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O67236 (O67236_AQUAE) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Name:purF EMBL AAC07202.1
Ordered Locus Names:aq_1175
OrganismAquifex aeolicus (strain VF5)
Taxonomic identifier224324 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site21For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2481Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding3941Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4441Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4471Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
O67236 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: DA1C57445F214023

FASTA46552,390
        10         20         30         40         50         60 
MCGVAGVFNS PYAAEYALLM IYSLQHRGQE SVGISSSDEK EIYTVKKPGR VLEAIKKEDL 

        70         80         90        100        110        120 
EKLKGSIAIA HVRYSTAGNA GGFNAQPLVR ETPLGKVAVV HNGNLVNFLT LRKELEDKGI 

       130        140        150        160        170        180 
KFNYNSDSEL FLALLEEGRT YIPEGVTLHP EDKDFLPYIF YALSRVKGAY SLIYLFRDKL 

       190        200        210        220        230        240 
VVARDPYGFR PLALGRKKNT ILVASESCAF DILEAEFWRE VKPGEVLVID KKGKRSYRIF 

       250        260        270        280        290        300 
SSPRKAMCIF EFVYFSRPDS YIFGDWVYNV RKEMGRQLAR EDNIEADVVI PVPDSGVVPA 

       310        320        330        340        350        360 
LGYAEEKGLP FEYGLIRNHY VGRSFIEPTP ELRNLRVLMK LSPNRAVLQG KRVIVIDDSL 

       370        380        390        400        410        420 
VRGTTSKRIV SMLRRAGAKE IHMRIASPPV VGPCYYGIDT PTKEELIANK MPIEDIAKFI 

       430        440        450        460 
GVDSLKYLSL EGLRKCVDKP EDFCDACFSL NYPVEVEDNV PSKIR

« Hide

References

[1]"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus."
Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.
Nature 392:353-358(1998) [PubMed: 9537320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VF5.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000657 Genomic DNA. Translation: AAC07202.1.
PIRA70401.
RefSeqNP_213800.1. NC_000918.1.

3D structure databases

HSSPHSSP built from PDB template 1AO0 based on UniProtKB P00497.
ProteinModelPortalO67236.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1192582.
GenomeReviewsGene locus aq_1175 in contig AE000657_GR.
KEGGaae:aq_1175.
NMPDRfig|224324.1.peg.815.
PATRIC20959586. VBIAquAeo85532_0913.

Phylogenomic databases

HOGENOMHBG392416.
OMAVWAPGEE.
PhylomeDBO67236.
ProtClustDBCLSK2299607.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR000583. GATase_2.
IPR017932. GATase_II.
IPR000836. PRibTrfase.
[Graphical view]
KOK00764.
PANTHERPTHR11907. Amd_phspho_trans. 1 hit.
PfamPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. PurF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameO67236_AQUAE
AccessionPrimary (citable) accession number: O67236
Entry history
Integrated into UniProtKB/TrEMBL: August 1, 1998
Last sequence update: August 1, 1998
Last modified: January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info