria.
COG1190. LUCA.
HOGENOMiHOG000236578.
InParanoidiO67258.
KOiK04567.
OMAiHHNTLDI.
OrthoDBiPOG091H0098.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 2 hits.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O67258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLLSSEIF FSAIQYNLSL MEEVRLKKLQ ELREKGINPY PYRFEVTDFI
60 70 80 90 100
GNIRKQYEEE PPENYKVRVK GVAKRVSRTE NGYMVRLADE KGIEILVFTK
110 120 130 140 150
EEGLKPKESY TFEGILKRVE GKLSLVEAVL TEEEGEEVYK IKEQFDYDPN
160 170 180 190 200
FRPVSLAGRL VSMRSMGKAI FGHIQDLTGK IQIYLKKDVI GEEKLKFFND
210 220 230 240 250
YIDVGDIVGV RGKLFRTNTG ELTVEVEEYQ LLAKSLHPLP EKWHGLKDVE
260 270 280 290 300
VRYRQRYLDL IANPEARRIF MLRTKLITEM RKFFEMHGFI EVETPILQPI
310 320 330 340 350
ASGANARPFV TYHNFLETEL YLRIAPELYL KRLIVGGFPR VYEIGKNFRN
360 370 380 390 400
ESVDRTHNPE FTMVEFYAAY WDYHDLIKFT EDMFVYLLEK TLGTLKVKYG
410 420 430 440 450
EWELDFSPPF KKVRYFDLLK EKTGKDKDFF LKDLEGLRKL AKELEIPDVE
460 470 480 490 500
RMTHAKLLDK VFEKVAEEDL IQPTFVIDFP KILSPLAKTH REDPDLVERF
510 520 530 540 550
ELIIARYEVA NAYTELNDPF DQKERFLEQL KEKQMGDEEA MDMDEDFIRA
560 570 580 590
LEYGMPPTAG EGIGIDRLVM ILANTDSIRE VILFPQLKPE KKKKEAP
Length:597
Mass (Da):70,024
Last modified:August 1, 1998 - v1
Checksum:i66D6DAB0D588819B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07218.1.
PIRiG70403.
RefSeqiNP_213822.1. NC_000918.1.
WP_010880760.1. NC_000918.1.

Genome annotation databases

EnsemblBacteriaiAAC07218; AAC07218; aq_1202.
GeneIDi1192604.
KEGGiaae:aq_1202.
PATRICi20959634. VBIAquAeo85532_0936.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA. Translation: AAC07218.1.
PIRiG70403.
RefSeqiNP_213822.1. NC_000918.1.
WP_010880760.1. NC_000918.1.

3D structure databases

ProteinModelPortaliO67258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_1202.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC07218; AAC07218; aq_1202.
GeneIDi1192604.
KEGGiaae:aq_1202.
PATRICi20959634. VBIAquAeo85532_0936.

Phylogenomic databases

eggNOGiENOG4105CRK. Bacteria.
COG1190. LUCA.
HOGENOMiHOG000236578.
InParanoidiO67258.
KOiK04567.
OMAiHHNTLDI.
OrthoDBiPOG091H0098.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 2 hits.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYK_AQUAE
AccessioniPrimary (citable) accession number: O67258
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.